Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin on testicular function in premature rats.

2,3,7,8-四氯二苯并-对二恶英对早产大鼠睾丸功能的影响。

• 批准号: 14570732
• 负责人: OHYAMA Kenji
• 金额: $ 2.37万
• 依托单位: University of Yamanashi (2003)山梨医科大学 (2002)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14570732/
• 关键词: dioxin; testis; spermatogenesis; reproduction; spermiogenesis; estrogen receptor; Inhibin; androgen receptor; AhR; ARNT; Inhibin

We examined effects of persistent low and high dose of TCDD on testicular function in premature rats. 1) Effect of very low dose of TCDD administered to maternal rats on gonadal function of neonatal rats.We injected into maternal rats extremely 10 ng/kg of TCDD once a week from the time before the sex differentiation of fetus to the end of breast feeding, and observed the change in gene expression of testis and liver of the male offspring. 21days after birth neonatal rats ware subjected. Results : Testicular weights were increased at 3 weeks, but reduced at 6weeks in TCDD-treated rats. In morphological examination of testes there were no significant differences in cell numbers of germ cells, Sertoli cells and Leydig cells between controls and TCDD-treated rats at 3 and 6 weeks. The diameter of tubules were, however, significantly increased at 3 weeks in TCDD-treated rats. CYP1A1 mRNA in TCDD-treated rats was dramatically increased in the liver, not in the testis at 3 and 6 weeks. Compa … More red with control, the CYP1A1 mRNA expression were increased 10-fold at 3 weeks and 3-fold at 6 weeks in TCDD-treated liver. In contrast to CYP1A1, the levels of AhR and ARNT mRNA expression were not much influenced by the TCDD treatment. The expression of Inhibin alpha and beta-A mRNA in control testis decreased at 6 weeks, compared to those at 3 weeks. However TCDD did not alter the inhibin alpha, beta-A and beta-B mRNA expression at both 3 and 6 weeks. The level of ER alpha mRNA was statistically increased at 3 weeks in TCDD-treated testes compared with controls. There was no significant difference at 6 weeks between TCDD-treated testes and controls. Expression of ER beta was increased at 6 weeks in TCDD-treated testes, but not at 3 weeks. The TCDD treatment did not alter the level of testicular AR mRNA expression at 3 and 6 weeks. 2) Effects of high dose (1000 and 300 ng/kg/w from birth ot 8 weeks of age) of TCDD. Motility of sperm is decreased and abnormality of TCDD impairs testicular function, especially spermiogenesis. Less

研究了持续低、高剂量TCDD对早产大鼠睾丸功能的影响。1)母体大鼠极低剂量TCDD对新生大鼠性腺功能的影响。我们从胎儿性别分化前至母乳喂养结束，每周1次给母鼠注射极10 ng/kg的TCDD，观察雄性后代睾丸和肝脏基因表达的变化。新生大鼠出生后21天进行实验。结果：tcdd治疗大鼠睾丸重量在3周时增加，在6周时减少。在睾丸形态学检查中，3周和6周时，对照组和对照组大鼠的生殖细胞、支持细胞和间质细胞数量无显著差异。然而，tcdd治疗大鼠的小管直径在3周时明显增加。3周和6周，tcdd治疗大鼠肝脏中CYP1A1 mRNA显著升高，而睾丸中没有。与对照组相比，在tcdd治疗的肝脏中，CYP1A1 mRNA的表达在3周和6周时分别增加了10倍和3倍。与CYP1A1相比，TCDD治疗对AhR和ARNT mRNA表达水平影响不大。对照组睾丸中抑制素α和β - a mRNA的表达在6周时较3周时下降。然而，TCDD在3周和6周时均未改变抑制素α、β - a和β - b mRNA的表达。与对照组相比，tcdd治疗后3周睾丸ER α mRNA水平有统计学意义升高。6周时，tcdd治疗的睾丸与对照组无显著差异。在tcdd治疗的6周时，ER β的表达增加，但在3周时没有增加。TCDD治疗在3周和6周时没有改变睾丸AR mRNA的表达水平。2)出生至8周龄时高剂量（1000和300 ng/kg/w） TCDD的影响。精子活力下降，TCDD异常损害睾丸功能，尤其是精子发生。少

项目成果

太田正法: "極微量ダイオキシンの母体投与による新生仔への影響"日本内分泌学会雑誌. 78・1. 109-109 (2002)

Masanori Ota：“母亲服用超痕量二恶英对新生儿的影响”日本内分泌学会杂志78・1（2002）。

Sato K, Ohyama K, Nakagomi Y, et al.: "Effects of growth hormone on testicular dysfunction induced by cyclophosphamide in GH-deficient rats."Endocrine J. 49・6. 611-619 (2002)

Sato K、Ohyama K、Nakagomi Y 等：“生长激素对 GH 缺陷大鼠环磷酰胺诱导的睾丸功能障碍的影响”，Endocrine J. 49・6 (2002)。

大山建司: "第2次性決定機構の異常症、外因性ホルモン異常"日本臨床. 62・2. 379-384 (2004)

Kenji Oyama：“第二性别决定机制异常和外源性激素异常”日本临床研究 62・2（2004）。

Sato K, Ohyama K, Nakagomi Y, et al.: "Effects of growth hormone on testicular dysfunction induced by cyclophosphamide in GH-deficient rats."Endocrine J. 49(6). 611-619 (2002)

Sato K、Ohyama K、Nakagomi Y 等人：“生长激素对 GH 缺陷大鼠中环磷酰胺诱导的睾丸功能障碍的影响”。Endocrine J. 49(6)。

Yamano Y, Ohyama K, Sano T, et al.: "A novel spermatogenesis-related factor 1 gene expressed in maturing rat testis."Biochem Biophys Res Commun. 289(4). 888-893 (2001)

Yamano Y、Ohyama K、Sano T 等人：“一种在成熟大鼠睾丸中表达的新型精子发生相关因子 1 基因。”Biochem Biophys Res Commun。