Molecular biological analysis of myelodysplastic syndrome of the childhood onset using DNA microarray method

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We have very difficult cases to distinguish juvenile myelomonocytic leukemia (JMML) of the infancy with leukemoid reaction by virus infection, because we have not yet established useful diagnostic method to differentiate between these two diseases. In this study, we analyzed the gene expression pattern of CD34^+ cells of the bone marrow of patients with JMML using DNA microarray method. We extracted RNA from CD34^+ cells of the bone marrow of JMML patients before and after treatment. We applied amplified cRNA labeled with fluorescent to DNA microarray slide. We found that the expression level of FRAP(FKBP-rapamycin associated protein) and Syk(spleen tyrosine kinase) increased significantly after treatment and that the expression level of c-jun, TGF-beta receptor III(transforming growth factor-beta receptor III) and IL-2(interleukin 2) decreased significantly after treatment. All of these five genes are involved with intracellular signal transduction, transformation, differentiation, and propagation. We conclude that DNA microarray method is useful for examination of a change of gene expression in JMML patients before and after treatment.