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Basal Verification of the Renal Rehabilitation to Establish Evidence

肾脏康复基础验证以建立证据

基本信息

批准号：
18300182
负责人：
KOHZUKI Masahiro
金额：
$ 10.82万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18300182/
关键词：
renal rehabilitation progressive renal disease long-term exercise branched amino acid angiotensin II receptor blocker renal protective effect improvement of exercise tolerability antihypertensive effect 腎臓リハビリテーション賢保護効果ラット腎保護作用

项目摘要

In experiment 1, Wistar-Kyoto (WKY) rats were subjected to 5/6-nephrectomy (5/6-NX), and the plasma concentration of branched amino acid (BCAA) of 5/6-NX rats was measured. The plasma BCAA concentration was significantly lower than that in the normal rats. On the other hand, the plasma BCAA concentration of 5/6-NX rats was normalized when we administered BCAA more than 0.375g/kg.In experiment 2, WKY rats were subjected to 5/6-NX, and were assigned to seven groups, according to the following treatments: 1) 5/6-NX without exercise (C); 2) 5/6-NX without exercise and receiving BCAA (0.375g/kg/day) (B); 3) 5/6-NX without exercise and receiving valsartan (10mg/kg/day) (V); 4) 5/6-NX with treadmill running at a speed of 20m/min for 60min/day, 5days/week (E); 5) V+E; 6) V+E+B; 7) sham operation without exercise (S). The rats were then treated for 12 weeks. The systolic blood pressure (SBP) and 24-hour urinary excretion of protein (UP) were measured at regular intervals. Twelve weeks after the initiation of treatments, the serum creatinine (Scr) and blood urea nitrogen (BUN) were measured. The soleus muscles were removed and the muscle fiber type was determined.The SBP, UP, Scr and BUN in the C group were significantly higher than those in the S group. On the other hand, the SBP and UP in the V and V+E groups and the SBP in the E group were significantly lower than those in the C group. Particularly, the SBP, UP, Scr and proportion of type-1 fiber in soleus muscle in the V+E+B group were significantly better than those in the C group.These results suggest that the simultaneous treatment of long-term exercise, valsartan and BCAA has effects to control blood pressure well, and to protect the remnant kidney, and to improve exercise tolerability in a rat model of chronic renal failure.

在实验1中，对Wistar-Kyoto（WKY）大鼠进行5/6-肾切除术（5/6-NX），并测量5/6-NX大鼠的支链氨基酸（BCAA）的血浆浓度。血浆BCAA浓度明显低于正常大鼠。实验二将WKY大鼠分为7组：1）5/6-NX不运动组（C组）; 2）5/6-NX不运动+BCAA组（D组）; 3）5/6-NX不运动+BCAA组（C组）（B）;（3）5/6-NX不运动并接受缬沙坦（10 mg/kg/d）（V）;（4）5/6-NX以20 m/min的速度跑台运动60 min/d，5天/周（E）;（5）V+E;（6）V+E+B）;（7）假手术不运动（S）。然后对大鼠进行12周的治疗。定期测量收缩压（SBP）和24小时尿蛋白排泄量（UP）。治疗12周后，测定血清肌酐（Scr）和血尿素氮（BUN）。C组收缩压（SBP）、尿蛋白（UP）、血肌酐（Scr）、尿素氮（BUN）均显著高于S组。另一方面，V组和V+E组的SBP和UP以及E组的SBP均显著低于C组。特别是V+E+B组的SBP、UP、Scr和比目鱼肌1型纤维比例明显优于C组，提示长期运动、缬沙坦和BCAA联合治疗对慢性肾功能衰竭大鼠血压有良好的控制作用，对残肾有保护作用，并能提高运动耐受性。