Fundamental Research on the Development of a New Technology of Direct Hemoperfusion Aiming to Remove Immunosuppressive Cytokines from the Blood of Cancer-Bearing Hosts

我们对直接血液灌流新技术的开发进行了基础性研究，目的是去除肿瘤宿主血液中的免疫抑制细胞因子。对聚苯乙烯基复合纤维进行化学改性制备免疫抑制物质吸附（ISA）纤维。简而言之，在多聚甲醛存在的情况下，用n -甲基-α-氯乙酰胺在硫酸和硝基苯的混合物中处理复合纤维。通过填充0.4 g ISA纤维制备容量为2 ml的无菌圆柱形塑料柱。将KDH-8肝癌细胞皮下植入WKAH/Hm大鼠背部，制备荷瘤大鼠。用这些柱直接灌流荷瘤大鼠的血液，可显著降低肿瘤的生长。延长了他们的生存时间。这些柱能有效地去除荷瘤大鼠血液中的TGFb、VEGF和IL-6。

We have done a fundamental research on the development of a new technology of direct hemoperfusion aiming to remove immunosuppressive cytokines from the blood of cancer-bearing hosts. Immunosuppressive substance adsorption (ISA) fiber was prepared by chemical modification of a polystyrene-based composite fiber. In brief, the composite fiber is treated with N-methylol-α-chloroacetamide in a mixture of sulfuric acid and nitrobenzene in the presence of paraformaldehyde. Sterile cylindrical plastic columns with a capacity of 2 ml were prepared by filling 0.4 g of ISA fiber. Tumor-bearing rats were made by implanting KDH-8 cells (hepatocelllar carcinoma) subcutaneously into the back of WKAH/Hm rats. Direct hemoperfusion of tumor-bearing rats using these columns significantly reduced the tumor growth and. prolonged the survival of them. These columns efficiently removed TGFb, VEGF, and IL-6 from the blood of tumor-bearing rats.