3 dimensional fat regeneration using Adipose derived stem cells
使用脂肪干细胞进行 3 维脂肪再生
基本信息
- 批准号:14571723
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2002
- 资助国家:日本
- 起止时间:2002 至 2004
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The species of adipose derived stem cells was changed from human to GFP transgenic mice in this project because human specimen was relatively difficult to obtain. Accordingly, we firstly identified that similar population of human adipose derived stem cells(ASC) can be isolated from GFP transgenic mice.After the proliferation of ASC in control medium, they were induced into adipogenic differentiation in the induction medium for 1 week, followed by implanted subcutaneously into athymic mice with vehicles including non-woven polyglycolic acid, type I collagen sponge, hyaluronic acid and fibrin glue for in vivo adipogenesis. After 4 and 8 weeks of implantation, these specimen were evaluated macroscopically, histologically and immunohistochemically.After 4 weeks of implantation, ASC with fibrin glue revealed macroscopically half transparent with small angiogenesis. Furthermore, after 8 weeks of implantation, this specimen revealed yellish in color with rounder shape. In addition, these specimens strongly emitted green fluorescence through fluorescent microscope, which suggested that transplanted ASC contributed to in vivo adipogenesis. These findings were also confirmed by immunnohistochemical analysis using anti GFP monoclonal antibodies. Both H&E and Oil Red 0 staining revealed that 3 dimensional adipose tissue which contains small size of lipid droplets in each cell were engineered.In addition to fibrin glue, ASC with type I collagen sponge also contributed to in vivo adipogenesis, which may be another suitable scaffold for adipose tissue engineering. On the other hand, we didn't confirm any adipogenesis using other scaffolds. The gene expression of PPARγ using RT-PCR is currently being investigated.Finally, real-time PCR analysis revealed that ASC derived from female GFP mice has a 1.54 fold greater potential of gene expression of PPARγ compared with that from male mice.
由于人体脂肪干细胞的获取相对困难,本项目将脂肪干细胞的种属从人改为GFP转基因小鼠。因此,我们首次从GFP转基因小鼠中分离到了类似的人脂肪干细胞(ASC)群体,ASC在对照培养基中增殖后,在诱导培养基中诱导其向成脂分化1周,随后将载体包括无纺聚乙醇酸、I型胶原海绵、透明质酸和纤维蛋白胶用于体内脂肪形成。植入4周和8周后,肉眼观察、组织学和免疫组化检查,植入4周后,纤维蛋白胶处理的ASC肉眼可见半透明,有少量血管生成。此外,在植入8周后,该样本显示为黄色,形状更圆。荧光显微镜下观察到这些标本发出强烈的绿色荧光,提示移植的ASC有助于体内脂肪形成。这些发现也证实了使用抗GFP单克隆抗体的免疫组织化学分析。油红0染色和H&E染色结果显示,构建的脂肪组织具有三维结构,每个细胞内含有较小的脂滴,除纤维蛋白胶外,ASC和I型胶原海绵也有助于体内脂肪的形成,可能是脂肪组织工程的另一种合适的支架材料。另一方面,我们没有证实任何脂肪形成使用其他支架。实时荧光定量PCR结果显示,雌性GFP小鼠的ASC中PPAR γ基因的表达量是雄性GFP小鼠的1.54倍。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Osteogenic and chondrogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice
- DOI:10.1016/j.bbrc.2003.12.017
- 发表时间:2004-01-23
- 期刊:
- 影响因子:3.1
- 作者:Ogawa, R;Mizuno, H;Hyakusoku, H
- 通讯作者:Hyakusoku, H
Adipogenic differentiation by adipose-derived stem cells harvested from GFP transgenic mice - including relationship of sex differences
- DOI:10.1016/j.bbrc.2004.05.021
- 发表时间:2004-06-25
- 期刊:
- 影响因子:3.1
- 作者:Ogawa, R;Mizuno, H;Shimada, T
- 通讯作者:Shimada, T
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MIZUNO Hiroshi其他文献
MIZUNO Hiroshi的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('MIZUNO Hiroshi', 18)}}的其他基金
