Hormone regulation of c-erB-2 oncogene expression in endometrial cancer

众所周知，恶性转化是一个多步骤的过程，在这个过程中，对通常参与生长调节的基因的损害最终导致肿瘤的形成。本文试图在体外研究人子宫内膜癌细胞系中雌二醇与c-erbB-2癌基因的关系。我们检查了两个celline；石川细胞系（西田博士：图波大学）雌激素受体阳性（ER）和孕激素受体阳性（PR）， OMC-2（上田博士：大阪医学）既无ER也无PR。在10 ^<-8 bb0 M雌二醇浓度下，7天后石川细胞系子宫内膜癌细胞的增殖比对照组更受刺激。但在OMC-2中未观察到这种增殖现象。添加雌二醇浓度为10^<-8>M的石川细胞株c-erbB-2 mRNA表达，未添加雌二醇的石川细胞株培养14天后c-erbB-2 mRNA表达略有下降。两种情况下，OMC细胞株均未出现c-erbB-2 mRNA的表达。c-erbB-2 mRNA的表达在人子宫内膜癌中通过激素受体介导的细胞增殖过程中发挥重要作用。

It is known that malignant transformation is a multistep process in which damage to genes normally involved in growth regulation culminates in tumor formation.An attempt was made to elucidate the relationship between the estradiol and c-erbB-2 oncogene in the human endometrial cancer cell line in vitro. we examined two of celline ; Ishikasa cell line (Dr.Nishida : Tukuba Univ.) having estorogen reseptor positive (ER) and progesterone receptor positive (PR), and OMC-2 (Dr.Ueda : Osaka Med) having neiter ER nor PR.The proliferation of endometrial cancer cells of Ishikawa line was more stimulated after the 7 days than the control at concentraion 10 ^<-8>M estradiol. But such the proliferation phenomenon was not observed in OMC-2. Ishikawa cell line added estradiol at a concentration of 10^<-8>M was presented the c-erbB-2 mRNA expression, but Ishikawa cell line without estrdiol stimulation was slightly decreased after 14 days culture. OMC cell line was not presnted the c-erbB-2 mRNA expression in the both conditions. The c-erbB-2 mRNA expression can play an important role in the development of cell proliferation mediated throught the hormone receptor in the human endometrial cancer.