The relationship between neural substances (calcium-binding proteins and neuropeptides) and inflammation in the tooth pulp and salivary gland

神经物质（钙结合蛋白和神经肽）与牙髓和唾液腺炎症的关系

• 批准号: 07671971
• 负责人: ICHIKAWA Hiroyuki
• 金额: $ 1.41万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671971/
• 关键词: Calcium-binding proteins; Rat; Submandibular ganglion; Tooth pulp; Trigeminal ganglion; 免疫組織化学; in situ hybridization; 歯髄; 唾液腺; カルレチニン; パルブアルブミン; カルビンデイン; 口蓋; 歯根膜

The rat trigeminal ganglion contained abundant calbindin D-28k (CB) - or S100-ir immunorecative (ir) neurons. These neurons are mostly large and distributed throughout the ganglion. The double immunofluorescence method revealed the coexistence of these calcium-binding proteins (CaBPs), calretinin (CR) and parvalbumin (PV). CB-or S100-ir nerve fibers were observed in the molar tooth pulp of the rat. S100-ir was localized to Schwann cells, and unmyelinated and myelinated axons, whereas CB-ir was to myelinated axons. The retrograde tracing and immunohistochemical methods demonstrated that CB- or S100-ir nerve fibers originated from the trigeminal ganglion. 23 % and 50 % of tooth pulp primary neurons were immunoreactive for CB and S100, respectively.The change in the distirution of CaBP- or neuropeptide-ir nerve fibers was examined after administration of 10 micro 1 of complete freund adjuvant (CFA) to the submandibular ducts. CR-, substance P (SP) - or and calcitonin gene-related peptide (CGRP) -ir was observed in nerve fibers around the normal submandibular duct. The CR-ir nerve fibers coexpressed SP- and CGRP-irs. 5 days after the injection of CFA,CR-ir disappeared around the inflamed submandibular duct, while CGRP- or SP-ir ones remained unchanged.CR mRNA was examined in paraffin, fixed frozen and fresh frozen sections of the trigeminal ganglion. The paraffin sections contained the signals for CR mRNA,while fixed or fresh frozen sections were devoid of them. The signal were mostly observed in large neurons. This was consistent with the previous immunohistochemical data for CR.

大鼠三叉神经节内含有丰富的钙结合蛋白D-28 k（CB）或S100免疫反应（ir）神经元。这些神经元大多数很大，分布在整个神经节。免疫荧光双标法显示这些钙结合蛋白（CaBPs）、钙视网膜蛋白（CR）和小清蛋白（PV）共存。在大鼠磨牙牙髓中观察到CB或S100阳性神经纤维。S100-ir定位于雪旺细胞、无髓和有髓轴突，而CB-ir定位于有髓轴突。逆行追踪和免疫组织化学方法证实CB和S100阳性神经纤维均起源于三叉神经节。CB和S_（100）免疫反应阳性神经元分别为23%和50%。正常颌下腺导管周围神经纤维中可见CR阳性、P物质（SP）阳性和降钙素基因相关肽（CGRP）阳性。CR-ir神经纤维共表达SP-和CGRP-ir。注射CFA后5d，炎症的颌下腺导管周围CR-ir消失，CGRP-ir和SP-ir无明显变化。石蜡切片含有CR mRNA的信号，而固定或新鲜冷冻切片则没有。信号主要在大神经元中观察到。这与之前CR的免疫组化数据一致。

项目成果

H.Ichikawa et al.: "Parvalbumin-immunoreactive nerve endings in the periodontal ligumont of rat teath" Archives of oral Biology. (in press).

H.Ich​​ikawa 等人：“大鼠牙齿牙周膜中的小清蛋白免疫反应性神经末梢”口腔生物学档案。

H.Ichikawa et al.: "Calbinding-D28k-immunoreactivity in the trigeminal ganglion and molar tooth pulp of the rat" Brain Resaerch. 71-78 (1996)

H.Ich​​ikawa 等人：“大鼠三叉神经节和磨牙牙髓中的钙结合-D28k-免疫反应性”Brain Resaerch。

H.Ichikawa et al.: "calbindin-D28K-immunoreactivity in the trigeminal garglion neurons and molar tooth pulp of the rat" Brain Research. 715. 71-78 (1996)

H.Ich​​ikawa 等人：“大鼠三叉神经元和磨牙牙髓中的钙结合蛋白-D28K-免疫反应性”大脑研究。

H.Ichikawa et al.: "Calbindin-D28k-immunoreactivity in the trigeminal ganglion newos and molar tooth pulp of the rat" Brain Research. 715. 71-78 (1996)

H.Ich​​ikawa 等人：“大鼠三叉神经节 newos 和磨牙牙髓中的 Calbindin-D28k-免疫反应性”大脑研究。

H.Ichikawa: "Parvalbumin-and calretinin-immunoreactive trigeminal neurons innervating the rat molar tooth pulp." Brain Research. 679. 205-211 (1995)

H.Ich​​ikawa：“小白蛋白和钙结合蛋白免疫反应性三叉神经元支配大鼠磨牙牙髓。”