Suppression of exercise-induced anaphylaxis by intake allergen with some food components

通过摄入某些食物成分的过敏原来抑制运动引起的过敏反应

• 批准号: 14580152
• 负责人: KATO Yasuko
• 金额: $ 2.62万
• 依托单位: KAWASAKI UNIVERSITY OF MEDICAL WELFARE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14580152/
• 关键词: exercise induced anaphylaxis; locomotion; voluntary physical activity; pectin; lactic acid drink; rectal temperature; B10.A mice; anaphylaxis score; 卵白リゾチーム; B10Aマウス; アナフィラキシー抑制; 回転ケージ; 急性運動 感作マウス; 小腸粘膜組織; 肝臓の蛍光発色; 特異IgE

Ovalbumin(OVA) sensitized BALB/c mice or lysozymes(Ly) sensitized B10.A mice were used to investigate the mechanisms of food dependent exercise-induced anaphylaxis. Anaphylaxis symptoms of OVA sensitized mice were clearly observed after OVA injection orally and acute exercise responsibility than that of mice treadmill exercised without OVA administration. Allergen was leaked through gastrointestinal damages and reached to a liver of Ly-sensitized mice after Ly injection orally and acute exercise responsibility than unsensitized mice. Anaphylaxis is required to evaluate the systemic symptoms, and is scored by observation of some typical symptoms such as scratching, rubbing and cyanosis. However, the scoring depends on subjective observation, resulting in the poorly quantitative evaluation for anaphylaxis rarely. Locomotion and voluntary physical activity of sensitized mice were examined for 15hr. Voluntary physical activity of sensitized mice was significantly decreased after Ly injection orally and acute exercise responsibility as same as locomotion decrease. The correlation between the symptoms scoring of anaphylaxis and rectal temperature or voluntary physical activities of the mouse was recognized in sensitized mouse model.Long time injection of pectin or a lactic acid drink to sensitized mice was suppressed their voluntary physical activity decrease. These results suggested that pectin or a lactic acid drink have protective effect for sensitized mice symptoms of anaphylaxis.

用卵清蛋白（OVA）致敏BALB/c小鼠和溶菌酶（Ly）致敏B10.A小鼠，研究食物依赖性运动过敏反应的发生机制。口服OVA致敏小鼠的过敏症状和急性运动反应性明显高于不注射OVA的小鼠。经口注射赖氨酸致敏小鼠和急性运动反应后，过敏原通过胃肠道损伤渗漏并到达肝脏，比未致敏小鼠更明显。过敏反应是评价全身症状所必需的，通过观察一些典型的症状如抓挠、摩擦和发绀来评分。然而，评分依赖于主观观察，导致很少对过敏反应进行定量评价。观察致敏小鼠15小时的自主运动和自主体力活动。口服赖氨酸后致敏小鼠的自主活动能力显著降低，急性运动反应性与运动能力下降一致。在致敏小鼠模型中，过敏反应症状评分与小鼠直肠温度和自主活动量相关，长期注射果胶或乳酸饮料可抑制致敏小鼠自主活动量的减少。提示果胶或乳酸饮料对致敏小鼠过敏症状有保护作用。

项目成果

Tsukasa Matsuda, Yasuko Kato: "Egg allergens and heat treatment hypoallergenic responses"Food Style(in Japanese). 6(1). 50-53 (2002)

Tsukasa Matsuda、Yasuko Kato：“鸡蛋过敏原和热处理低过敏反应”食品风格（日语）。

Hiromi Yano, Yasuko Kato, Tsukasa Matsuda: "Acute exercise induces gastrointestinal leakage of allergen in lysozyme-sensitized mice"Euro J of Apply Physiology. 87. 358-364 (2002)

Hiromi Yano、Yasuko Kato、Tsukasa Matsuda：“急性运动会导致溶菌酶致敏小鼠的过敏原胃肠道渗漏”，《应用生理学》欧洲杂志。

Yasuko Kato: "Allergenic activities in cooked eggs and processed foods supplemented egg"J Cookery Science of Japan(in Japanese). 35(1). 84-90 (2002)

加藤康子：“煮熟的鸡蛋和添加鸡蛋的加工食品中的过敏活性”J Cookery Science of Japan（日文）。

Keiko Ozawa, Yasuko Kato: "Allergenic activities of the various cooked eggs and processed foods supplemented with egg estimated by the amount of salt soluble ovomucoid"Nippon Shokuhinn Kagaku Kaishi(in Japanese). 49(3). 145-154 (2002)

Keiko Ozawa、Yasuko Kato：“通过盐溶性卵类粘蛋白的量估算各种煮熟的鸡蛋和添加鸡蛋的加工食品的致敏活性”Nippon Shokuhinn Kagaku Kaishi（日文）。

Yano H., Kato Y., Matuda T.: "Acute exencise induces gastro intestinal leakage of allergen in lysozyme-sensitized mice"Euro J. of Apply Physiology. 87. 358-364 (2002)

Yano H.、Kato Y.、Matuda T.：“急性运动会导致溶菌酶致敏小鼠的过敏原胃肠道渗漏”，Euro J. of Applied Physiology。