Reproductive functional expression by the xenogenic gonad transplantation

用2.5Gy射线照射SCID小鼠，将新生仓鼠的卵巢移植到SCID小鼠的卵巢内。或摘除SCID小鼠卵巢，离开卵巢系膜，将仓鼠卵巢移植到卵巢系膜。从一周后卵巢移植后的阴道涂片检查中可以证实，这些小鼠出现了动情周期。然后，当它与切除输精管的雄鼠交配时，阴道塞在大约2个月的时间里能够确认大约70%的小鼠。在阴道塞子的小鼠宫腔内，注射从仓鼠附睾处采集的精子。结果，有20%的小鼠能够确认怀孕，但这些小鼠不能生育。将大鼠的胸腺雏形移植到裸鼠的肾被膜下时，只取胸腺上皮。宿主小鼠的树突状细胞和前体T细胞聚集在胸腺，T细胞免疫很快就是一种…更需要(TG裸鼠)。GVH病和系统性红斑狼疮样自身免疫性疾病在TG裸鼠身上不出现。TG裸鼠终生接受胸腺供体皮肤，但排斥第三方皮肤。这表明，TG裸鼠的免疫系统认为胸腺供体为自身。然而，器官局限性自身免疫性疾病的发生在转基因裸鼠的许多器官中都可以看到。在转基因裸鼠身上，这种自身免疫性疾病在卵巢上也是高发的。将新生小鼠卵巢移植到Tg裸鼠肾被膜下，卵巢破坏严重，淋巴细胞大量浸润。然而，将胸腺供体的卵巢移植到TG裸鼠体内，卵巢生长正常，没有任何炎症反应。说明不同种类的供体卵巢在转基因裸鼠体内均可获得功能性表达。将异种动物的胸腺和卵巢移植给裸鼠，并检测小鼠的生育力。较少

Ovaries of neonate Dzuagarian hamster were transplanted in the ovaries of scid mice which irradiated the x-lay of 2.5 Gy. Or ovaries of scid mice were extracted leaving mesovarium and transplanted hamster ovaries in mesovarium. It was able to be confirmed that the estrous cycle appeared in the inspection of vagina smear of these mice after ovary transplant from post-one week. Then, the vagina plug was able to confirm to about 70% mice during about 2 months, when it was made to mate with the male mouse which removed vas deferens. In the intrauterine of the mouse with the vagina plug, the sperm collected from the epididymis of the hamster was injected. As a result, 20% of mice were able to confirm the pregnancy, but these mice were not able to give birth.When the thymic rudiment of the rat is transplanted under the renal capsule of nude mouse, only the thymic epithelium is taken. Dendritic cell and precursor T cell of the host mouse accumulate in the thymus, and T cell immunity is soon a … More cquired (TG nude mouse). The GVH disease and SLE-like systemic autoimmune disease does not appear on TG nude mice. TG nude mice receive skin of thymic donor throughout the life, but reject skin of the third party. This shows that immune system of TG nude mice considers a thymus donor to be self. However, development of organ localized autoimmune diseases is seen in the many organs of TG nude mice. In TG nude mice, the autoimmune disease also appears high-frequent on the ovary. When newborn mouse ovary is transplanted under the renal capsule of TG nude mice, severe destruction of the ovary with massive lymphocyte infiltration was observed. However, when the ovary of the thymic donor was transplanted to the TG nude mice, the ovary grows normally without any inflammatory reaction. These show that functional expression of the different kind donor ovary can reappear in TG nude mice. Transplantation of thymus and ovary of xenogenic animals will be tried to the nude mice, and the fertility of the mice will be examined. Less