Purification of protease induced by ultraviolet ray in human cells.
Purification of protease induced by ultraviolet ray in human cells.
批准号:
07680571
负责人:
SUZUKI Nobuo
金额:
$1.41万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1996
中文摘要
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英文摘要
Search for protease which supervises ultraviolet ray (UV)-response mechanisms in human cells was made. First, partial purification of protease itself was done by establishment of affinity chromatography. Second, several genes, which may be related with protease and/or its metabolism, were detected by differential display methods in cell lines with and without protease inducibility after UV.Thus, we could find out the methods for obtaining protease or its-related genes, although it is required for us to make more efforts.
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Suzuki,N.et al: "Correlated susceptibility between interferon-β and VV in human cell lines,F-IF^r and RSa" Int,J.Oncol.9. 297-303 (1996)
Suzuki,N.et al:“人类细胞系中干扰素-β 和 VV、F-IF^r 和 RSa 之间的相关易感性”Int,J.Oncol.9 (1996)。
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Suzuki,N.et al: "K-ras codon 12 mutation induction by VV but not by 4NQD,analyzed by PCR following differential dot-blot hybridization,in hypermutable human RSa cells" Environ.Mutagen.Res.18. 29-34 (1996)
Suzuki,N.等人:“在超可变人 RSa 细胞中,通过差异点印迹杂交后的 PCR 分析,通过 VV 而不是通过 4NQD 诱导 K-ras 密码子 12 突变”Environ.Mutagen.Res.18。
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Suzuki,N.et al: "Dipyridamole combined with tumor necrosis factor-α enhances inhibition of proliferation in human tumor cell lines" Jpn.J.Cancer Res.86. 761-769 (1995)
Suzuki, N. 等人:“双嘧达莫与肿瘤坏死因子-α 结合可增强对人肿瘤细胞系增殖的抑制”Jpn.J.Cancer Res.86 (1995)。
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Ishizuka, T., Ahmad, I., Kita, K., Sonoda, T., Ishijima, S., Sawa, K., Suzuki, N.and Tatibana, M.: "The human phosphoribosylpyrophosphate synthetase-associated protein 39 gene (PRPSAP1) is located in the chromosome region 17q24-q25." Genomics. 33. 332-334
Ishizuka, T.、Ahmad, I.、Kita, K.、Sonoda, T.、Ishijima, S.、Sawa, K.、Suzuki, N. 和 Tatibana, M.:“人类磷酸核糖焦磷酸合成酶相关蛋白 39 基因
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Suzuki, N.: "Suppression of UV- and interferon-alpha-refractoriness by antipain in human IFr cells established from RSa cells sensitive to both stimuli." J.Cell.Physiol.167. 47-51 (1996)
Suzuki, N.:“通过对两种刺激敏感的 RSa 细胞建立的人 IFr 细胞中的抗痛来抑制紫外线和干扰素 α 的不应性。”
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共 23 条
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依托单位:
海外基金