Reproductive functional expression by the xenogenic gonad transplantation

采用2.5 Gy的x射线照射，将幼鼠的卵巢移植到小鼠卵巢中。将小鼠卵巢从肠系膜中取出，将仓鼠卵巢移植到肠系膜中。卵巢移植后1周开始阴道涂片检查，可以证实出现了发情周期。然后，在大约两个月的时间里，阴道塞能够与70%的老鼠确认，当它与切除输精管的雄性老鼠交配时。在阴道堵塞的小鼠子宫内，从仓鼠的附睾收集的精子被注射。结果，20%的小鼠能够确认怀孕，但这些小鼠无法分娩。将大鼠胸腺雏形移植到裸鼠肾包膜下，只取胸腺上皮。宿主小鼠的树突状细胞和前体T细胞在胸腺内积累，很快获得T细胞免疫（TG裸小鼠）。甘油三酯裸鼠未出现GVH疾病和slea样全身自身免疫性疾病。TG裸鼠终生接受胸腺供体皮肤，排斥第三方皮肤。这说明TG裸鼠免疫系统认为胸腺供体是自我的。然而，在TG裸鼠的许多器官中可以看到器官局限性自身免疫性疾病的发展。在TG裸小鼠中，自身免疫性疾病也出现在卵巢上。将新生小鼠卵巢移植到TG裸鼠肾包膜下，观察到卵巢严重破坏，淋巴细胞大量浸润。而将胸腺供体卵巢移植到TG裸鼠体内后，卵巢生长正常，无任何炎症反应。说明TG裸鼠可重现不同类型供体卵巢的功能性表达。尝试将异种动物的胸腺和卵巢移植到裸鼠身上，并检查小鼠的生育能力。少

Ovaries of neonate Dzuagarian hamster were transplanted in the ovaries of scid mice which irradiated the x-lay of 2.5 Gy. Or ovaries of scid mice were extracted leaving mesovarium and transplanted hamster ovaries in mesovarium. It was able to be confirmed that the estrous cycle appeared in the inspection of vagina smear of these mice after ovary transplant from post-one week. Then, the vagina plug was able to confirm to about 70% mice during about 2 months, when it was made to mate with the male mouse which removed vas deferens. In the intrauterine of the mouse with the vagina plug, the sperm collected from the epididymis of the hamster was injected. As a result, 20% of mice were able to confirm the pregnancy, but these mice were not able to give birth.When the thymic rudiment of the rat is transplanted under the renal capsule of nude mouse, only the thymic epithelium is taken. Dendritic cell and precursor T cell of the host mouse accumulate in the thymus, and T cell immunity is soon a … More cquired (TG nude mouse). The GVH disease and SLE-like systemic autoimmune disease does not appear on TG nude mice. TG nude mice receive skin of thymic donor throughout the life, but reject skin of the third party. This shows that immune system of TG nude mice considers a thymus donor to be self. However, development of organ localized autoimmune diseases is seen in the many organs of TG nude mice. In TG nude mice, the autoimmune disease also appears high-frequent on the ovary. When newborn mouse ovary is transplanted under the renal capsule of TG nude mice, severe destruction of the ovary with massive lymphocyte infiltration was observed. However, when the ovary of the thymic donor was transplanted to the TG nude mice, the ovary grows normally without any inflammatory reaction. These show that functional expression of the different kind donor ovary can reappear in TG nude mice. Transplantation of thymus and ovary of xenogenic animals will be tried to the nude mice, and the fertility of the mice will be examined. Less