Specific gene expression and its functional analysis in the maturing rat testis.

精子发生是一个复杂的过程，许多基因系统地表达。我们利用差异显示技术筛选了4个成熟大鼠睾丸组织中的基因。Hrasls5基因产物的氨基酸序列推测其在精子发生过程中可能具有抑癌作用。RGD1303144基因主要在精母细胞中表达。结肠癌自身抗原基因Sdccag8也定位于7周龄大鼠睾丸精母细胞，并在精子细胞中检测到其蛋白。sdccag8可能在精母细胞向精子细胞的发育过程中起作用。原位杂交结果显示Ttc29主要在精母细胞中表达。Ttc29的表达被FSH处理上调。ttc29编码轴丝动力蛋白，它是精子鞭毛的一种成分。这些数据表明，轴丝动力蛋白的表达开始于精母细胞，并受到FSH的调节

Spermatogenesis is a complex process, and many genes are systematically expressed. We screened 4 genes in maturing rat testis using differential display method. Hrasls5 gene product might function as a tumor suppressor as well as in spermatogenesis, as deduced from its amino acid sequence. RGD1303144 gene was expressed dominantly in spermatocytes. Colon cancer autoantigen gene, Sdccag8, was also localized to the spermatocytes in 7-week-old rat testis, and its protein was detected in the spermatids. Sdccag8 may have a role in the development from spermatocyte to spermatid. In situ hybridization revealed that Ttc29 was expressed primarily in spermatocytes. Ttc29 expression was up-regulated by treatment with FSH. Ttc29 encodes axonemal dynein, which is a component of sperm flagella. These data indicate that axonemal dynein expression starts in spermatocytes and is regulated by FSH