Establishment of iPS cells derived from keloid patients for the purpose of drug discovery
建立源自瘢痕疙瘩患者的 iPS 细胞用于药物发现
- 批准号:
26670778 - 财政年份:2014
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Mechanism of angiogenesis induced by adipose-derived stem cells
脂肪干细胞诱导血管生成的机制
- 批准号:
19592080 - 财政年份:2007
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Periodontal tissue regeneration by adipose derived stem cells
脂肪干细胞的牙周组织再生
- 批准号:
17591878 - 财政年份:2005
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
相似国自然基金
FAT1缺失上调CUL4A介导肺鳞癌化疗抵抗的机制研究
- 批准号:QN25H160122
- 批准年份:2025
- 资助金额:0.0 万元
- 项目类别:省市级项目
类泛素蛋白FAT10通过促进CSTF2诱导PD-L1 3'UTR缩短介导肿瘤免疫逃逸的机制研究
- 批准号:
- 批准年份:2024
- 资助金额:0 万元
- 项目类别:地区科学基金项目
FAT4调控骨髓微环境中CD100介导多发性骨髓瘤卡非佐米耐药的
机制研究
- 批准号:
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
FAT1 基因突变通过增强 DNA 修复蛋白的活性导致头颈鳞状
细胞癌耐药的机制研究
- 批准号:2024JJ9556
- 批准年份:2024
- 资助金额:0.0 万元
- 项目类别:省市级项目
UBE2C介导FAT1泛素化类型串扰诱导CD8+T细胞耗竭促进膀胱癌淋巴转移的机制研究
- 批准号:
- 批准年份:2024
- 资助金额:30.0 万元
- 项目类别:省市级项目
IL-32通过FAT4介导调控Hippo/YAP信号通路在椎间盘退变中的作用及机制研究
- 批准号:82302737
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
类泛素蛋白FAT10通过AMPK信号通路诱导自噬在乳腺癌他莫昔芬耐药中的作用及机制研究
- 批准号:82360602
- 批准年份:2023
- 资助金额:31.00 万元
- 项目类别:地区科学基金项目
CaMKII介导FAT/CD36调控衰老心肌脂毒性损伤的机制研究
- 批准号:82300334
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
肿瘤抑制因子FAT4负调控β-catenin激活抗宫颈癌免疫反应的机制研究
- 批准号:82303141
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
复黄生肌愈创油介导FAT10类泛素化修饰促进糖尿病溃疡上皮化愈合的机制研究
- 批准号:
- 批准年份:2022
- 资助金额:30 万元
- 项目类别:青年科学基金项目
相似海外基金
How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
- 批准号:
BB/Z514391/1 - 财政年份:2024
- 资助金额:
$ 2.24万 - 项目类别:
Training Grant
SBIR Phase I: Industrial-Scale Technology for Drug Development in Mature Human Fat Cells
SBIR 第一阶段:成熟人类脂肪细胞药物开发的工业规模技术
- 批准号:
2322443 - 财政年份:2024
- 资助金额:
$ 2.24万 - 项目类别:
Standard Grant
女性アスリートのコンディショニングを目指したFATのバイオマーカー探索と病態解析
女运动员体能FAT生物标志物搜索及病理分析
- 批准号:
23K27968 - 财政年份:2024
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Regulation of hepatic fat synthesis, turnover and transport by arylacetamide deacetylase (AADAC)
芳基乙酰胺脱乙酰酶 (AADAC) 对肝脂肪合成、周转和运输的调节
- 批准号:
478266 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Operating Grants
A novel neural pathway of preferential food selection for high carbohydrate and fat diets.
高碳水化合物和脂肪饮食的优先食物选择的新神经通路。
- 批准号:
23H02965 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Understanding plant based fat crystalization for the design of vegan and low carbon footprint chocolate
了解植物脂肪结晶在纯素和低碳足迹巧克力设计中的应用
- 批准号:
2894776 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Studentship
Understanding Plant Based Fat Crystallization for the Design of Vegan and Low Carbon Footprint Chocolate
了解植物脂肪结晶在纯素和低碳足迹巧克力设计中的应用
- 批准号:
BB/Y512692/1 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Training Grant
Milk fat globule-EGF factor 8 and hepatocyte apoptosis-induced liver wound healing response
乳脂肪球-EGF因子8与肝细胞凋亡诱导的肝脏创面愈合反应
- 批准号:
10585802 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Effects of maternal deficiency or excess of fat-soluble vitamin during pregnancy and lactation on the adipose tissue development of offspring
孕期和哺乳期母体脂溶性维生素缺乏或过量对子代脂肪组织发育的影响
- 批准号:
22KJ0135 - 财政年份:2023
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for JSPS Fellows