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Molecular mechanisms of postnatal differentiation of epididymis regulated by sex hormones

性激素调控附睾产后分化的分子机制

基本信息

批准号：
16590136
负责人：
KOMIYAMA Masatoshi
金额：
$ 2.05万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590136/
关键词：
Epididymis Gene expression Development Differentiation DNA microarray Mouse Aquaporin

项目摘要

Epididymis is morphologically divided into the caput, corpus and cauda regions, which have their own specific luminal environments. In this study, gene expression in the mouse epididymis was examined during postnatal development to analyze molecular mechanisms of functional differentiation of epididymal regions. DNA microarray analysis revealed that gene expression pattern remarkably changes during postnatal three weeks and reach to the adult pattern by 3 weeks of age. These gene expressions were quite different among the caput, corpus and cauda regions. Next, male newborn mice were exposed to diethylstilbestrol, a synthetic estrogen, to examine how sex hormones affect the postnatal development of epididymis. This treatment caused relative stromal overgrowth of epididymis and high expression of type I collagen genes (col1a1 and col1a2). It was revealed by in situ hybridization analysis that the relative stromal overgrowth is caused by increase in expression of col1a1 and col1a2 genes in each stromal cell. Further, a novel gene (Serpina1f) and 4 unknown mouse epididymis-specific genes (Lcp1, Cuzd1, Teddm1 and Wfdc16) were identified by DNA microarray and real-time RT-PCR analyses. The expression of these genes was confirmed to increase rapidly during juvenile period. Their expression areas and regulation of expression of these genes by sex hormones were also analyzed in this study. Gene expression of aquaporins (AQPs), water channel proteins, was also analyzed by real-time RT-PCR. It was found that 10 types of AQP (AQP1-9 and 11) express in mouse epididymis and expression of many of AQPs decrease by excess of estrogens.

附睾在形态上分为头区、体区和尾区，它们都有自己特定的管腔环境。在本研究中，检查了小鼠出生后发育过程中附睾中的基因表达，以分析附睾区域功能分化的分子机制。 DNA微阵列分析显示，基因表达模式在出生后三周内发生显着变化，并在三周龄时达到成人模式。这些基因表达在头、体和尾部区域之间有很大差异。接下来，雄性新生小鼠接触己烯雌酚（一种合成雌激素），以检查性激素如何影响产后附睾的发育。这种治疗导致附睾间质相对过度生长和 I 型胶原蛋白基因（col1a1 和 col1a2）的高表达。原位杂交分析表明，相对基质过度生长是由每个基质细胞中 col1a1 和 col1a2 基因表达增加引起的。此外，通过 DNA 微阵列和实时 RT-PCR 分析鉴定了一个新基因 (Serpina1f) 和 4 个未知的小鼠附睾特异性基因 (Lcp1、Cuzd1、Teddm1 和 Wfdc16)。这些基因的表达被证实在青少年时期迅速增加。本研究还分析了它们的表达区域以及性激素对这些基因表达的调节。还通过实时 RT-PCR 分析了水通道蛋白 (AQP)（水通道蛋白）的基因表达。研究发现，小鼠附睾中有 10 种 AQP（AQP1-9 和 11）表达，并且许多 AQP 的表达会因雌激素过量而减少。

项目成果

期刊论文数量（22）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Association of increased type I collagen expression and relative stromal overgrowth in mouse epididymis neonatally exposed to diethylstilbestrol

DOI：
10.1002/mrd.20347
发表时间：
2005-11
期刊：
Molecular Reproduction and Development
影响因子：
2.5
作者：
K. Yamazaki;H. Fukata;T. Adachi;H. Tainaka;Masao Kohda;M. Yamazaki;K. Kojima;K. Chiba;C. Mori;M. Komiyama
通讯作者：
K. Yamazaki;H. Fukata;T. Adachi;H. Tainaka;Masao Kohda;M. Yamazaki;K. Kojima;K. Chiba;C. Mori;M. Komiyama

Toxicogenomic analysis of human umbilical cords to establish a new risk assessment of human fetal exposure to multiple chemicals. In: Handbook of Toxicogenomics

对人类脐带进行毒理学分析，以建立人类胎儿接触多种化学物质的新风险评估。