Purification of protease induced by ultraviolet ray in human cells.

人体细胞中紫外线诱导的蛋白酶的纯化。

• 批准号: 07680571
• 负责人: SUZUKI Nobuo
• 金额: $ 1.41万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680571/
• 关键词: Protease; Ultraviolet ray; Human cell; ポロテアーゼ

Search for protease which supervises ultraviolet ray (UV)-response mechanisms in human cells was made. First, partial purification of protease itself was done by establishment of affinity chromatography. Second, several genes, which may be related with protease and/or its metabolism, were detected by differential display methods in cell lines with and without protease inducibility after UV.Thus, we could find out the methods for obtaining protease or its-related genes, although it is required for us to make more efforts.

搜索监督人类细胞中紫外线（UV）反应机制的蛋白酶。首先，蛋白酶本身的部分纯化是通过建立亲和力色谱法完成的。其次，几种可能与蛋白酶和/或其代谢有关的基因是通过紫外线后有或没有蛋白酶诱导性的细胞系中的差分显示方法检测到的。

项目成果

Suzuki,N.et al: "K-ras codon 12 mutation induction by VV but not by 4NQD,analyzed by PCR following differential dot-blot hybridization,in hypermutable human RSa cells" Environ.Mutagen.Res.18. 29-34 (1996)

Suzuki,N.等人：“在超可变人 RSa 细胞中，通过差异点印迹杂交后的 PCR 分析，通过 VV 而不是通过 4NQD 诱导 K-ras 密码子 12 突变”Environ.Mutagen.Res.18。

Suzuki,N.et al: "Correlated susceptibility between interferon-β and VV in human cell lines,F-IF^r and RSa" Int,J.Oncol.9. 297-303 (1996)

Suzuki,N.et al：“人类细胞系中干扰素-β 和 VV、F-IF^r 和 RSa 之间的相关易感性”Int，J.Oncol.9 (1996)。

Suzuki,N.et al: "Dipyridamole combined with tumor necrosis factor-α enhances inhibition of proliferation in human tumor cell lines" Jpn.J.Cancer Res.86. 761-769 (1995)

Suzuki, N. 等人：“双嘧达莫与肿瘤坏死因子-α 结合可增强对人肿瘤细胞系增殖的抑制”Jpn.J.Cancer Res.86 (1995)。

Ishizuka, T., Ahmad, I., Kita, K., Sonoda, T., Ishijima, S., Sawa, K., Suzuki, N.and Tatibana, M.: "The human phosphoribosylpyrophosphate synthetase-associated protein 39 gene (PRPSAP1) is located in the chromosome region 17q24-q25." Genomics. 33. 332-334

Ishizuka, T.、Ahmad, I.、Kita, K.、Sonoda, T.、Ishijima, S.、Sawa, K.、Suzuki, N. 和 Tatibana, M.：“人类磷酸核糖焦磷酸合成酶相关蛋白 39 基因

Suzuki,N.et al: "Covrelated susceptibility between interferon-β and UV in human cell lines,F-IF^r and RSa" Int,J.Oncol.9. 297-303 (1996)

Suzuki,N.et al：“人细胞系中干扰素-β 和 UV 之间的 Covlated 易感性，F-IF^r 和 RSa”Int，J.Oncol.9 (1996)。