Target identification and physiological analyses of bovine prolactin-related proteins.

牛催乳素相关蛋白的靶标鉴定和生理分析。

• 批准号: 16580235
• 负责人: TAKAHASHI Toru
• 金额: $ 2.37万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580235/
• 关键词: bovine; placenta; prolactin; matrix; alkaline phosphatase; fusion protein; collagen; type IV; プロラクチン関連タンパク質; Nb2; 7Sドメイン; NC1ドメイン

The bovine placenta produces an array of proteins structurally similar to pituitary prolactin (PRL). At least ten genes of the bovine placental PRL family, including bovine placental lactogen (bPL) and ten bovine PRL-related proteins bPRP-I to-XI), encode hormones/cytokines predicted to be involved in the establishment and maintenance of pregnancy. Targets and biological roles for most members of the bovine PRL family have yet to be specified. This study focused on three members of bovine PRL family, bPL, bPRP-I and bPRP-VI. An alkaline phosphatase (AP) tagging strategy was used to monitor interactions of the ligands with their targets. AP-bPRP-I and APbPRP-VI specifically bound to tissue sections of the bovine placentome. AP-bPRP-I and AP-bPRP-VI binding within the placentome mimicked the distribution of the extracellular matrix (ECM). Consequently, AP fusion protein binding to individual extracellular matrix components (heparin, laminin, fibronectin, type I collagen and type IV collagen) was evaluated. AP-bPRP-I specifically bound to type IV collagen, but not to the other ECM components. AP-bPRP-VI exhibited weak interactions with ECM components, while AP-bPL and AP did not show significant binding to any of the ECM components. Binding of AP-bPRP-I to type IV collagen was concentration dependent, influenced by salt concentrations, and specific to the N-terminal cross-linking domain (7S) of type IV collagen but not its triple-helical domain. The interaction of bPRP-I with type IV collagen suggests that bPRP-I accumulates in the extracellular matrix where it likely acts on cells traversing the bovine placentome.

牛胎盘产生一系列结构上类似于脑垂体催乳素(PRL)的蛋白质。至少有10个牛胎盘催乳素家族的基因，包括牛胎盘催乳素(BPL)和10个牛PRL相关蛋白(bPRP-I to-XI)，编码激素/细胞因子，被预测参与妊娠的建立和维持。牛催乳素家族大多数成员的靶点和生物学作用尚未明确。本研究以牛催乳素家族的三个成员bpl、bPRP-I和bPRP-VI为研究对象。碱性磷酸酶(AP)标记策略被用来监测配体与其靶标的相互作用。AP-bPRP-I和APbPRP-VI可与牛胎盘组织切片特异性结合。AP-bPRP-I和AP-bPRP-VI在胎盘内的结合模拟了细胞外基质(ECM)的分布。因此，AP融合蛋白与单个细胞外基质成分(肝素、层粘连蛋白、纤维连接蛋白、I型胶原和IV型胶原)结合进行了评估。AP-bPRP-I与IV型胶原特异性结合，但不与其他细胞外基质成分结合。AP-bPRP-VI与ECM组分相互作用较弱，而AP-BPL和AP与ECM组分无明显结合。AP-bPRP-I与IV型胶原的结合是浓度依赖的，受盐浓度的影响，并且特异性地结合到IV型胶原的N端交联域(7S)，而不是它的三螺旋结构域。BPRP-I与IV型胶原的相互作用表明，bPRP-I积聚在细胞外基质中，在那里它可能作用于穿越牛胎盘的细胞。

项目成果

Generation and purification of recombinant bovine pregnancy associated glycoprotein.

重组牛妊娠相关糖蛋白的产生和纯化。

• 发表时间: 2004
• 期刊: Vet J. 168 (3)
• 作者: Ushizawa K;Kaneyama K;Takahashi T;Tokunaga T;Tsunoda Y;Hashizume K;Nakano et al.;Ushizawa et al.;Takahashi et al.;Ushizawa et al.;Ushizawa et al.;Patel at al.
• 通讯作者: Patel at al.

Tokunaga T, Tsunoda Y, Tsujimoto G, Hashizume K. cDNA microarray analysis of bovine embryo gene expression profiles during the pre-implantation period.

Tokunaga T、Tsunoda Y、Tsujimoto G、Hashizume K. 植入前牛胚胎基因表达谱的 cDNA 微阵列分析。

• 发表时间: 2004
• 期刊: Reprod Biol Endocrinol. 2
• 作者: Ushizawa K;Herath CB;Kaneyama K;Shiojima S;Hirasawa A;Takahashi T;Imai K;Ochiai K
• 通讯作者: Ochiai K

Cloning and expression of a new member of prolactin-related protein in bovine placenta : bovine prolactin-related protein-VII.

牛催乳素相关蛋白新成员：牛催乳素相关蛋白-VII的克隆及表达。

• 发表时间: 2005
• 期刊: Biochem Biophys Res Commun. 326(2)
• 作者: Ushizawa K;Kaneyama K;Takahashi T;Tokunaga T;Tsunoda Y;Hashizume K
• 通讯作者: Hashizume K

Gene expression profiles of novel caprine placental prolactin-related proteins similar to bovine placental prolactin-related proteins

• DOI: 10.1186/1471-213x-7-16
• 发表时间: 2007-03-15
• 期刊: BMC DEVELOPMENTAL BIOLOGY
• 作者: Ushizawa, Koichi;Takahashi, Toru;Hashizume, Kazuyoshi
• 通讯作者: Hashizume, Kazuyoshi

Develop-mental aberrations of liver gene expression in bovine fetuses derived from somatic cell nuclear transplantation

• DOI: 10.1089/clo.2006.8.79
• 发表时间: 2006-06-01
• 期刊: CLONING AND STEM CELLS
• 作者: Herath, Chandana B.;Ishiwata, Hiroko;Hashizume, Kazuyoshi
• 通讯作者: Hashizume, Kazuyoshi