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Basic Research for the role and mechanism of histamine in visual procession

组胺在视觉过程中的作用及机制的基础研究

基本信息

批准号：
11671721
负责人：
NAKAGAWA Yoichi
金额：
$ 2.43万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11671721/
关键词：
Histamine H3 receptor Visual Processing

项目摘要

We investigated the changes of histamine receptors in the brains of rats after unilateral enucleation and monocular deprivation using quantitative autoradiography. The H3 receptor binding in the visually deprived superior colliculus (SO. Contralateral to the enucleated eye, was significantly increased 5, 15, 30 and 45 days after unilateral cnuclcation. Continuous injection of (S)-alpha-fluoronielhylhistidine. A specific inhibitor of l.-hislidine decarboxylase, and local injection of quinolinic acid attenuated the effect of unilateral enucleation in the SC. In infant rats, the H3 receptor binding in the visually deprived superior colliculus was significant!} increased after unilateral enucleation. Hut there were no effects of monocular deprivation on the receptor bindings. No significant changes in H1 and H2 receptor bindings were observed in the SC after unilateral enucleation and monocular deprivation. The [^<35>S]GTPγS-binding response was significantly increased in the contralateral SC in which H3 receptor binding response was enhanced. The increased [^<35>S]GTPγS signal was reversed with H_3-selective antagonists, whereas specific H_1 and H_2 antagonists were proved ineffective.These results suggest that retinal differentiation induced an increase in histamine H3 receptor binding sites, probably by selective adjustment of hislamine neurons in response to unilateral enucleation, and the tip-regulated H3 receptors caused by denervation were coupled with G proteins.

我们使用定量放射自显影技术研究了单侧剜除和单眼剥夺后大鼠大脑中组胺受体的变化。视觉剥夺的上丘（SO。去核眼的对侧，在单侧去核后 5、15、30 和 45 天显着增加。连续注射 (S)-α-氟代组氨酸。L.-组氨酸脱羧酶的特异性抑制剂，局部注射喹啉酸减弱了 SC 的单侧剜除术。在幼年大鼠中，单侧摘除后，视觉剥夺的上丘中的 H3 受体结合显着增加！}但单眼剥夺对受体结合没有影响。单侧摘除和单眼剥夺后，SC 中 H1 和 H2 受体结合没有观察到显着变化。的 [^ 35 S]GTPγS结合反应在对侧SC中显着增加，其中H3受体结合反应增强。 H_3选择性拮抗剂可逆转增加的[^ 35 S]GTPγS信号，而特异性H_1和H_2拮抗剂被证明无效。这些结果表明视网膜分化诱导组胺H3受体结合增加可能是通过选择性调整组胺神经元来响应单侧剜除，并且由去神经引起的尖端调节的 H3 受体与 G 蛋白偶联。