A long duration follow up of childern born to patients With systematic lupus erythematosus (SLE)

小是众所周知的，因为它对前SLE的免疫学异常现象进行了规范。在我们的研究中，反核抗体（ANA）的阳性率明显高于SLE患者的儿童（SLE儿童：27.8%; 241人的67人），与控制因素相比（7.4%; 54人的4人）。最近，有报道称，SLE患者对发展疾病的风险估计比一般人口的风险高出20次。根据SLE儿童可能会在未来发展SLE，一项后续研究将开始分析SLE儿童中免疫和相关变化的课程，我们调查了SLE儿童是1991年至1999年期间的非患者诊所的SLE儿童。241个SLE儿童可以探测到ANA。在跟进期间， ANA在9个案件中从4岁到8岁改变了积极的态度。出于这个原因，我们分析了CD 4/CD 8、CD 3/CD 19（T细胞/B细胞）比率和CD 25、HLA-DR、CD 80/CD 86、外围血液的模糊表达 ... More 1 - 10流式细胞中SLE儿童和控制的非核细胞（PBMC）。1）SLE儿童过渡性退化在3-4岁的CD 4/CD 8比率。2）SLE儿童中的CD 4/CD 8 ANA阳性比率（14例）明显低于ANA阴性比率（58例）。（3）HLA-DR表达在CD 3阳性细胞之间存在显著差异，每个SLE儿童年龄段之间存在显著差异。4） Fas， CD80 and CD86 expression on CD3 positive cells of SLE children are significantly lower than that of controlls。在SLE模型鼠标中。Fas expression on CD5 positive B cells was biphsic with low （Fas D1 low） and high（Fas D1 high）。Fas-D1 low-D1 population是相对抵抗力到Fas mediated apoptosis and presumably autoantibody-secreting cells。对11个SLE儿童（ANA阳性： 2，阴性： 9）中的FASI-D1-Low-D1人口，CD 5阳性B细胞在PBMC上被刺激了4天的抗CD 40 mAb，现在已经进行了细胞测试。只有1个或11个显示出双相模式，谁能检测到ANA？免疫性细胞上的表型变化在ANA生产前和生产后都是罕见的。“当你走的时候，没有人被证明是清白的。以下是一个建议，即免疫学变化的许多步骤。“进一步的研究仍然是必要的。Less（低）

Little is known regarding the immunological abnormalities in pre-ictal SLE. In our study, the positive rate of antinuclear antibodies (ANA) is significantly higher in children born to patients with SLE (SLE children: 27.8%; 67 of 241) compared with controls (7.4%; 4 of 54). Recently, it is reported that the risk for siblings of SLE patients to develop disease is estimated to be 20 times higher than that of general population. Based on the speculation that SLE children may be prone to develop SLE in future, a follow-up study was started to analyze the course of the immmunoregulatory change in SLE children.We investigated SLE children who were invited the outpatients clinic of the Juntendo hospital in the period from 1991 to 1999. 67 of 241 SLE children had detectable ANA. During the follow up, ANA changed to the positive in 9 cases aged from 4 to 8. In this reason, we analyzed CD4/CD8, CD3/CD19 (T cell/B cell) ratios and CD25, HLA-DR, CD80/CD86, Fas expression on the peripheral blood mo … More nonuclear cells (PBMC) in SLE children and controls aged from 1 to 10 by flow cytometry. 1) The CD4/CD8 ratios of SLE children transiently decreased in age 3-4. 2) Among SLE children, The CD4/CD8 ratios of ANA positive (14 cases) are significantly lower than that of ANA negative (58 cases). 3) There are significant differences of HLA-DR expression on CD3 positive cells between each age of SLE children. 4) Fas, CD80 and CD86 expression on CD3 positive cells of SLE children are significantly lower than that of controls. In SLE model mouse. Fas expression on CD5 positive B cells was biphsic with low (FasィイD1lowィエD1) and high(FasィイD1highィエD1). FasィイD1lowィエD1 population was relatively resistant to Fas mediated apoptosis and presumably autoantibody-secreting cells. To examined FasィイD1lowィエD1 population in 11 SLE children (ANA positive: 2, negative: 9), CD5 positive B cells on PBMC were stimulated with anti-CD40 mAb for 4 days and examined by now cytometry. Only 1 out or 11 had shown biphasic pattern, who had detectable ANA. The phenotypic change on immunoregulatory cells was occurred before and after production of ANA. During follow up, nobody was dignosed define SLE. This suggests that there are many steps of immunological changes to develop SLE. Further study remains to be necessary. Less