Purification of protease induced by ultraviolet ray in human cells.

人体细胞中紫外线诱导的蛋白酶的纯化。

• 批准号: 07680571
• 负责人: SUZUKI Nobuo
• 金额: $ 1.41万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680571/
• 关键词: Protease; Ultraviolet ray; Human cell; ポロテアーゼ

Search for protease which supervises ultraviolet ray (UV)-response mechanisms in human cells was made. First, partial purification of protease itself was done by establishment of affinity chromatography. Second, several genes, which may be related with protease and/or its metabolism, were detected by differential display methods in cell lines with and without protease inducibility after UV.Thus, we could find out the methods for obtaining protease or its-related genes, although it is required for us to make more efforts.

人们寻找监督人体细胞中紫外线 (UV) 响应机制的蛋白酶。首先，通过建立亲和层析来部分纯化蛋白酶本身。其次，通过差异显示方法，在紫外后具有和不具有蛋白酶诱导能力的细胞系中检测到一些可能与蛋白酶和/或其代谢相关的基因。因此，我们可以找到获得蛋白酶或其相关基因的方法，尽管这还需要我们付出更多的努力。

项目成果

Suzuki,N.et al: "Correlated susceptibility between interferon-β and VV in human cell lines,F-IF^r and RSa" Int,J.Oncol.9. 297-303 (1996)

Suzuki,N.et al：“人类细胞系中干扰素-β 和 VV、F-IF^r 和 RSa 之间的相关易感性”Int，J.Oncol.9 (1996)。

Suzuki,N.et al: "K-ras codon 12 mutation induction by VV but not by 4NQD,analyzed by PCR following differential dot-blot hybridization,in hypermutable human RSa cells" Environ.Mutagen.Res.18. 29-34 (1996)

Suzuki,N.等人：“在超可变人 RSa 细胞中，通过差异点印迹杂交后的 PCR 分析，通过 VV 而不是通过 4NQD 诱导 K-ras 密码子 12 突变”Environ.Mutagen.Res.18。

Suzuki,N.et al: "Dipyridamole combined with tumor necrosis factor-α enhances inhibition of proliferation in human tumor cell lines" Jpn.J.Cancer Res.86. 761-769 (1995)

Suzuki, N. 等人：“双嘧达莫与肿瘤坏死因子-α 结合可增强对人肿瘤细胞系增殖的抑制”Jpn.J.Cancer Res.86 (1995)。

Ishizuka, T., Ahmad, I., Kita, K., Sonoda, T., Ishijima, S., Sawa, K., Suzuki, N.and Tatibana, M.: "The human phosphoribosylpyrophosphate synthetase-associated protein 39 gene (PRPSAP1) is located in the chromosome region 17q24-q25." Genomics. 33. 332-334

Ishizuka, T.、Ahmad, I.、Kita, K.、Sonoda, T.、Ishijima, S.、Sawa, K.、Suzuki, N. 和 Tatibana, M.：“人类磷酸核糖焦磷酸合成酶相关蛋白 39 基因

Suzuki, N.: "Suppression of UV- and interferon-alpha-refractoriness by antipain in human IFr cells established from RSa cells sensitive to both stimuli." J.Cell.Physiol.167. 47-51 (1996)

Suzuki, N.：“通过对两种刺激敏感的 RSa 细胞建立的人 IFr 细胞中的抗痛来抑制紫外线和干扰素 α 的不应性。”