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Microbiological study on oral bacterial floral shifts in children

儿童口腔细菌花移的微生物学研究

基本信息

批准号：
14571944
负责人：
MATSUYAMA Junko
金额：
$ 2.18万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571944/
关键词：
PCR PCR-RFLP children microflora dental caries periodontitis 口腔細菌叢ミュータンスレンサ球菌

项目摘要

Mutans streptococci have been implicated as cariogenic bacteria in the oral cavity. It has been reported that the diversity of detection frequencies of mutans streptococci from human dental plaque, and these diversity may be due to the differences of subject age and/or target genes tested. In this study, detection of mutans streptococci from human dental plaque were performed with polymerase chain reaction(PCR) using primers base upon 16S ribosomal RNA, glucosyltransferase(gft) and dextranase(dex) genes, and their detection frequencies were compared. Detection frequencies of mutans streptococci by PCR with primers based upon the 16S rRNA genes were higher than those based upon the gtf and dex genes from human dental plaque of children. Utilizing PCR with primers based upon the 16S rRNA genes, S.mutans were detected from all of the samples tested in this study. On the other hand, the detection frequencies of S.sobrinus were lower (10%) than those of S.mutans, and S.sobrinus were likely detected in the subjects of deciduous attained-dentition and mixed-dentition stage. The 16S rRNA gene-based nested PCR method is a rapid and sensitive method for the detection of mutans streptococci, and may also be suitable for carrying out large-scale studies on the cariogenicity of mutans streptococci. Furthermore, this method can potentially detect not only mutans streptococci but also other oral bacterial species, such as periodontopathic bacteria, by substituting specific primers for each oral bacterial species during the second PCR.

变形链球菌被认为是口腔中的致龋细菌。据报道，人类牙菌斑中变形链球菌的检测频率存在多样性，这些多样性可能是由于受试者年龄和/或检测目标基因的差异。本研究以16S核糖体RNA、葡萄糖基转移酶（gft）和葡聚糖酶（dex）基因为引物，采用聚合酶链式反应（PCR）检测人牙菌斑中的变形链球菌，并比较它们的检出率。以16S rRNA基因为引物的PCR对突变链球菌的检出率高于以儿童人牙菌斑gtf和dex基因为引物的检出率。采用基于16S rRNA基因的引物PCR方法，在本研究的所有样品中检测到变形链球菌。另一方面，sobrinus的检出率低于s.a mutans(10%)，并且sobrinus可能在乳牙发育期和混合牙列期的受试者中检测到。基于16S rRNA基因的巢式PCR方法是一种快速、灵敏的突变链球菌检测方法，也可能适合开展突变链球菌致病性的大规模研究。此外，该方法不仅可以检测变形链球菌，还可以通过在第二次PCR中为每个口腔细菌物种替换特定引物来检测其他口腔细菌物种，如牙周病细菌。