Analysis of the amino-carbonyl complexes in commercial milk products affected milk allergy

研究人员对名古屋市和其他地区的1514名幼儿园儿童进行了食物过敏调查。患病比例为10%，1岁前发病占40.6%。鸡蛋和奶制品是他们的主要过敏原。患者儿童的断奶饮食和乳汁的种类没有差异。在有过敏儿童的家庭中，更多的家庭成员易患过敏。患有过敏症的孩子的母亲在怀孕和哺乳期间非常喜欢食物。在本研究中，我们使用该单克隆抗体特异性检测脱脂牛奶、婴儿奶粉、巴氏杀菌牛奶和乳饮料等商业乳制品中作为潜在牛奶过敏原的乳糖蛋白美拉德加合物。在所有8种婴儿奶粉样品中检测到高灵敏度的美拉德加合物，但在原料奶中检测不到。此外，蛋白质印迹分析表明，该单克隆抗体对高分子量乳蛋白（乳铁蛋白、BSA、IgG）和酪蛋白均有较强的染色。在6份脱脂牛奶样品中也检测了乳糖蛋白美拉德加合物。在脱脂牛奶中，单克隆抗体对低分子乳蛋白（如β - lg和α - la）以及酪蛋白具有较高的反应性。在巴氏杀菌牛奶的情况下，美拉德加合物几乎没有检测到在LLT牛奶。因此，该单克隆抗体可用于乳糖蛋白氨基羰基加合物的特异性检测以及食品和食品复杂混合物体系中乳糖修饰乳蛋白的分析。

The food allergies were investigated in 1,514 children at nursery schools in Nagoya city and others. The percentage of diseases was 10%, and 40.6% of patients got sick before the age of one. Egg and milk products were the main allergens for them. The kinds of weaning diets and lactation milks taken were not different among the patient children. In the families with allergic children more members of the families were predispositioned to suffer allergies. Mothers whose children suffered allergies were very fond of foods in their pregnancy and lactation periods.In the present study, we used the monoclonal antibody for specific detection of lactose-protein Maillard adducts as potential milk allergens in commercial milk products such as skim milks, infant powdered milks, pasteurized milks and milk beverages. Meilard adducts were detected with high sensitivity in all eight infant milk samples tested but not in raw milk by ELISA. Furthermore, protein blotting analysis demonstrated that high molecular weight milk proteins such as lactoferrin, BSA and IgG as well as caseins in the infant milks were stained strongly with the monoclonal antibosy. Lactose-protein Maillard adducts were also detected immunochemically in 6 skim milk samples. In the case of skim milks, the high reactivity of monoclonal antibody was observed in lower molecular milk proteins such as beta-LG and alpha-LA as well as caseins. In the case of pasteurized milks, Maillard adducts were hardly detected in LLT milks.Thus this monoclonal antibody is useful for the specific detection of lactose-protein amino carbonyl adducts and the analysis of lactose-modified milk proteins in the complex mixture systems of foods and food products.