Detection of Remaining Antigenic Activeity of Major Antigen in the Process Foods

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Chicken egg and milk which are major allergenic foods for children, are used in various cooked and processed foods. But there have been few reports on their antigenic activities in the foods supplemented with either egg or milk.The antigenic activities of major egg white and milk proteins were examined in the process of pasta-and bread-making with supplementing egg white or skim milk. Particularly, ovomucoid (OM) antigenicity was examined in the process of breadmaking using competitive inhibition ELISA with rabbit anti-OM serum. OM antigenicity was completely disappeared in the baked bread but not in the kneaded and fermentated dough samples. Antigenic activity of beta-lactoglobulin (beta-LG) antigenicity remaining in bread samples containing skim milk was analyzed during several stages of bread making by the competitive ELISA using rabbit ant-beta-LG.beta-LG antigenicity was also disappered in the baked bread but not in the dough samples. OM or beta-LG in the baked bread samples was solubilized with SDS/2-ME-containing solution but not with the solution containig SDS alone, suggesting that baking with wheat flour induced the irreversible denaturation of OM or beta-LG with a disulfide exchange reaction leading to the loss of their antigenic activity which is based on the nine or two S-S bonds in a OM or beta-LG molecule. The pasta containing egg white was prepared with the process of kneading for 10 to 50 min, benching for 1 hr and boiling for 15 min. OM was disappered both in the boiled pasta which was kneaded 30 min or more and in the boiled salt solution but not in the solution boiled with the pasta which was kneaded 20 min or less.