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Cell biology of taste buds and analysis of taste transduction mechanisms

味蕾细胞生物学及味觉传导机制分析

基本信息

批准号：
14571740
负责人：
TOYOSHIMA Kuniaki
金额：
$ 1.86万
依托单位：
Kyushu Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571740/
关键词：
ATP ATP receptors P2X3 P2Y1 RT-PCR immunohistochemistry in situ hybridization IP3R3 味蕾味覚うま味 P2Xs P2Ys mGluR4 ガストデューシン

项目摘要

Extracellular nucleotides such as ATP are the signaling molecules which bind to membrane receptors (P2X ligand-gated ion channels and G-protein coupled P2Y families). In the gustatory system, P2X receptors (P2X_2 and P2X_3) are expressed exclusively in nerve fibers innervating taste buds. Also, P2Y receptors are appeared to play some roles in taste signal transductions in taste bud cells on the basis of the physiological studies. However, the expression patterns of P2Y receptor subtypes have not been revealed. In this study, the expression patterns of P2Y_1 receptor subtypes were examined by using RT PCR, immunohistochemistry and in situ hybridization in rat gustatory papillae. These analyses showed that P2Y_1 receptor was expressed in taste bud cells. Furthermore, we found that P2Y_1-expressed cells coexpressed both IP3R_3 and SNAP-25 by double immunolabeling. These results suggest that ATP may activate P2Y_1 receptors resulting in Ca^<2+> release from internal stores via IP3R3. Since all SNAP-25 immunoreactive taste bud cells coexpressed P2Y_1 immunoreactive, it is suggested that P2Y_1 expressing cells may make synapses with afferent nerve fibers. In addition to P2Y_1 receptors, P2Y_4 receptors were expressed a subset of taste bud cells. We concluded that both P2Y and P2X receptors appear to be important regulators of taste bud functions.

细胞外核苷酸（例如 ATP）是与膜受体（P2X 配体门控离子通道和 G 蛋白偶联 P2Y 家族）结合的信号分子。在味觉系统中，P2X 受体（P2X_2 和 P2X_3）仅在支配味蕾的神经纤维中表达。此外，根据生理学研究，P2Y受体似乎在味蕾细胞的味觉信号转导中发挥一定作用。然而，P2Y受体亚型的表达模式尚未揭示。本研究通过RT PCR、免疫组织化学和原位杂交技术检测大鼠味觉乳头中P2Y_1受体亚型的表达模式。这些分析表明P2Y_1受体在味蕾细胞中表达。此外，我们通过双重免疫标记发现表达P2Y_1的细胞同时表达IP3R_3和SNAP-25。这些结果表明ATP可能激活P2Y_1受体，导致Ca^2+通过IP3R3从内部储存释放。由于所有 SNAP-25 免疫反应性味蕾细胞共表达 P2Y_1 免疫反应性，因此表明 P2Y_1 表达细胞可能与传入神经纤维形成突触。除了 P2Y_1 受体外，味蕾细胞的一个子集还表达 P2Y_4 受体。我们得出的结论是，P2Y 和 P2X 受体似乎都是味蕾功能的重要调节因子。