Effect of sex hormone and IGF-I on skeletal qrowth and maturation--Activity of aromatase and 5-reductase in chondrocyte--

性激素和IGF-I对骨骼生长和成熟的影响--软骨细胞中芳香酶和5-还原酶的活性--

• 批准号: 01570519
• 负责人: OHYAMA Kenji
• 金额: $ 0.26万
• 依托单位: Yamanashi Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570519/
• 关键词: Aromatase; 5-alpha-reductase; IGF-I; Chondrocyte; Androgen; Estrogen; 軟骨細胞; 身長発育; 骨成長; 静止軟骨; 5α-リダクタ-ゼ活性

Using isolated rabbit chondrocytes, we investigated the effect of sex steroids produced by cartilarges on qrowth and maturation of bone. Both aromatase activity and 5-reductase activity existed in chondrocytes from ribs and articular cartilarges of 2-week-old Japanese white nibs. Chondrocytes from articular cartilarges had higher aromatase activity than those from rib cartilarges. In rib cartilarges, aromatase activity in growth plate was significantly higher than those in resting cartilarges. Preincubation of cultured chondrocytes with dexamethasone stimulated aromatase expression in all cell strains. The increment of dexamethasone-induced aromatase activity was significantly higher in chondrocytes from growth plate than in resting chondrocytes ( articular chondrocyte > rib growth chondrocyte > rib resting chondrocyte ), and was significantly higher in female chondrocyte than in male one. In contrast, 5-reductase activity was similar in each chondrocyte and in both sexes. Preincubation of chondrocytes with IGF-I did not induced activities of aromatase and 5-reductase.These results suggest that chondrocytes can produce estrogen and androgen, and estrogen production increases, and the maturation of cartilarge and bone may be advanced by paracrine action of those steroids, especially estrogen action.

利用分离的兔软骨细胞，我们研究了软骨产生的性类固醇对骨生长和成熟的影响。2周龄日本白尖骨的肋骨和关节软骨细胞均存在芳香化酶活性和5-还原酶活性。来自关节软骨的软骨细胞的芳香酶活性高于来自肋骨软骨的软骨细胞。肋软骨生长板中芳香酶活性显著高于静息软骨。用地塞米松预孵育培养的软骨细胞可刺激所有细胞株的芳香酶表达。生长板软骨细胞（关节软骨细胞>肋骨生长软骨细胞>肋骨休息软骨细胞）中地塞米松诱导的芳香酶活性的增加明显高于静息软骨细胞，且雌性软骨细胞明显高于雄性软骨细胞。相反，5-还原酶活性在每个软骨细胞和两性中是相似的。用igf - 1预孵育软骨细胞没有诱导芳香化酶和5-还原酶的活性。这些结果提示，软骨细胞可以产生雌激素和雄激素，并且雌激素的产生增加，这些类固醇的旁分泌作用，特别是雌激素的作用可能会促进软骨和骨的成熟。

项目成果

大山 建司: "小児科Mook(59):性の分化と成熟の異常" 金原出版(編集, (1990)

Kenji Oyama：“小儿 Mook(59)：性别分化和成熟异常”Kanehara Publishing（编辑），（1990 年）

大山 建司: "男児の血漿総SomatomedinーC濃度、血清Testosterone濃度の加齢変化に関する検討" 日本小児科学会雑誌. 93. 270-275 (1989)

Kenji Oyama：“男孩血浆总生长调节素 C 浓度和血清睾酮浓度随年龄变化的研究”日本儿科学会杂志 93. 270-275 (1989)。

矢守利次,藤本昌敏,大山建司 他: "培養軟骨細胞におけるアロマタ-ゼ活性及び5α-レダクタ-ゼ活性について" 日本内分泌学会雑誌. 65. 886- (1989)

Toshitsugu Yamori、Masatoshi Fujimoto、Kenji Oyama 等：“关于培养软骨细胞中的芳香酶活性和 5α-还原酶活性”日本内分泌学会杂志 65. 886- (1989)。

大山 建司: "成長の異常ー身長と骨年齢" 小児科臨床. 42. 789-797 (1989)

Kenji Oyama：“生长异常 - 身高和骨龄”儿科学 42. 789-797 (1989)。

大山 建司: "小児科Mook(59):性の分化と成熟の異常" 金原出版(編集), (1990)

Kenji Oyama：《Pediatric Mook(59)：性分化和成熟的异常》Kanehara Publishing（编辑），（1990）