Contribution of alveolar lymphocytes to acute lung injury.

肺泡淋巴细胞对急性肺损伤的贡献。

• 批准号: 14571415
• 负责人: HASHIMOTO Hiroshi
• 金额: $ 2.18万
• 依托单位: Hirosaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571415/
• 关键词: alveolar macrophage; alveolar lymphocyte; cytokine; adhesion molecules; acute lung injury; flow cytometry; operative magnitude; inflammation

1.Effects of alveolar lymphocytes on mild hypercapneaTo evaluate the mild hypercapnea on alveolar cell functions, we studied anesthetic patients maintained mild hypercapnea or normocapnea.To harvest alveolar cells were performed immediately after induction, 2,4,and 6 hours after induction of anesthesia, and at the end of the surgery. Phagocytic and microbicidal activities were evaluated. Also, harvested cells were dissolved in guanidinium buffer. Total RNA was isolated and cDNA was synthesized with a reverse transcriptase. By specific primers of interleukin-1beta and 8,interferon-gamma, tumor necrosis factor-alpha, semi-quantitative analysis was performed by polymerase chain reaction.We analyzed neutrophil and macrophage derived adhesion molecules including CD10a,b,c,CD18,49d,and 54,and lymphocyte derived adhesion molecules(CD 4 and 8) by a flowcytometry.Inter-group differences were not detected in any parameters of phagocytic and microbicidal functions. However, Expression of pro-infl … More ammatory cytokines, activated lymphocytes and neutrophil and macrophage derived adhesion molecules were much greater in hypercapneic patients than in normocapneic patients.Our results suggest that mild hypercapnea may protect pulmonary inflammatory reactions during anesthesia and surgery.2.Operative magnitude seriously modulates alveolar cell functions.We evaluated the effects of operative magnitude on alveolar macrophages functions.We studied patients undergoing major, moderate and minor surgery. Study protocol was described previously. Expression of pro-inflammatory cytokines, activated lymphocytes, and adhesion molecules significantly increase as the operative magnitude increased. The decrease in phagocytic and microbicidal activities was much greater in patients undergoing major surgery.These results suggest that increase in operative magnitude seriously aggravate pulmonary functions.3.Perioperiative hypothermia decreases all alveolar cell functions.We evaluated the effects of operative magnitude on alveolar macrophages functions.We studied perioperative hypothermic and normothermic patients. All alveolar cell functions decreased by hypothermia.These results may imply increase in rate of postoperative pulmonary infections. Less

1.肺泡淋巴细胞对轻度高碳酸血症的影响为了评价轻度高碳酸血症对肺泡细胞功能的影响，我们研究了维持轻度高碳酸血症或正常碳酸血症的麻醉患者，在麻醉诱导后即刻，麻醉诱导后2，4，6小时和手术结束时收集肺泡细胞。评价了吞噬和杀微生物活性。此外，将收获的细胞溶解在胍缓冲液中。分离总RNA，用逆转录酶合成cDNA。以白细胞介素-1 β和白细胞介素-8、干扰素-γ、肿瘤坏死因子-α为特异性引物，采用聚合酶链反应进行半定量分析，检测中性粒细胞和巨噬细胞来源的粘附分子CD 10 a、B、c、CD 18、49 d和54，和淋巴细胞衍生的粘附分子（CD 4和CD 8）。吞噬和杀微生物功能的任何参数均未检测到组间差异。然而，前炎症的表达 ...更多信息 结果表明，轻度高碳酸血症可保护麻醉和手术期间的肺部炎症反应。2.手术幅度严重调节肺泡细胞功能。我们评估了手术幅度对肺泡巨噬细胞功能的影响。我们研究了接受大手术的患者，中、小手术。先前描述了研究方案。促炎细胞因子、活化淋巴细胞和粘附分子的表达随着手术量的增加而显著增加。围手术期低温降低了肺泡巨噬细胞的各项功能。我们研究了围手术期低温和常温患者的肺泡巨噬细胞功能。低温可使肺泡细胞功能下降，提示术后肺部感染的发生率增加。少

项目成果

Kotani Naoki et al.: "Systemic heparinization during postoperative pulmonary embolism induces fatal complications."European Journal of Anaesthesiology. 19. 382-384 (2001)

Kotani Naoki 等人：“术后肺栓塞期间的全身肝素化会引起致命的并发症。”欧洲麻醉学杂志。

Kotani N, Kudo R, Sakurai Y, Sawamura D, Sessler DI, Okada H, Nakayama H, Yamagata T, Yasujima M, Hashimoto H, Matsuki A.: "Cerebrospinal fluid interleukin 8 concentrations and the subsequent development of postherpetic neuralgia."American Journal of Medi

Kotani N、Kudo R、Sakurai Y、Sawamura D、Sessler DI、Okada H、Nakayama H、Yamagata T、Yasujima M、Hashimoto H、Matsuki A.：“脑脊液白细胞介素 8 浓度与带状疱疹后神经痛的后续发展。”美国

Kotani N, Kushikata T, Suzuki A, Hashimoto H, Muraoka M, Hashimoto H, Matsuki A.: "Insertion of intradermal needles into painful points provides analgesia for intractable abdominal scar pain."Regional Anesthesia and Pain Medicine. 26(6). 532-538 (2001)

Kotani N、Kushikata T、Suzuki A、Hashimoto H、Muraoka M、Hashimoto H、Matsuki A.：“将皮内针插入疼痛点可为顽固性腹部疤痕疼痛提供镇痛。”区域麻醉和疼痛医学。

Kotant N, Hashimoto H, Kushikata T, Muraoka M, Tonosaki M, Matsuki A.: "Rebound perioperative hyperkalemia in six patients after cessation of ritodrine for premature labor."Anesthesia and Analgesia. 93(3). 709-711 (2001)

Kotant N、Hashimoto H、Kushikata T、Muraoka M、Tonosaki M、Matsuki A.：“因早产停止使用利托君后，六名患者出现围术期高钾血症反弹。”麻醉和镇痛。

Kotani N, Hashimoto H, Sato Y, Sessler DI, Yoshioka H, Kitayama M, Yasuda T, Matsuki A: "Preoperative intradermal acupuncture reduces postoperative pain, nausea and vomiting, analgesic requirement, and sympatho-adrenal responses."Anesthesiology. 95(2). 34

Kotani N、Hashimoto H、Sato Y、Sessler DI、Yoshioka H、Kitayama M、Yasuda T、Matsuki A：“术前皮内针灸可减少术后疼痛、恶心和呕吐、镇痛需求和交感肾上腺反应。”麻醉学。