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Effects of treatment with direct hemoperfusion using a new cytokine abserbent to septic shock

使用新型细胞因子吸收剂直接血液灌流治疗感染性休克的效果

基本信息

批准号：
18591980
负责人：
TANIGUCHI Takumi
金额：
$ 2.59万
依托单位：
Kanazawa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591980/
关键词：
direct hemoperfusion cytokine endotoxin shock sepsis

项目摘要

This research examined effects of the extracorporeal treatment with a new absorbent, CTR, which removed inflammatory cytokines such as THE-alpha, IL-I beta and IL-6, on mortality rate and inflammatory responses to endotoxic shock in rats.<Methods>1) To evaluate the effects of the extracorporeal treatment with CTR immediately after endotoxin injection in rats.2) To evaluate the effects of the extracorporeal treatment with CTR 2 hr after endotoxin injection in rats.3) To evaluate the dose-related effects of the extracorporeal treatment with CI R after endotoxin injection in rats.<Results>1) The mortality rates at 8 hrs after endotoxin injection were 92%, 92%, and 14% for the endotoxemic, control column, and CTR treatment groups, respectively. Hypotension and elevated plasma cytokine concentrations and the infiltration of neutrophils of the lungs were less conspicuous in the CTR treatment group than in the other two groups.2) The mortality rates 8hrs after endotoxin injection were 83%, 83%, and 33% for the endotoxemic, control column, and CTR-posttreatment groups, respectively. The increases of interleukin-6 concentrations were less for the CTR-posttreatment group than the other two groups.3) The mortality rates 8hrs after endotoxin injection were 92%, 58%, 42% and 17% for the control column, quarter dose, half dose, and full dose CTR groups, respectively. The increases in plasma cytokine concentrations were smaller in the half dose and full dose CTR treatment groups than in the endotoxemic group.<Conclusion>Direct hemoperfusion apheresis with CTR column reduced mortality and had inhibitory effects on the inflammatory responses during endotoxemia. These findings suggest that extracorporeal blood purification with CTR column may be available to use for the patients with sepsis and/or endotoxemia.

本研究检查了使用新的吸收剂CTR进行体外治疗的效果，CTR去除了炎症细胞因子，如THE-alpha，IL-I β和IL-6，对大鼠内毒素休克的死亡率和炎症反应的影响。<Methods>1）评价在大鼠中注射内毒素后立即用CTR进行体外治疗的效果。2）评价在大鼠中注射内毒素后2小时用CTR进行体外治疗的效果。3）评价在大鼠中注射内毒素后用CI R进行体外治疗的剂量相关效果。<Results>1）对于内毒素血症、对照柱和CTR处理组，内毒素注射后8小时的死亡率分别为92%、92%和14%。CTR治疗组的低血压和血浆细胞因子浓度升高以及肺中性粒细胞浸润较其他两组不明显。2）内毒素血症组、对照组和CTR治疗后组注射内毒素后8小时的死亡率分别为83%、83%和33%。对照组、四分之一剂量组、半剂量组和全剂量组注射内毒素后8小时的死亡率分别为92%、58%、42%和17%。半剂量和全剂量CTR治疗组中血浆细胞因子浓度的增加小于内毒素血症组。<Conclusion>CTR柱直接血液灌流单采术降低了死亡率，并对内毒素血症期间的炎症反应具有抑制作用。这些结果表明，CTR柱体外血液净化可用于脓毒症和/或内毒素血症患者。