Sciatica due to disc herniation-A role of TNF-alpha-

椎间盘突出引起的坐骨神经痛-TNF-α的作用-

• 批准号: 14571391
• 负责人: YABUKI Shoji
• 金额: $ 1.86万
• 依托单位: Fukushima Medical university
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571391/
• 关键词: disc herniation; radicular pain; tumor necrosis factor-alpha; spinal dorsal horn neuron; sciatica; electrophysiology; TNF-alpha; 炎症性サイトカイン; WDRニューロン; 異所性放電; 後根神経節

The purpose of the current study was 1)to analyze the effects of exogenous TNF-alpha in rat dorsal horn neurons, and 2)to analyze the effects of neutralizing TNF-alpha on nucleus pulposus-induced abnormal nociresponses in rat dorsal horn neurons.1)Abnormal discharges were observed more frequently after application of TNF-alpha to nerve root compared with control (physiologic saline solution). Also, increase of the frequency of abnormal discharges in rat dorsal horn neurons after pinch stimulation was caused by application of TNF-alpha to nerve root. These results suggest that TNF-alpha might relate to radicular pain due to disc herniation.2)The frequency of the spike number and the time of afterdischarges after pinch stimulation in the nucleus pulposus application group was significantly increased compared with the control group. Anti-TNF-alpha antibodies prevented the increase of afterdischarges in the nucleus pulposus application group. It was clarified that application of anti-TNF-alpha antibodies to the disc herniation model significantly decreased the abnormal discharges in the dorsal horn neurons caused by nucleus pulposus. These results suggest that TNF-alpha may be a substance of causing radicular pain in disc herniation.

本研究的目的是1）分析外源性TNF-α对大鼠背角神经元的影响，2）分析中和TNF-α对髓核诱导的大鼠背角神经元异常伤害反应的影响。1）与对照组（生理盐水溶液）相比，将TNF-α应用于神经根后观察到更频繁的异常放电。此外，捏刺激后大鼠背角神经元异常放电频率的增加是由神经根应用TNF-α引起的。这些结果表明TNF-α可能与椎间盘突出引起的神经根性疼痛有关。2）与对照组相比，髓核应用组的棘波数和后放电时间显著增加。抗TNF-α抗体阻止髓核应用组中后放电的增加。明确了将抗TNF-α抗体应用于椎间盘突出模型显著减少了由髓核引起的背角神经元中的异常放电。提示TNF-α可能是引起椎间盘突出症神经根性疼痛的物质之一。

项目成果

矢吹省司, 恩田 啓, 菊地臣一: "脊髄後角ニューロンの異常発火からみた椎間板ヘルニアによる神経根性疼痛の病態"臨床脳波. 46・1. 33-39 (2004)

Shoji Yabuki、Kei Onda、Shinichi Kikuchi：“从脊髓背角神经元的异常放电看出的椎间盘突出引起的根性疼痛的病理学”临床脑电图 46・1（2004）。

Akira Onda, Shoji Yabuki, Shinichi Kikuchi: "Effects of neutralizing antibodies to tumor necrosis factor-alpha on nucleus pulposus-induced abnormal nociresponces in rat dorsal horn neurons"Spine. 28・10. 967-972 (2003)

Akira Onda、Shoji Yabuki、Shinichi Kikuchi：“肿瘤坏死因子-α 中和抗体对大鼠背角神经元髓核诱导的异常伤害反应的影响”Spine 967-972 (2003)。

Akira Onda, Michiko Hamba, Shoji Yabuki, Shinichi Kikuchi: "Exogenous tumor necrosis factor-α induces abnormal discharges in rat dorsal horn neurons"Spine. 27・15. 1618-1624 (2002)

Akira Onda、Michiko Hamba、Shoji Yabuki、Shinichi Kikuchi：“外源性肿瘤坏死因子-α 诱导大鼠背角神经元异常放电”Spine 27・1624（2002）。

Onda A: "Exogenous tumor necrosis factor-a induces abnormal discharges in rat dorsal horn neurons"Spine. 27. 1618-1624 (2002)

Onda A：“外源性肿瘤坏死因子-a 诱导大鼠背角神经元异常放电”脊柱。

Onda A: "Effects of neutralizing antibodies to tumor necrosis factor alpha on nucleus pulposus-induced abnormal nociresponses in rat dorsal horn neurons"Spine. 28. 967-972 (2003)

Onda A：“肿瘤坏死因子α中和抗体对大鼠背角神经元髓核诱导的异常伤害反应的影响”脊柱。