Delivery of genomic protein drug using the chemical modification with basic peptide and biodegradable minicapsules

使用碱性肽和可生物降解微胶囊进行化学修饰来递送基因组蛋白药物

• 批准号: 16590128
• 负责人: YAMAMOTO Akira
• 金额: $ 2.3万
• 依托单位: Kyoto Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590128/
• 关键词: Basic peptide; Cell penetrating peptide; Chitosan capsules; Biodegradable; Protein drug; Genomic drug; Transmucosal absorption; Absorption enhancement

The objective of this study was to estimate the colon-specific delivery of [Asu^<1,7>]-eel calcitonin (ECT) using chitosan capsules in rats. The intestinal absorption of ECT was evaluated by measuring the plasma calcium levels after oral administration of the chitosan capsules containing ECT and different combinations of additives. A marked decrease in plasma calcium levels was observed following the oral administration of chitosan capsules containing ECT, S-nitroso-N-acetyl-DL-penicillamine (SNAP), sodium glycocholate, bacitracin and aprotinin, as compared with capsules containing only ECT or capsules containing ECT with SNAP only. The hypocalcemic effect started 6-8 hr after oral administration of capsules and sustained for 24 hr. These findings suggest that colon-specific delivery of ECT can be achieved using chitosan capsules and these additives may be useful for improving the colonic absorption of ECT in rats.

本研究的目的是评估壳聚糖胶囊在大鼠结肠特异性递送[Asu^<1,7>]-鳗鱼降钙素（ECT）的效果。口服含有ECT的壳聚糖胶囊及不同添加剂组合后，通过测定血浆钙水平来评价ECT的肠道吸收。口服含有ECT、s -亚硝基-n -乙酰基- dl -青霉胺（SNAP）、糖胆酸钠、杆菌肽和抑酶蛋白的壳聚糖胶囊后，血浆钙水平明显下降，与仅含ECT胶囊或仅含SNAP的ECT胶囊相比。低钙效果在口服胶囊后6-8小时开始，持续24小时。这些发现表明，壳聚糖胶囊可以实现ECT的结肠特异性递送，这些添加剂可能有助于改善大鼠结肠对ECT的吸收。

项目成果

Colon-specific delivery and enhanced colonic absorption of (Asu~<1,7>)-eel calcitonin using chitosan capsules containing various additives in rats.

使用含有各种添加剂的壳聚糖胶囊在大鼠体内对(Asu~<1,7>)-鳗鱼降钙素进行结肠特异性递送并增强结肠吸收。

• 发表时间: 2006
• 期刊: Int.J.Pharm. (in press)
• 作者: Gihan Fetih;Fawsia Habib;Naoki Okada;Takuya Fujita;Mohammed Attia;Akira Yamamoto
• 通讯作者: Akira Yamamoto

Colon-specific delivery and enhanced colonic absorption of (Asu^<1,7>)-eel calcitonin using chitosan capsules containing various additives in rats.

使用含有各种添加剂的壳聚糖胶囊在大鼠体内进行结肠特异性递送并增强(Asu^<1,7>)-鳗鱼降钙素的结肠吸收。

• 发表时间: 2006
• 期刊: Int.J.Pharm. (in press)
• 作者: Gihan Fetih;Fawsia Habib;Naoki Okada;Takuya Fujita;Mohammed Attia;Akira Yamamoto
• 通讯作者: Akira Yamamoto

Improvement of absorption enhancing effects of n-dodecyl-β-D-maltopyranoside by its colon-specific delivery using chitosan capsules

• DOI: 10.1016/j.ijpharm.2004.12.017
• 发表时间: 2005-04-11
• 期刊: INTERNATIONAL JOURNAL OF PHARMACEUTICS
• 影响因子: 5.8
• 作者: Fetih, G;Lindberg, S;Yamamoto, A
• 通讯作者: Yamamoto, A