The relationship between neural substances (calcium-binding proteins and neuropeptides) and inflammation in the tooth pulp and salivary gland

大鼠三叉神经节含有丰富的钙结合蛋白D-28K(CB)或S100-ir免疫反应(Ir)神经元。这些神经元大多很大，分布在整个神经节中。双重免疫荧光法显示钙结合蛋白(CaBPs)、钙视黄素(CR)和小白蛋白(PV)共存。在大鼠磨牙牙髓内可见CB或S100免疫反应神经纤维。S100-ir定位于雪旺细胞、无髓轴突和有髓轴突，而CB-ir定位于有髓轴突。逆行追踪和免疫组织化学方法证实CB或S100免疫反应阳性神经纤维起源于三叉神经节。分别有23%和50%的牙髓初级神经元表达CB和S100。向下颌下神经管注射10微升的完全弗氏佐剂(CFA)后，观察CABP或神经肽免疫反应神经纤维分布的变化。在正常下颌管周围神经纤维中可见CR-、P物质(SP)-OR和降钙素基因相关肽(CGRP)-ir。CR-ir神经纤维共表达SP-和CGRP-IR。注射CFA 5天后，炎症的颌下神经管周围CR-ir消失，而CGRP和SP-ir的表达无变化。石蜡切片中含有CRmRNA的信号，而固定切片和新鲜冰冻切片中则没有。这些信号多见于大神经元。这与先前CR的免疫组织化学数据一致。

The rat trigeminal ganglion contained abundant calbindin D-28k (CB) - or S100-ir immunorecative (ir) neurons. These neurons are mostly large and distributed throughout the ganglion. The double immunofluorescence method revealed the coexistence of these calcium-binding proteins (CaBPs), calretinin (CR) and parvalbumin (PV). CB-or S100-ir nerve fibers were observed in the molar tooth pulp of the rat. S100-ir was localized to Schwann cells, and unmyelinated and myelinated axons, whereas CB-ir was to myelinated axons. The retrograde tracing and immunohistochemical methods demonstrated that CB- or S100-ir nerve fibers originated from the trigeminal ganglion. 23 % and 50 % of tooth pulp primary neurons were immunoreactive for CB and S100, respectively.The change in the distirution of CaBP- or neuropeptide-ir nerve fibers was examined after administration of 10 micro 1 of complete freund adjuvant (CFA) to the submandibular ducts. CR-, substance P (SP) - or and calcitonin gene-related peptide (CGRP) -ir was observed in nerve fibers around the normal submandibular duct. The CR-ir nerve fibers coexpressed SP- and CGRP-irs. 5 days after the injection of CFA,CR-ir disappeared around the inflamed submandibular duct, while CGRP- or SP-ir ones remained unchanged.CR mRNA was examined in paraffin, fixed frozen and fresh frozen sections of the trigeminal ganglion. The paraffin sections contained the signals for CR mRNA,while fixed or fresh frozen sections were devoid of them. The signal were mostly observed in large neurons. This was consistent with the previous immunohistochemical data for CR.