The study of modulating effects on X-ray induced cell mutability by chaperons and SUMO.

伴侣和 SUMO 对 X 射线诱导的细胞突变性调节作用的研究。

基本信息

批准号：
16510032
负责人：
SUZUKI Nobuo
金额：
$ 2.37万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

We previously reported that modulation of cell mutability by human serum factors is associated with increased levels of protease activity. The present work is designed to examine the relationships between cell mutability and chaperons such as GRP78,GRP94,HSP27 and SUMO-3. : (1)GRP94 ; One of the chaperons, GRP94, was identified as a candidate gene for the mediator molecule by analysis of X-ray-induced protease. GRP94 was suggested to be involved in cellular X-ray resistance by the colony forming assay using siRNA for GRP94 mRNA.(2)We also identified other chaperons, HSP27, by analysis of UV-induced protease. The activity of removing 6-4 photo products after UV irradiation was decreased when expression of HSP27 was suppressed. We further identified that GRP78 is also involved in the repair mechanism for the UV-induced damage. Thus, the modulation of cell mutability by chaperons seems to be mediated though protease signaling.(3)One of ubiquitin-like proteins, SUMO-3, was identified as a candidate gene for abnormal DNA synthesis activity after X-ray irradiation. It is known that the function of SUMO-3 is regulated by specific proteases. The activity of DNA synthesis after X-ray irradiation was increased when expression of SUMO-3 was decreased. We expressed GFP-SUMO-3 fusion protein and observed a marked concentration of green fluorescence in the nuclear after X-ray irradiation.Application of a new method to evaluate oxidative stress in human serum. A test to monitor the oxidative stress by evaluating serum hydroperoxide was carried out. Serum hydrpperoxide of some patients and heavy-smokers showed higher levels than healthy volunteers.

我们先前报道说，人血清因子对细胞突变性的调节与蛋白酶活性水平的增加有关。目前的工作旨在检查细胞突变性与伴侣（例如GRP78，GRP94，HSP27和SUMO-3）之间的关系。：（1）GRP94;通过分析X射线诱导的蛋白酶，其中一种伴侣GRP94被鉴定为介体分子的候选基因。建议通过使用siRNA用于GRP94 mRNA的菌落形成测定的GRP94参与细胞X射线电阻。（2）我们还通过分析UV诱导的蛋白酶鉴定了其他伴侣HSP27。当抑制Hsp27的表达时，紫外线照射后去除6-4个照片产物的活性被降低。我们进一步确定GRP78还参与了紫外线诱导的损伤的修复机制。因此，通过蛋白酶信号传导，伴侣对细胞突变性的调节似乎是介导的。（3）X射线辐照后，泛素样蛋白SUMO-3的一种SUMO-3 SUMO-3被鉴定为异常DNA合成活性的候选基因。众所周知，SUMO-3的功能受特定蛋白酶的调节。 SUMO-3的表达降低时，X射线照射后DNA合成的活性增加。我们表达了GFP-SUMO-3融合蛋白，并观察到X射线照射后核中明显浓度的绿色荧光浓度。对一种评估人血清中氧化应激的新方法的应用。通过评估血清氢过氧化物来监测氧化应激的测试。一些患者和重烟的血清水蛋白水平比健康的志愿者高。

项目成果

期刊论文数量（33）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Enhanced expression of transferring receptor confers UV-resistance in human and monkey cells.

转移受体表达的增强赋予人和猴细胞抗紫外线能力。

DOI：
发表时间：
2005
期刊：
J. Radiat.Res. 46
影响因子：
0
作者：
Chen;Z.;Nomura;J.;Suzuki;T.;Suzuki;N.
通讯作者：
N.

Decreased cell survival and DNA repair capacity after UVC irradiation in association with down-regulation of QRP78/BiP in human RSa cells.

UVC 照射后细胞存活率和 DNA 修复能力下降与人 RSa 细胞中 QRP78/BiP 的下调相关。

DOI：
发表时间：
2005
期刊：
Exp.Cell Res. 305
影响因子：
0
作者：
Zhai;L.;Kita;K.;Wano;C.;Wu;Y.;Sugaya;S.;Suzuki;N.
通讯作者：
N.

Down-regulation of SMT3A gene expression in association with DNA synthesis induction after X-ray irradiation in nevoid basal cell carcinoma syndrome (NBCCS) cells.

痣基底细胞癌综合征 (NBCCS) 细胞 X 射线照射后 SMT3A 基因表达下调与 DNA 合成诱导相关。

DOI：
发表时间：
2005
期刊：
Mutat. Res. 578
影响因子：
0
作者：
丸山禮子;王冰;山内正剛;Miyabara Y;Nishimura N;Miyabara Y;Nishimura N;Nishimura N;Nishimura N;Sugaya S. et al.
通讯作者：
Sugaya S. et al.

Induction of uPA release in human peripheral blood lymphocytes by [deamino-Cysl,D-Arg8]-vasopressin (dDAVP).

DOI：
10.1152/ajpendo.00027.2003
发表时间：
2004-06
期刊：
American journal of physiology. Endocrinology and metabolism
影响因子：
0
作者：
Y. Yamaguchi;Kenichi Yamada;Toshikazu Suzuki;Yu‐ping Wu;K. Kita;Shunji Takahashi;M. Ichinose;N. Suzuki
通讯作者：
Y. Yamaguchi;Kenichi Yamada;Toshikazu Suzuki;Yu‐ping Wu;K. Kita;Shunji Takahashi;M. Ichinose;N. Suzuki

Leupeptin-sensitive proteases involved in cell survival early after X-ray irradiation in human RSa cells.

亮肽素敏感蛋白酶参与人 RSa 细胞 X 射线照射后早期的细胞存活。

DOI：
发表时间：
2005
期刊：
Cell Biol.Int. 29
影响因子：
0
作者：
Zhang Hong-Chang;Karata K.;Matsushita K.;Takahashi S.;Tong Xiao-Bo;Lu Jun;Zhu Chang-Lin;Sugaya S.;Suzuki T.;Suzuki N.
通讯作者：
Suzuki N.