Reproductive functional expression by the xenogenic gonad transplantation

将新生达氏仓鼠卵巢移植到经2.5戈伊照射的scid小鼠卵巢内。或将scid小鼠卵巢取出，保留系膜，将仓鼠卵巢移植于系膜。从卵巢移植后一周开始，这些小鼠的阴道涂片检查能够证实出现了发情周期。然后，阴道塞能够在约2个月期间对约70%的小鼠进行确认，当其与去除输精管的雄性小鼠交配时。将从仓鼠附睾收集的精子注射到带阴道栓的小鼠子宫内。结果，20%的小鼠能够确认妊娠，但这些小鼠不能生育。当将大鼠的胸腺原基移植到裸鼠的肾包膜下时，仅取胸腺上皮。宿主小鼠的树突状细胞和前体T细胞在胸腺中积聚，T细胞免疫很快被破坏， ...更多信息 获得（TG裸鼠）。TG裸鼠未出现GVH病和SLE样系统性自身免疫病。TG裸鼠终生接受胸腺供体皮肤，排斥第三方皮肤。这表明TG裸鼠的免疫系统将胸腺供体视为自身。然而，在TG裸鼠的许多器官中观察到器官局部自身免疫性疾病的发展。在TG裸鼠中，自身免疫性疾病也在卵巢上出现频率较高。将新生小鼠卵巢移植于TG裸鼠肾包膜下，可观察到卵巢严重破坏，并伴有大量淋巴细胞浸润。然而，当胸腺供体的卵巢移植到TG裸鼠时，卵巢正常生长，没有任何炎症反应。表明不同供者卵巢在TG裸鼠体内均能恢复功能表达。将异种动物的胸腺和卵巢尝试移植到裸鼠身上，并检查小鼠的生育能力。少

Ovaries of neonate Dzuagarian hamster were transplanted in the ovaries of scid mice which irradiated the x-lay of 2.5 Gy. Or ovaries of scid mice were extracted leaving mesovarium and transplanted hamster ovaries in mesovarium. It was able to be confirmed that the estrous cycle appeared in the inspection of vagina smear of these mice after ovary transplant from post-one week. Then, the vagina plug was able to confirm to about 70% mice during about 2 months, when it was made to mate with the male mouse which removed vas deferens. In the intrauterine of the mouse with the vagina plug, the sperm collected from the epididymis of the hamster was injected. As a result, 20% of mice were able to confirm the pregnancy, but these mice were not able to give birth.When the thymic rudiment of the rat is transplanted under the renal capsule of nude mouse, only the thymic epithelium is taken. Dendritic cell and precursor T cell of the host mouse accumulate in the thymus, and T cell immunity is soon a … More cquired (TG nude mouse). The GVH disease and SLE-like systemic autoimmune disease does not appear on TG nude mice. TG nude mice receive skin of thymic donor throughout the life, but reject skin of the third party. This shows that immune system of TG nude mice considers a thymus donor to be self. However, development of organ localized autoimmune diseases is seen in the many organs of TG nude mice. In TG nude mice, the autoimmune disease also appears high-frequent on the ovary. When newborn mouse ovary is transplanted under the renal capsule of TG nude mice, severe destruction of the ovary with massive lymphocyte infiltration was observed. However, when the ovary of the thymic donor was transplanted to the TG nude mice, the ovary grows normally without any inflammatory reaction. These show that functional expression of the different kind donor ovary can reappear in TG nude mice. Transplantation of thymus and ovary of xenogenic animals will be tried to the nude mice, and the fertility of the mice will be examined. Less