Regional blood flow in the chronically compressed rat spinal cord

长期受压大鼠脊髓的局部血流

• 批准号: 14571328
• 负责人: OGINO Masahiro
• 金额: $ 1.98万
• 依托单位: Dokkyo University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571328/
• 关键词: anterior column; autoradiography; blood flow; cervical spondylosis; choline acetyltransferase; immunohistochemistry; spinal cord

Cervical myelopathy is a common cause of neurological disability among the elderly; however, the exact mechanism for the insidious and progressive deterioration remains to be elucidated. To study the pathophysiology, we developed a simple experimental model reproducing the course. In rats, a thin sheet of expanding polymer was implanted microsurgically underneath the C5-C6 laminae. In the control group, the polymer sheet was removed immediately. Changes in motor functions were monitored for 25 weeks after the operation, with voluntary exercise activity measured by odometer attached to revolving cages, and forced running capability measured by duration of exercise on a rotating treadmill. Motor neurons were counted stereologically in continuous sections. In the compression group, the forced running capability deteriorated after a latent period of 17 weeks and progressively thereafter. In the control group, it stayed unchanged throughout 25 weeks. Course of the voluntary exercise was comparable between the groups. Motor neuron density in the compression group decreased significantly in 9 weeks (-20.3%) and 25 weeks (-35.5%), but not in 1 or 3 weeks. This practical model properly reproduces characteristic features of the clinical cervical myelopathy, with progressive motor disturbance after a latency and insidious neuronal loss preceding the symptoms. These changes may be caused by decreased spinal cord blood flow measured by autoradiography

脊髓型颈椎病是老年人神经功能障碍的常见原因；然而，这种隐蔽性和进行性恶化的确切机制仍有待阐明。为了研究病理生理学，我们建立了一个简单的实验模型来再现这一过程。在大鼠的C5-C6椎板下，用显微外科手术植入了一层膨胀的聚合物薄片。在对照组中，聚合物片立即被移除。术后25周监测运动功能的变化，通过安装在旋转笼上的里程表测量自愿运动活动，通过在旋转跑步机上运动的持续时间测量强制跑步能力。连续切片对运动神经元进行立体计数。压缩组在17周潜伏期后，强迫跑步能力逐渐恶化。在对照组中，它在25周内保持不变。自愿性锻炼的过程在两组之间具有可比性。压缩组运动神经元密度在第9周（-20.3%）和第25周（-35.5%）明显下降，但在第1周和第3周均无明显下降。这个实用的模型恰当地再现了临床颈椎病的特征，在潜伏期后出现进行性运动障碍，在症状出现之前出现隐匿的神经元丧失。这些变化可能是由放射自显像测量的脊髓血流量减少引起的

项目成果

Kim Haisa T, Kawamolo T, Kirino T, Wakai S.: "Delayed myelopathy induced by chronic compression in the rat spinal cord."Annals of Neurology. 55. 503-511 (2004)

Kim Haisa T、Kawamolo T、Kirino T、Wakai S.：“大鼠脊髓慢性受压引起的迟发性脊髓病。”《神经病学年鉴》。

Kim P, Haisa T, Kawamoto T, Kirino T, Wakai S: "Delayed myelopathy induced by chronic compression in the rat spinal cord."Annals of Neurology. 55. 503-511 (2004)

Kim P、Haisa T、Kawamoto T、Kirino T、Wakai S：“大鼠脊髓慢性受压引起的迟发性脊髓病。”《神经病学年鉴》。