Effects of Cyclophosphamide and Dioxin (TCDD) on spermatogenesis in rats

环磷酰胺和二恶英（TCDD）对大鼠精子发生的影响

• 批准号: 11670751
• 负责人: OHYAMA Kenji
• 金额: $ 2.43万
• 依托单位: Yamanashi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670751/
• 关键词: Growth Hormone; Spermatogenesis; Gyclophosphamide; Germ cell; Sperm; Dioxin; GH欠損ラット; ダイオキシン; Cyclophosphamide; 精子; 下垂体摘出ラット

Effects of Cyclophosphamide and Dioxin (TCDD) on spermatogenesis in rats1. Effects of GH on recovery from testicular damage induced by Cyclophosphamide (CP) in GH-deficient rats. CP was intravenously administered at dose of 50 mg/kg for 2 days, and rat GH was subcutaneously administered at dose of 0.3 mg/kg every day for 2 and 4 weeks. The number of spermatocytes was decreased when they exposed to CP at type A spermatogonia. The.motility index of spermatozoa was also decreased by CP. In CP/GH group, the number of those cells was recovered and the damaged motility of spermatozoa was improved. Under GH-deficient condition, exogeneous GH can improve the testicular function damaged by CP.2. Effect of very low dose of TCDD administered to maternal rats on ganadal function of neonatal rats. TCDD was subcutaneously administered at dose of 10 Rg/kg once a week to maternal rats (7, 14, 21 dpc, and 7, 14 days after birth). 21days after birth neonatal rats were subjected. All organs were normal by macroscopic examination, and testicular tissues were also microscopically normal. Expression of Cyp lAlspecifically induced by TCDD was markedly increased in neonatal rat liver. Inhibin B (marker of Sertoli cell function) expression in testis was normal.

环磷酰胺和二恶英对大鼠精子发生的影响1. GH对环磷酰胺（CP）诱导的GH缺乏大鼠睾丸损伤恢复的影响。CP以50 mg/kg剂量静脉给药2天，大鼠GH以0.3 mg/kg剂量每天皮下给药2周和4周。CP作用于A型精原细胞后，精母细胞数量减少。CP还可降低精子的活力指数。而CP/GH组则可使受损的精子活力恢复。在GH缺乏的情况下，外源性GH可以改善CP损伤的睾丸功能。极低剂量TCDD染毒对新生大鼠性腺功能的影响TCDD以10 μ g/kg的剂量每周一次皮下注射给母鼠（出生后7、14、21 dpc和7、14 d）。出生后21天的新生大鼠进行实验。肉眼检查所有器官均正常，睾丸组织在显微镜下也正常。TCDD特异性诱导的新生大鼠肝脏Cyp 1A 1表达明显增加。睾丸组织中抑制素B（支持细胞功能标志物）表达正常。

项目成果

Ogawa M, Ohyama K: "Prevalence of GH-1 gene deletion in patients with isolated growth hormone deficiency in Japan"Endocr J. 47 (2). 157-162 (2000)

Okawa M、Ohyama K：“日本孤立性生长激素缺乏症患者 GH-1 基因缺失的患病率”Endocr J. 47 (2)。

Yamano Y, Ohyama K: "A novel spermatogenesis-related factor-1 gene expressed in maturing testis"Biochem Biophys Res Commun. 289(4). 888-893 (2001)

Yamano Y、Ohyama K：“一种在成熟睾丸中表达的新型精子发生相关因子 1 基因”Biochem Biophys Res Commun。

Tanaka T, Ohyama K: "Growth-promoting effect of GH treatment at various doses in children with intrauterine growth retardation"Clin Pediatr Endocrinol. 10(supp15). 15-23 (2001)

Tanaka T、Ohyama K：“不同剂量的 GH 治疗对宫内生长迟缓儿童的生长促进作用”Clin Pediatr Endocrinol。

Ohyama K: "Restoration of seminiferous tubular function after discontinuation of long-term gonadotropin-releasing hormone agonist in premature male rats"Endocr J. 44(4). 459-465 (1997)

Ohyama K：“早产雄性大鼠长期停用促性腺激素释放激素激动剂后曲细精管功能的恢复”Endocr J. 44(4)。

Ohyama K: "Molecular basis and clinical manifestation of McCune-Albright syndrome"Clin Pediatr Endocrinol. 9(1). 1-5 (2000)

Ohyama K：“McCune-Albright 综合征的分子基础和临床表现”Clin Pediatr Endocrinol。