Clarification of cause wheat protein in exercise-induced anaphylaxis with respect to wheat processed food

将小麦蛋白分为盐溶性、乙醇溶性和碱溶性蛋白，以明确小麦依赖性运动过敏反应的主要过敏原。通过使用大鼠制备针对每种蛋白质的特异性抗血清。胃蛋白酶对盐溶性蛋白质的消化率高于其它分级蛋白质。在给ICR小鼠施用每种分级蛋白质并进行负荷跑台运动30分钟后，测量残留在消化道中的蛋白质。在小肠可溶性内容物中检测到各组分蛋白保持了抗原性。此外，在每个门静脉血中检测到每种蛋白质保持抗原性。B10.在口服摄入每种蛋白质后，用每种分级蛋白质致敏的小鼠在跑步机上跑步。力竭运动后致敏小鼠肠粘膜糜烂程度均高于未致敏小鼠。此外，适度运动30分钟后， ...更多信息 每种小麦分级蛋白的口服摄入也诱导麦醇溶蛋白和麦谷蛋白组的肠侵蚀。此外，我们还观察到麦醇溶蛋白和麦谷蛋白从肠道泄漏到肝脏。这些结果表明，小麦运动过敏反应的主要致敏因子可能是小麦蛋白质中的醇溶蛋白和谷蛋白，醇溶蛋白是小麦运动过敏反应的主要过敏原，它不溶于盐溶液，而食物过敏原一般是可溶性的。结果表明，不溶性醇溶蛋白在胆汁乳化条件下被蛋白酶消化，并被顺利吸收，保持了抗原活性。经口给予未致敏小鼠各分级蛋白后，检测各蛋白的消化吸收率。经口给予麦醇溶蛋白后，小鼠胃肠道粘膜损伤明显，H-E染色可见小肠绒毛脱落。根据这一结果，提示麦胶蛋白消化产生的某些肽与乳糜泻的致病肽相同，对小肠粘膜有毒性作用。此外，演习使损害更加严重。少

The wheat protein was fractionated in the salt soluble, EtOH soluble and alkali soluble proteins to clarify the main allergen concerning the wheat dependent exercise-induced anaphylaxis. Specific antisera to each protein was prepared by using the rat. The digestibility of the salt soluble protein with the pepsin advanced comparing the other fractionated proteins. After ICR mouse was administered each fractionated protein and loaded treadmill running for 30 min, the protein remained in a digestive canal was measured. Each fractionated protein maintained the antigenicity was detected in the soluble content of small intestines. In addition, each protein maintained antigenicity was detected in each portal blood. B10.A mouse sensitized with each fractionated protein was run on a treadmill after oral intake each protein. The level of intestinal erosion was higher in all sensitized mice than that in the unsensitized ones after exhaustive running. Further more, moderate exercise for 30 min aft … More er oral ingestion of each wheat-fractionated protein also induced intestinal erosion in the gliadin- and glutenin-groups. In addition, we observed leaking of gliadin and glutenin proteins out of the intestine into the liver. These results indicated that the main factor involved in wheat-dependent exercise-induced anaphylaxis might be the gliadin and glutenin in wheat proteins.Gliadin, a major allergen of wheat-dependent exercise-induced anaphylaxis, is insoluble in a salt solution, though food allergens are generally soluble. It was suggested that insoluble gliadin was digested by protease under the emulsified condition of bile and smoothly absorbed holding the antigenic activity. After the orally administrate of each fractionated protein to unsensitized mouse, the digestibility and absorption of each protein were examined. The gastrointestinal mucosal lesion was remarkably observed in the mouse oral administrated gliadin and remarkable dropout of the villus of the mouse small intestine was admitted by the H-E dye. As for this result, it is suggested that some peptide generated by gliadin digestion identical with a cause peptide of the celiac disease to have the toxicity action on the small intestines mucous. Moreover, the exercise made the damage more seriously. Less