Possibility of disease prevention by regulating fatty acid nutrition

通过调节脂肪酸营养来预防疾病的可能性

基本信息

  • 批准号:
    16500511
  • 负责人:
  • 金额:
    $ 2.37万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2005
  • 项目状态:
    已结题

项目摘要

Tumor necrosis factor (TNF) is a major pro-inflammatory cytokine with a wide variety of functions, one of which is induction of cell death including apoptosis and necrosis. In this study, we explored effects of docosahexaenoic acid (DHA) and other polyunsaturated fatty acids (PUFAs) on TNF-induced cell death.The effect of preincubation with DHA on cell death was examined using various cultured cells, and compared with the cases of other fatty acids supplementation. Preincubation of cells with DHA for 24 h enriched DHA in cellular phospholipids. DHA showed the distinct ability to reduce TNF-induced apoptosis of U937 cells, but this was not the case in the supplementation of eicosapentaenoic acid (EPA) and arachidonic acid (AA). The inhibition of apoptosis was accompanied by enrichment of DHA in membrane phospholipids, indicating that DHA exerted its inhibitory activity after being incorporated into the phospholipids.In L929 cells, TNF can induce necrosis, another mode of cell death, and DHA treatment could also reduce this type of cell death. Unlike the case of U937 cell apoptosis, AA significantly attenuated L929 cell necrosis, and 20:3(n-6) or 22:4(n-6) showed similar or less activity, respectively. The order of effective PUFA activity was DHA>docosapentaenoic acid (n-3, DPA)【greater than or equal】EPA>AA【approximately equal】20:3(n-6)【greater than or equal】22:4(n-6). Fatty acid analysis of membrane phospholipids suggested that attenuation of TNF-induced necrosis is mainly attributable to the enrichment of three kinds of PUFAs, i.e. DHA, DPAn-3 or AA, in phospholipids. Among these fatty acids, DHA was the most effective in the reduction of L929 necrosis as observed in the case of U937 apoptosis.In summary, DHA-enriched membranes can protect cell irrespective of death modes and membranous DHA may abrogate the death signaling common to necrosis and apoptosis.
肿瘤坏死因子(TNF)是一种主要的促炎细胞因子,具有多种功能,其中之一是诱导细胞死亡,包括凋亡和坏死。在本研究中,我们探讨了二十二碳六烯酸(DHA)和其他多不饱和脂肪酸(PUFAs)对TNF诱导的细胞死亡的影响。使用各种培养细胞检测了DHA预孵育对细胞死亡的影响,并与补充其他脂肪酸的情况进行了比较。用DHA预孵育细胞24小时,使细胞磷脂中的DHA富集。DHA显示出明显的减少TNF诱导的U937细胞凋亡的能力,但在补充二十碳五烯酸(EPA)和花生四烯酸(AA)的情况下并非如此。在L929细胞中,TNF可诱导另一种细胞死亡方式--坏死,DHA处理也可减少这种细胞死亡。与U937细胞凋亡的情况不同,AA显著减弱L929细胞坏死,并且20:3(n-6)或22:4(n-6)分别显示类似或更少的活性。有效PUFA活性的顺序为DHA>二十二碳五烯酸(n-3,DPA)[大于或等于]EPA>AA[约等于]20:3(n-6)[大于或等于]22:4(n-6)。膜磷脂的脂肪酸分析表明,TNF诱导的坏死的衰减主要归因于三种PUFAs,即DHA,DPAn-3或AA,在磷脂中的富集。在这些脂肪酸中,DHA在减少L929坏死方面是最有效的,正如在U937凋亡的情况下所观察到的那样。总之,富含DHA的膜可以保护细胞,而不管死亡模式如何,膜DHA可以消除坏死和凋亡共同的死亡信号。

项目成果

期刊论文数量(18)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Effects of enrichment of L929 cell membrane with polyunsaturated fatty acids on tumor necrosis factor-induced cell death
多不饱和脂肪酸富集L929细胞膜对肿瘤坏死因子诱导的细胞死亡的影响
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    DING;Yuanying et al.;Tsukiyama K;Kubo M.K.;Etsu Kishida
  • 通讯作者:
    Etsu Kishida
Docosahexaenoic acid enrichment of membrane phospholipid can reduce L929 cell necrosis induced by tumor necrosis factor
二十二碳六烯酸富集膜磷脂可减少肿瘤坏死因子诱导的L929细胞坏死
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sumiko Odani;Atsushi Suzuki;Takashi Hara;Etsu Kishida
  • 通讯作者:
    Etsu Kishida
日本人の脂質摂取基準2005の「わかりにくさ」と検討すべき課題
“难以理解”的2005年日本脂肪摄入标准和需要考虑的问题
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Sumiko Odani;Atsushi Suzuki;Takashi Hara;Etsu Kishida;Yasuo Masuzawa;増澤康男
  • 通讯作者:
    増澤康男
調理モデル系におけるアスコルビン酸の安定性に及ぼす油脂の影響
烹饪模型系统中脂肪和油对抗坏血酸稳定性的影响
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
    久保加織;西恭兵;堀越昌子;小川 和宏 ら;岸田恵津
  • 通讯作者:
    岸田恵津
Docosahexaenoic acid enrichment can reduce L929 cell necrosis induced by tumor necrosis factor
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KISHIDA Etsu其他文献

KISHIDA Etsu的其他文献

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{{ truncateString('KISHIDA Etsu', 18)}}的其他基金

Palatability and nutrient retention of steamed vegetables
蒸蔬菜的适口性和营养保留
  • 批准号:
    18K02239
  • 财政年份:
    2018
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Practice and evaluation of Shokuiku related specifically to course subject learning: Proposal of an instruction model using a subject-related, subject-integrated approach
与课程科目学习相关的食育实践和评估:提出采用科目相关、科目综合方法的教学模式
  • 批准号:
    15K04497
  • 财政年份:
    2015
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Establishment, Practical Application and Evaluation of the Consistent Nutrition Education Curriculum through Primary and Secondary Education with the Aim of Developing an Appropriate Lifestyle
以养成适当的生活方式为目标的中小学教育一致性营养教育课程的建立、实践应用和评估
  • 批准号:
    24531200
  • 财政年份:
    2012
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
New function of antioxidants in foods
食品中抗氧化剂的新功能
  • 批准号:
    13680150
  • 财政年份:
    2001
  • 资助金额:
    $ 2.37万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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炎性反应中巨噬细胞激活诱导死亡(activation-induced cell death,AICD)的机理研究
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