Fundamental Research on the Development of a New Technology of Direct Hemoperfusion Aiming to Remove Immunosuppressive Cytokines from the Blood of Cancer-Bearing Hosts

我们已经做了一个基础研究的发展，直接血液灌流，旨在消除免疫抑制细胞因子从血液中的癌症宿主。通过对聚苯乙烯基复合纤维进行化学改性，制备了免疫抑制物质吸附（伊萨）纤维。在多聚甲醛存在下，在硫酸和硝基苯的混合物中，用N-羟甲基-α-氯乙酰胺处理复合纤维。通过填充0.4g伊萨纤维制备容量为2 ml的无菌圆柱形塑料柱。通过将KDH-8细胞（肝细胞癌）皮下植入WKAH/Hm大鼠的背部制成荷瘤大鼠。使用这些柱对荷瘤大鼠进行直接血液灌流显著降低了肿瘤的生长。延长了他们的生存时间。这些柱有效地从荷瘤大鼠的血液中去除TGFb、VEGF和IL-6。

We have done a fundamental research on the development of a new technology of direct hemoperfusion aiming to remove immunosuppressive cytokines from the blood of cancer-bearing hosts. Immunosuppressive substance adsorption (ISA) fiber was prepared by chemical modification of a polystyrene-based composite fiber. In brief, the composite fiber is treated with N-methylol-α-chloroacetamide in a mixture of sulfuric acid and nitrobenzene in the presence of paraformaldehyde. Sterile cylindrical plastic columns with a capacity of 2 ml were prepared by filling 0.4 g of ISA fiber. Tumor-bearing rats were made by implanting KDH-8 cells (hepatocelllar carcinoma) subcutaneously into the back of WKAH/Hm rats. Direct hemoperfusion of tumor-bearing rats using these columns significantly reduced the tumor growth and. prolonged the survival of them. These columns efficiently removed TGFb, VEGF, and IL-6 from the blood of tumor-bearing rats.