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Neurobiology on the development and regeneration of taste buds.

味蕾发育和再生的神经生物学。

基本信息

批准号：
11671805
负责人：
TOYOSHIMA Kuniaki
金额：
$ 2.37万
依托单位：
Kyushu Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

项目摘要

Taste-mGluR4, cloned from taste tissues, is a truncated variant of brain-expressed mGluR4a (brain-mGluR4), and is known to be a candidate for the receptor involved in the sense of umami taste. Although the expression patterns of taste- and brain-mGluR4 mRNAs have been demonstrated, no mention has so far been made of the expression of these two mGluR4 proteins in taste tissues. In order to examine the expression of taste-mGluR4 and brain-mGlu4 proteins in rat taste tissues, we used a specific antibody for mGuR4a, which shared C-terminus of both taste- and brain-mGluR4, for immunoblot analysis and immunohistochemistry.The immunoblot analysis showed that both brain-mGluR4 and taste-mGluR4 were expressed in the taste tissues. Taste-mGluR4 was not detected in the cerebellum. The immunoreactive band for brain-mGluR4 protein was much stronger than that for taste-mGluR4 protein. In the cryosections of fungiform, foliate and circumvallate papillae, the antibody against taste-mGluR4 gave intense labelling of the taste pores and taste hairs in all the taste buds of gustatory papillae examined ; the immunoreaction to the antibody against taste-mGluR4 was more intense at the same sites of taste buds. The portions of taste bud cells below the taste pore and surrounding keratinocytes did not show any immunoreactivities.The results of the present study strongly suggest that, in addition to taste-mGluR4 brain-mGluR4 may function, and even more importantly than the former, as a receptor for glutamate, i. e., umami, taste sensation.

taste - mglur4是从味觉组织中克隆出来的，是脑表达的mGluR4a （brain-mGluR4）的截断变体，已知是参与鲜味感觉的受体候选。虽然味觉和大脑mGluR4 mrna的表达模式已经被证实，但迄今为止还没有提到这两种mGluR4蛋白在味觉组织中的表达。为了检测taste- mglur4和brain-mGlu4蛋白在大鼠味觉组织中的表达，我们使用了mGuR4a的特异性抗体进行免疫印迹分析和免疫组织化学，该抗体共享味觉和脑- mglur4的c端。免疫印迹分析显示，脑- mglur4和味觉- mglur4在味觉组织中均有表达。小脑未检测到Taste-mGluR4。脑- mglur4蛋白的免疫反应带明显强于味觉- mglur4蛋白。在真菌状、叶状和环状乳头的冷冻切片中，抗taste- mglur4抗体对所检测的所有味觉乳头的味蕾中的味觉孔和味觉毛进行了强烈的标记；味蕾相同部位对taste- mglur4抗体的免疫反应更为强烈。味蕾细胞在味孔以下部分和周围的角质形成细胞未表现出任何免疫反应。本研究结果强烈提示，除了味觉- mglur4脑- mglur4可能作为谷氨酸，即鲜味，味觉的受体，甚至比前者更重要。