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Effects of phytosterol content in vegetable oil on life span of salt-loaded stroke-prone spontaneously hypertensive rats (SHRSP)

植物油中植物甾醇含量对高盐脑卒中易发性高血压大鼠（SHRSP）寿命的影响

基本信息

批准号：
16590185
负责人：
OGAWA Hiroshi
金额：
$ 1.73万
依托单位：
Kinki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Recent studies using salt-loaded SHRSP reported that salt-loaded SHRSP exhibited different lifespan depending on the type of vegetable oil in their diet. In the present study, to evaluate whether the difference of phytosterol content in vegetable oil influences stroke onset and lifespan of salt-loaded SHRSP. Male SHRSP/Kpo were used at the age of 6 weeks. Test diets were prepared by using four kinds of vegetable oils which contain different amount of phytosterol. Three percent NaCl by weight was added to the test diets for acceleration of hypertension. Rats had free access to the diet and drinking water. Blood pressure was measured by the tail-pulse pickup method. The onset of stroke was judged by sudden losses of both bodyweight and food intake and characteristic symptoms.There were no significant differences in growth rate, food intake among the four experimental groups before the onset of stroke. Systolic blood pressure of the ricebran oil group tended to be higher compared with the … More other three groups. Phytosterol content in the vegetable oil shortens survival time of salt-loaded SHRSP in a dose-dependent manner, regardless of fatty acids composition of the vegetable oil.Next, to elucidate the mechanism of survival time-shortening effect of ricebran oil, we focused on the effect of ricebran oil on stroke onset and lipid metabolism in salt-loaded SHRSP. Rats were fed the test diets containing ricebran oil or soybean oil (control) as fat source and 3% NaCl for 38 days.In the serum, cholesterol and phospholipids levels were significantly higher compared with the control, which was due to the increase in the HDL fraction. It will be very important to elucidate why the HDL fraction increases and what relationship exists between stroke onset and the elevation of the HDL fraction.In the liver, cholesterol and triglyceride contents were significantly lower compared with the control. A comparative study on mRNA expression indicated that ricebran oil may inhibit HMG-CoA reductase, resulting in the reduction of hepatic cholesterol content. In addition, activation of PPAR (peroxisome proliferators-activeted receptor)α target genes may induce the reduction of hepatic triglyceride content. Conversely, reduction of LXR (liver X-receptor)α mRNA expression may induce the reduction of ABC (ATP-binding cassette transporter) G5 and ABCG8 mRNA expressions, which indicates accumulation of phytosterol in the liver. This hepatic accumulation of phytosterol might be closely related to the survival time-shortening effect of ricebran oil. Less

最近的研究使用盐加载SHRSP报道，盐加载SHRSP表现出不同的寿命取决于他们的饮食中的植物油的类型。本研究旨在探讨植物油中植物甾醇含量的差异是否影响盐负荷SHRSP的脑卒中发病和寿命。雄性SHRSP/Kpo在6周龄时使用。试验用四种植物油配制了不同植物甾醇含量的试验日粮。在试验饮食中加入重量百分比为3%的NaCl，以加速高血压。大鼠可自由获取饮食和饮用水。采用尾脉拾波法测量血压。以体重和摄食量的突然下降及特征性症状判断卒中的发生，发病前4个实验组的生长速度、摄食量无显著性差异。米糠油组的收缩压有升高的趋势， ...更多信息其他三组。植物油中的植物甾醇含量以剂量依赖的方式缩短了盐负荷SHRSP的生存时间，而与植物油的脂肪酸组成无关。接下来，为了阐明米糠油缩短生存时间的作用机制，我们重点研究米糠油对盐负荷SHRSP中风发作和脂质代谢的影响。以米糠油或大豆油（对照）为脂肪源的试验饲料和3%NaCl喂养大鼠38天，血清中胆固醇和磷脂水平显著高于对照组，这是由于HDL组分的增加。这将是非常重要的，以阐明为什么高密度脂蛋白组分增加，以及什么关系之间存在的中风发作和高密度脂蛋白组分的升高。在肝脏中，胆固醇和甘油三酯含量显着低于对照组。对mRNA表达的比较研究表明，米糠油可能抑制HMG-CoA还原酶，导致肝脏胆固醇含量降低。此外，过氧化物酶体增殖物激活受体（peroxisome proliferators-activeted receptor，PPAR）α靶基因的激活可能导致肝脏甘油三酯含量的降低。相反，LXR（肝脏X受体）α mRNA表达的减少可能会导致ABC（ATP结合盒转运蛋白）G5和ABCG 8 mRNA表达的减少，这表明植物甾醇在肝脏中的积累。这种植物甾醇在肝脏中的积累可能与米糠油缩短存活时间的作用密切相关。少

