Purification of protease induced by ultraviolet ray in human cells.

人体细胞中紫外线诱导的蛋白酶的纯化。

• 批准号: 07680571
• 负责人: SUZUKI Nobuo
• 金额: $ 1.41万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680571/
• 关键词: Protease; Ultraviolet ray; Human cell; ポロテアーゼ

Search for protease which supervises ultraviolet ray (UV)-response mechanisms in human cells was made. First, partial purification of protease itself was done by establishment of affinity chromatography. Second, several genes, which may be related with protease and/or its metabolism, were detected by differential display methods in cell lines with and without protease inducibility after UV.Thus, we could find out the methods for obtaining protease or its-related genes, although it is required for us to make more efforts.

寻找蛋白酶，监督紫外线（UV）的反应机制，在人类细胞。首先，通过建立亲和层析对蛋白酶本身进行部分纯化。第二，利用差异显示技术，在紫外线诱导的细胞系和未诱导的细胞系中，检测到了几个可能与蛋白酶及其代谢相关的基因，从而找到了获得蛋白酶及其相关基因的方法，尽管这还需要我们做更多的努力。

项目成果

Suzuki,N.et al: "Correlated susceptibility between interferon-β and VV in human cell lines,F-IF^r and RSa" Int,J.Oncol.9. 297-303 (1996)

Suzuki,N.et al：“人类细胞系中干扰素-β 和 VV、F-IF^r 和 RSa 之间的相关易感性”Int，J.Oncol.9 (1996)。

Suzuki,N.et al: "K-ras codon 12 mutation induction by VV but not by 4NQD,analyzed by PCR following differential dot-blot hybridization,in hypermutable human RSa cells" Environ.Mutagen.Res.18. 29-34 (1996)

Suzuki,N.等人：“在超可变人 RSa 细胞中，通过差异点印迹杂交后的 PCR 分析，通过 VV 而不是通过 4NQD 诱导 K-ras 密码子 12 突变”Environ.Mutagen.Res.18。

Suzuki,N.et al: "Dipyridamole combined with tumor necrosis factor-α enhances inhibition of proliferation in human tumor cell lines" Jpn.J.Cancer Res.86. 761-769 (1995)

Suzuki, N. 等人：“双嘧达莫与肿瘤坏死因子-α 结合可增强对人肿瘤细胞系增殖的抑制”Jpn.J.Cancer Res.86 (1995)。

Ishizuka, T., Ahmad, I., Kita, K., Sonoda, T., Ishijima, S., Sawa, K., Suzuki, N.and Tatibana, M.: "The human phosphoribosylpyrophosphate synthetase-associated protein 39 gene (PRPSAP1) is located in the chromosome region 17q24-q25." Genomics. 33. 332-334

Ishizuka, T.、Ahmad, I.、Kita, K.、Sonoda, T.、Ishijima, S.、Sawa, K.、Suzuki, N. 和 Tatibana, M.：“人类磷酸核糖焦磷酸合成酶相关蛋白 39 基因

Suzuki, N.: "Suppression of UV- and interferon-alpha-refractoriness by antipain in human IFr cells established from RSa cells sensitive to both stimuli." J.Cell.Physiol.167. 47-51 (1996)

Suzuki, N.：“通过对两种刺激敏感的 RSa 细胞建立的人 IFr 细胞中的抗痛来抑制紫外线和干扰素 α 的不应性。”