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Dynamics of dissolved organic nitrogen in the ocean obtained by using high-sensitive and high-resolution method

高灵敏高分辨率方法获取海洋溶解有机氮动态

基本信息

批准号：
16540445
负责人：
OGAWA Hiroshi
金额：
$ 2.24万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16540445/
关键词：
Dissolved organic nitrogen(DON)Dissolved organic carbon(DOC)C : N ratio High-sensitive and high-resolution analysis Nitrogen cycling Ocean Elemental analysis C:N比

项目摘要

A high-sensitive and high-resolution method for measuring dissolved organic nitrogen (DON) in seawater has been established, which consists of a high-temperature combustion unit and a chemiluminescence detector. The international program for instrument comparison for measurement of DON in seawater confirmed that this system has a high performance to obtain accurate and precious data of DON in seawater. By using this system, the detailed distribution of DON in a variety of sea area and biological processes of DON in incubation experiments were obtained as follows: surface oceans in the western North Pacific along 165 E longitude and along A line of Oyashio region, Arakawa estuary in Tokyo Bay, and microbial decomposition process of DON in surface waters in the western. North Pacific and release process of DON by coral. The system is designed for simultaneous measurement of dissolved organic carbon (DOC) with DON, so that it has been made possible that DON dynamics is directly compared with those of DOC, and characteristics of C:N ratio of dissolved organic matter (DOM) is discussed. Consequently it was found that relatively C-rich DOM was stably accumulating in oligotrophic area and unproductive season, whereas N-rich DOM was temporally accumulating in high latitude area especially in productive season. Amino acids in DOM (Total Dissolved Amino acids: TDAA), as typical nitrogenous organic compounds and most major identifiable component of DON, were also determined for some oceanic samples. The proportions of amino acids' carbon (TDAA-C) to DOC (0.8-1.8%) and amino acids' nitrogen (TDAA-N) to DON (8-18%) were obtained. Both proportions of TDAA-C/DOC and TDAA-N/DON showed a tendency to be high in productive area of high latitude and low in oligotrophic area of low latitude, suggesting DOM in productive area contained more biogenic and fresh components, whereas more altered components mainly composed DOM in oligotrophic area.

建立了一种高灵敏度、高分辨率测定海水中溶解有机氮（DON）的方法，该方法由高温燃烧装置和化学发光检测器组成。国际海水中DON测量仪器比对计划证实，该系统具有高性能，可获得准确、珍贵的海水中DON数据。利用该系统，获得了多种海域DON的详细分布以及孵化实验中DON的生物过程如下：北太平洋西部沿165 E经度和沿着东京湾荒川河口的Oyashio地区A线的表层海洋，以及西部表层海水中DON的微生物分解过程。沿着东京湾荒川河口的Oyashio地区。西部表层海水中DON的微生物分解过程。沃茨。北太平洋及珊瑚释放DON的过程。该系统可同时测定溶解有机碳（DOC）和溶解有机氮（DON），从而实现了DON动态与DOC动态的直接对比，并探讨了溶解有机物（DOM）的碳氮比特征。结果表明，相对富C的DOM在贫营养区和非生产季节稳定积累，而富N的DOM在高纬度地区，特别是在生产季节有时间积累。作为典型的含氮有机物和DON的主要可识别组分，对部分海洋样品中的氨基酸进行了测定。氨基酸碳（TDAA-C）占DOC（0.8-1.8%）的比例和氨基酸氮（TDAA-N）占DON（8-18%）的比例。TDAA-C/DOC和TDAA-N/DON的比值均表现出高纬度生产区较高，低纬度贫营养区较低的趋势，说明生产区DOM中含有较多的生物源和新鲜组分，而贫营养区则以DOM为主。