Delivery of genomic protein drug using the chemical modification with basic peptide and biodegradable minicapsules

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The objective of this study was to estimate the colon-specific delivery of [Asu^<1,7>]-eel calcitonin (ECT) using chitosan capsules in rats. The intestinal absorption of ECT was evaluated by measuring the plasma calcium levels after oral administration of the chitosan capsules containing ECT and different combinations of additives. A marked decrease in plasma calcium levels was observed following the oral administration of chitosan capsules containing ECT, S-nitroso-N-acetyl-DL-penicillamine (SNAP), sodium glycocholate, bacitracin and aprotinin, as compared with capsules containing only ECT or capsules containing ECT with SNAP only. The hypocalcemic effect started 6-8 hr after oral administration of capsules and sustained for 24 hr. These findings suggest that colon-specific delivery of ECT can be achieved using chitosan capsules and these additives may be useful for improving the colonic absorption of ECT in rats.