Biological activities of ovine and bovine placental prolactin family proteins

绵羊和牛胎盘催乳素家族蛋白的生物活性

• 批准号: 22580327
• 负责人: TAKAHASHI Toru
• 金额: $ 2.91万
• 依托单位: National Institute of Agrobiological Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580327/
• 关键词: ウシ; 胎盤; プロラクチン; カテプシン; 血管内皮細胞; MMP; 7S; IV型コラーゲン

Bovine prolactin-related protein-I (PRP1) is a non-classical member of prolactin (PRL) family proteins. However, its function is still unknown. PRL, when cleaved by cathepsin D and matrix metalloproteinases (MMPs), resulted in N-terminal 16 kDa fragments (16K-PRL) that have antiangiogenetic properties in human and rodents. We examined the possibility of similar activity of bovine PRP1. PRP1 (normally 33 kDa) was cleaved by cathepsins (CTSs), MMPs, and bovine cotyledonary-conditioned medium (BCCM), and generated mainly 26 kDa N-terminal fragments. Two specific enzyme families, CTSs and MMPs cleaved intact PRP1, and BCCM also contained PRP1 cleavage activity. Bioactivity of cleaved PRP1 was examined in a cell proliferation assay using bovine brain vascular endothelial cells. The cleaved PRP1 stimulated the proliferation of endothelial cells in vitro. The endothelial cell proliferation activity of cleaved PRP1 may be shared in specific bovine placentomal angiogenesis.

牛催乳素相关蛋白i （PRP1）是催乳素（PRL）家族蛋白的非经典成员。然而，它的功能仍然未知。PRL被组织蛋白酶D和基质金属蛋白酶（MMPs）切割后，在人和啮齿类动物中产生n端16kda片段（16K-PRL），具有抗血管生成特性。我们检测了牛PRP1类似活性的可能性。PRP1（通常为33 kDa）被组织蛋白酶（CTSs）、MMPs和牛子叶条件培养基（BCCM）切割，主要产生26 kDa的n端片段。两个特定的酶家族，CTSs和MMPs可以切割完整的PRP1， BCCM也具有PRP1的切割活性。利用牛脑血管内皮细胞进行细胞增殖实验，检测了裂解后的PRP1的生物活性。裂解后的PRP1在体外刺激内皮细胞的增殖。裂解的PRP1的内皮细胞增殖活性可能在特定的牛胎盘血管生成中共享。

项目成果

着床とプロラクチン-ウシPRL/GHファミリーは着床誘導物質か?-

着床和催乳素 - 牛 PRL/GH 家族是着床诱导剂吗？

• 发表时间: 2011
• 期刊: Hormone Frontier in Gynecolog
• 作者: 橋爪一善;高橋透;木崎景一
• 通讯作者: 木崎景一

Cleaved bovine prolactin-related protein-I stimulates vascular endothelial cell proliferation

• DOI: 10.1016/j.mce.2010.03.008
• 发表时间: 2010-07-29
• 期刊: MOLECULAR AND CELLULAR ENDOCRINOLOGY
• 影响因子: 4.1
• 作者: Ushizawa, Koichi;Takahashi, Toru;Hashizume, Kazuyoshi
• 通讯作者: Hashizume, Kazuyoshi

Expression of extracellular matrix metalloproteinase inducer (EMMPRIN) and its related extracellular matrix degrading enzymes in the endometrium during estrous cycle and early gestation in cattle

• DOI: 10.1186/1477-7827-8-60
• 发表时间: 2010-06-11
• 期刊: REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
• 影响因子: 4.4
• 作者: Mishra, Birendra;Kizaki, Keiichiro;Hashizume, Kazuyoshi
• 通讯作者: Hashizume, Kazuyoshi

A novel factor for the regulation of utero-placental angiogenesis

调节子宫胎盘血管生成的新因子

• 发表时间: 2010
• 作者: Takahashi T;Ushizawa K;Hosoe M;Kizaki K;Hashizume K
• 通讯作者: Hashizume K

着床とプロラクチンーウシPRL/GHファミリーは着床誘導物質か?

着床和催乳素 - 牛 PRL/GH 家族是着床诱导剂吗？

• 发表时间: 2011
• 期刊: HORMONE FRONTIER IN GYNECOLOGY
• 作者: 橋爪一善;高橋透;木崎景一郎
• 通讯作者: 木崎景一郎