Clarification of cause wheat protein in exercise-induced anaphylaxis with respect to wheat processed food

澄清小麦加工食品中运动引起的过敏反应的原因小麦蛋白

• 批准号: 16500527
• 负责人: KATO Yasuko
• 金额: $ 2.3万
• 依托单位: Kawasaki University of Medical Welfare
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16500527/
• 关键词: wheat protein; exercise-induced anaphylaxis; gliadin; gastrointestinal mucosa; digestibility; 運動誘発アナフェラキシー; 小腸粘膜上皮組織; グルテニン; ペプシン消化; 消化管内消化

The wheat protein was fractionated in the salt soluble, EtOH soluble and alkali soluble proteins to clarify the main allergen concerning the wheat dependent exercise-induced anaphylaxis. Specific antisera to each protein was prepared by using the rat. The digestibility of the salt soluble protein with the pepsin advanced comparing the other fractionated proteins. After ICR mouse was administered each fractionated protein and loaded treadmill running for 30 min, the protein remained in a digestive canal was measured. Each fractionated protein maintained the antigenicity was detected in the soluble content of small intestines. In addition, each protein maintained antigenicity was detected in each portal blood. B10.A mouse sensitized with each fractionated protein was run on a treadmill after oral intake each protein. The level of intestinal erosion was higher in all sensitized mice than that in the unsensitized ones after exhaustive running. Further more, moderate exercise for 30 min aft … More er oral ingestion of each wheat-fractionated protein also induced intestinal erosion in the gliadin- and glutenin-groups. In addition, we observed leaking of gliadin and glutenin proteins out of the intestine into the liver. These results indicated that the main factor involved in wheat-dependent exercise-induced anaphylaxis might be the gliadin and glutenin in wheat proteins.Gliadin, a major allergen of wheat-dependent exercise-induced anaphylaxis, is insoluble in a salt solution, though food allergens are generally soluble. It was suggested that insoluble gliadin was digested by protease under the emulsified condition of bile and smoothly absorbed holding the antigenic activity. After the orally administrate of each fractionated protein to unsensitized mouse, the digestibility and absorption of each protein were examined. The gastrointestinal mucosal lesion was remarkably observed in the mouse oral administrated gliadin and remarkable dropout of the villus of the mouse small intestine was admitted by the H-E dye. As for this result, it is suggested that some peptide generated by gliadin digestion identical with a cause peptide of the celiac disease to have the toxicity action on the small intestines mucous. Moreover, the exercise made the damage more seriously. Less

对小麦蛋白进行盐溶性、乙醇溶性和碱溶性蛋白的分离，以明确小麦依赖性运动致过敏反应的主要过敏原。利用大鼠制备了针对每种蛋白的特异性抗血清。盐溶性蛋白与胃蛋白酶的消化率比其他分离蛋白高。给ICR小鼠每一种分离蛋白并加载跑步机30分钟后，测量在消化道中保留的蛋白质。各分离蛋白在小肠可溶性含量中均保持了抗原性。此外，在每个门静脉血液中检测到每种维持抗原性的蛋白。B10。对每种分离蛋白质致敏的小鼠在口服每种蛋白质后在跑步机上跑步。在穷尽性跑步后，所有致敏小鼠的肠道糜烂程度均高于未致敏小鼠。此外，在麦胶蛋白组和谷蛋白组中，每一种小麦分离蛋白的更多口服摄入也会引起肠道糜烂。此外，我们观察到麦胶蛋白和谷蛋白从肠道渗漏到肝脏。这些结果提示小麦依赖性运动致过敏反应的主要因素可能是小麦蛋白中的麦胶蛋白和谷蛋白。麦胶蛋白是小麦依赖性运动致过敏反应的主要过敏原，它不溶于盐溶液，而食物过敏原通常是可溶的。结果表明，不溶性麦胶蛋白在胆汁乳化条件下被蛋白酶消化，吸收平稳，并保持抗原活性。对未致敏小鼠口服各分离蛋白后，检测各蛋白的消化率和吸收率。小鼠口服麦胶蛋白可明显观察到胃肠道粘膜病变，H-E染色可使小鼠小肠绒毛明显脱落。由此提示，麦胶蛋白消化产生的某种肽与乳糜泻的致病肽相同，对小肠黏膜具有毒性作用。此外，演习使损害更加严重。少

项目成果

Wheat-dependent exercise-induced anaphylaxis in mice is caused by gliadin and glutenin treatments

小麦依赖性运动引起的小鼠过敏反应是由麦醇溶蛋白和麦谷蛋白治疗引起的

• 发表时间: 2005
• 期刊: Immunol Lett. 102
• 作者: Kozai H;Yano H;Matsuda T;Kate Y.
• 通讯作者: Kate Y.

Wheat-dependent exercise-induced anaphylaxis in nice is caused by gliadin and glutenin treatments.

尼斯中依赖小麦的运动引起的过敏反应是由麦醇溶蛋白和麦谷蛋白治疗引起的。

• 发表时间: 2005
• 期刊: Immunol Lett. 102
• 作者: Kozai H;Yano H;Matsuda T;Kato Y
• 通讯作者: Kato Y

The "hygiene hypothesis" and exercise : Does exercise prevent allergic disorders?

“卫生假说”和运动：运动可以预防过敏性疾病吗？

• 发表时间: 2005
• 期刊: Kawasaki Medical Welfare Journal 15(1)
• 作者: Yano H.;Shiva D.;Kozai H.;Kato Y.
• 通讯作者: Kato Y.

Exercise training suppresses expression of tumor necrosis factor-a mRNA by adipocytes in noninsulin-dependent diabetes mellitus mice

运动训练抑制非胰岛素依赖型糖尿病小鼠脂肪细胞肿瘤坏死因子-a mRNA 的表达

• 发表时间: 2004
• 期刊: Kawasaki J Med Welfare 10
• 作者: H.Amaoka;H.Matsuzaki;H.Yano;S.Onodera
• 通讯作者: S.Onodera

Meat-killed propionibacterium acnes up-regulates toll-like receptor 4 on macrophages.

肉灭活的痤疮丙酸杆菌上调巨噬细胞上的 Toll 样受体 4。

• 发表时间: 2005
• 期刊: Kawasaki J.Medical Welfare 11・1
• 作者: Shiva D.;Matsumoto T.;Yano H.
• 通讯作者: Yano H.