项目成果

期刊论文数量（37）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

易发生中风的 SHR、Wistar-Kyoto 大鼠和 Wistar 大鼠肠道脂质代谢与菌株相关的差异。

DOI：
发表时间：
2006
期刊：
Clin.Exp.Hypertens. 28(5)(In press)
影响因子：
0
作者：
Hibino;H.;Fujita;A.;Iwai;K.;Yamada;M.;Kurachi;Y.;M.Yamada et al.;山田充彦;Yamamoto K.;Ogawa H.;Yamamoto K.;Ogawa H.
通讯作者：
Ogawa H.

Effects of aleurone flour on blood pressure and lipid metabolism in SHRSP fed a cholesterol diet.

糊粉粉对饲喂胆固醇饮食的 SHRSP 的血压和脂质代谢的影响。

DOI：
发表时间：
2004
期刊：
Bimigizyutsukennkyuukaishi. 5
影响因子：
0
作者：
Kimura;M.;Hisaharu Kohzuki;Ogata A;Hamada T. et al.;Ogawa H.;Hamada T. et al.;Kohzuki H;Ogawa H.;Inukai Y;Kato M.et al.;西山哲成;Kohzuki H;小川博;Kato M.et al.;西村直記;OgawaH.;Hamada T. et al.;藤野英己;緒方昭広;Fujino H;Terasaka S. et al.;Yamamoto K.;Sato M;Rho J.-y.et al.;Kamisaka T.;緒方昭広;Kohzuki H;Terasaka S. et al.;菅屋潤壹;Ogawa H.
通讯作者：
Ogawa H.

Hypotensive and lipid regulatory actions of 4-hydroxyderricin, a chalcone from Angelica keiskei, in stroke-prone spontaneously hypertensive

4-羟基德里辛（明日叶中的一种查耳酮）对易发生中风的自发性高血压的降血压和脂质调节作用

DOI：
发表时间：
2005
期刊：
Clin.Exp.Pharmacol.Physiol. 32(12)
影响因子：
0
作者：
Kimura;M.;Hisaharu Kohzuki;Ogata A;Hamada T. et al.;Ogawa H.
通讯作者：
Ogawa H.

Improve Effect of Peruvian herbs on Lipid Metabolism of Diet-induced Hyperlipidemic Stroke-Prone Spontaneously Hypertensive Rats(SHRSP)

秘鲁草药对饮食诱导的高脂血症易发生中风的自发性高血压大鼠（SHRSP）脂质代谢的改善作用

DOI：
发表时间：
2004
期刊：
Clin.Exp.Hypertens. 26(5)
影响因子：
0
作者：
Kimura;M.;Hisaharu Kohzuki;Ogata A;Hamada T. et al.;Ogawa H.;Hamada T. et al.;Kohzuki H;Ogawa H.;Inukai Y;Kato M.et al.;西山哲成;Kohzuki H;小川博;Kato M.et al.;西村直記;OgawaH.;Hamada T. et al.;藤野英己;緒方昭広;Fujino H;Terasaka S. et al.;Yamamoto K.
通讯作者：
Yamamoto K.

Effects of vegetable oils containing different amount of phytosterol on blood pressure, stroke onset and life span in salt-loaded stroke-prone spontaneously hypertensive rats (SHRSP).

含有不同量植物甾醇的植物油对高盐脑卒中易发性高血压大鼠（SHRSP）血压、脑卒中发病和寿命的影响。

DOI：
发表时间：
2006
期刊：
Clin.Exp.Hypertens. 28(5)(In press)
影响因子：
0
作者：
Yoko Inukai;Junichi Sugenoya;Masko Kato;Naoki Nishimura;Tetsunari Nishiyama;Takaaki Matsumoto;Miki Sato;Akihiro Ogata;Yumiko Taniguchi;Atsushi Osada;Ogawa H.;渡辺和人;Yamamoto K.
通讯作者：
Yamamoto K.