Cell death of primary sensory neurons in the trigeminal ganglion of Brn-3a knockout mice

Brn-3a 敲除小鼠三叉神经节初级感觉神经元细胞死亡

• 批准号: 14571733
• 负责人: ICHIKAWA Hiroyuki
• 金额: $ 1.98万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571733/
• 关键词: Brn-3a; Knockout mouse; Trigeminal ganglion; Sensory neuron; PGP 9.5; CGRP; VRL-1; CaBP; PGP9.5; calretinin; calbindin D-28k

Immunohistochemistry for parvalbumin, calbindin D-28k, calretinin and calcitonin gene-related peptide was performed on the trigeminal ganglion and oro-facial tissues in Brn-3a wildtype and knockout mice at embryonic day 18.5 and postnatal day 0. In wildtype mice, the trigeminal ganglion contained abundant parvalbumin-, calbindin D-28k- and calcitonin gene-related peptide-immunoreactive neurons while the ganglion was almost devoid of calretinin-immunoreactive neurons. In Brn-3a knockout mice, a 63% decrease of parvalbumin-immunoreactive neurons was detected. On the other hand, the absence of Brn-3a dramatically increased the number of calbindin D-28k- immunoreactive (3.5-fold increase) and calretinin-immunoreactive neurons (91-fold increase). The number of calcitonin gene-related peptide-immunoreactive neurons, however, was not altered by the Brn-3a deficiency. Cell size analysis indicated that loss of Brn-3a increased the proportions of sm all (<100 μm^2) parvalbumin-, calbindin D-28k- … More and calcitonin gene-related peptide-immunoreactive neurons while decreased those of large (>200 μm^2) immunoreactive cells. Calretinin-immunoreactive neurons were either small or medium (100-200 μm^2) in mutant mice. The oro-facial tissues contained parvalbumin-, calbindin D-28k- and calcitonin gene-related peptide-immunoreactive fibers but not calretinin-immunoreactive ones in wildtype mice. In Brn-3a knockout mice, the number of parvalbumin-immunoreactive fibers markedly decreased in the infraorbital nerve and parvalbumin-immunoreactive endings disappeared in the vibrissa. On the other hand, the number of calbindin D-28k-immunoreactive fibers increased significantly in the infraorbital and mental nerves. In addition, calbindin D-28k-immunoreactive endings appeared in the vibrissa. As well, some fibers showed calretinin-immunoreactivity In the infraorbital nerve of the mutant. However, no obvious charge of calcitonin gene-related peptide-immunoreactive fibers was observed in the oro-facial region of knockout mice. Taken together, these data suggest that Brn-3a deficiency has effects on the expression of neurochemical substances in the trigeminal ganglion. Less

在胚胎第 18.5 天和出生后第 0 天对 Brn-3a 野生型和敲除小鼠的三叉神经节和口腔面部组织进行小清蛋白、钙结合蛋白 D-28k、钙结合蛋白和降钙素基因相关肽的免疫组织化学分析。在野生型小鼠中，三叉神经节含有丰富的 小白蛋白、钙结合蛋白 D-28k 和降钙素基因相关肽免疫反应性神经元，而神经节几乎没有钙视网膜素免疫反应性神经元。在 Brn-3a 敲除小鼠中，检测到小清蛋白免疫反应性神经元减少了 63%。另一方面，Brn-3a 的缺失显着增加了钙结合蛋白 D-28k 免疫反应性神经元（增加 3.5 倍）和钙结合蛋白免疫反应性神经元（增加 91 倍）的数量。然而，降钙素基因相关肽免疫反应性神经元的数量并没有因 Brn-3a 缺陷而改变。细胞大小分析表明，Brn-3a 的缺失增加了小清蛋白 (<100 μm^2)、钙结合蛋白 D-28k-… 更多和降钙素基因相关肽免疫反应性神经元的比例，同时减少了大 (>200 μm^2) 免疫反应性细胞的比例。在突变小鼠中，钙结合蛋白免疫反应性神经元要么很小，要么中等（100-200 μm^2）。野生型小鼠的口腔面部组织含有小白蛋白、钙结合蛋白 D-28k 和降钙素基因相关的肽免疫反应性纤维，但不含有钙视网膜素免疫反应性纤维。在 Brn-3a 敲除小鼠中，眶下神经中小清蛋白免疫反应性纤维的数量显着减少，触须中小清蛋白免疫反应性末梢消失。另一方面，眶下神经和颏神经中钙结合蛋白 D-28k 免疫反应性纤维的数量显着增加。此外，触毛中还出现了钙结合蛋白 D-28k 免疫反应性末端。此外，突变体的眶下神经中的一些纤维显示出钙结合蛋白免疫反应性。然而，在基因敲除小鼠的口面部区域没有观察到降钙素基因相关肽免疫反应纤维的明显电荷。综上所述，这些数据表明 Brn-3a 缺乏对三叉神经节中神经化学物质的表达有影响。较少的

项目成果

Effect of Brn-3a deficiency on parvalbumin-, calbindin D-28k-, CR- and CGRP-IR primary sensory neurons in the trigeminal ganglion

Brn-3a 缺乏对三叉神经节小清蛋白、钙结合蛋白 D-28k-、CR- 和 CGRP-IR 初级感觉神经元的影响

• 发表时间: 2002
• 期刊: Neuroscience 113
• 作者: Ichikawa H et al.

Ichikawa et al.: "Calcium-binding protein-imnunoreactive innervation of the rat vibrissa"Brain Res.. 970・1-2. 226-231 (2003)

Ichikawa等：“大鼠触毛的钙结合蛋白-免疫反应性神经支配”Brain Res.. 970・1-2（2003）。

Effect of Brn-3a deficiency on parvalbumin-, calbindin D-28k-, calretinin- and calcitonin gene-related peptide-immunoreactive primary sensory neurons in the trigeminal ganglion

• DOI: 10.1016/s0306-4522(02)00182-3
• 发表时间: 2002-01-01
• 期刊: NEUROSCIENCE
• 影响因子: 3.3
• 作者: Ichikawa, H;Yamaai, T;Sugimoto, T
• 通讯作者: Sugimoto, T

Ichikawa et al.: "The co-expression of VR1 and VRL-1 in the rat vagal sensory ganglia"Brain Res.. 980・2. 293-296 (2003)

Ichikawa 等：“大鼠迷走神经感觉神经节中 VR1 和 VRL-1 的共表达”Brain Res.. 980・2 (2003)。

Effect of Brn-3a deficiency on parvalbumin-immunoreactive primary sensory neurons in the dorsal root ganglion

• DOI: 10.1016/j.devbrainres.2004.02.004
• 发表时间: 2004-05-19
• 期刊: DEVELOPMENTAL BRAIN RESEARCH
• 作者: Ichikawa, H;Mo, Z;Sugimoto, T
• 通讯作者: Sugimoto, T