Cancer diagnosis with Photo dynamic Raman spectroscopy

利用光动态拉曼光谱诊断癌症

• 批准号: 16591307
• 负责人: KONNO Hiroyuki
• 金额: $ 2.3万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591307/
• 关键词: Raman; gastric cancer; photodynamic diagnosis; 内視鏡的光学癌診断; 主成分分析; 近赤外励起ラマン分光; 分光学的診断

Photodynamic diagnosis (PDD) with Raman spectroscopy is expecting to be a novel modality for cancer diagnosis. Since 2003, we developed Raman Diagnostic System (RDS) for gastrointestinal malignancy. The goal of RDS is real-time optical diagnosis during endoscopy or surgery. Endoscopic RDS enables to be a real-time implementation to assess the mucosal pathology in gastrointestinal tract. Intraoperative RDS has the possibility of real-time diagnosis for lymph node metastasis or peritoneal dissemination, resulting that the patients with gastrointestinal cancer can receive less-invasive and most suitable surgery. Our RDS has a novel detector with high sensitivity in which the influence of autofluorescence on Raman signal intensity could be reduced in a limited range at 1064 nm excitation. Previously, we reported the preliminary study of RDS, in which Raman spectra were assessed with principle component analysis (PCA). The sufficient accuracy was obtained. Next we improved RDS to increase the clinical usefulness. We examined gastric cancer specimens removed by endoscopic biopsy. The results had better reproducibility and reliability without PCA. In the cancer diagnosis, the sensitivity was 72 %, specificity was 70 % and accuracy was 71 %. And we have confirmed stability of measurements and the level of accuracy. We are improving RDS to elevate the accuracy.We are going to exam the gastric cancer specimens removed by operation. We can measure the spectrum of cancer lesion to normal tissue continuously.RDS is a novel diagnostic implementation for the gastrointestinal cancer. The present data strongly suggest that improvement of both instruments and methods of RDS increases the accuracy of cancer diagnosis and the application of RDS to endoscopy or surgery is expected to achieve real-time cancer detection without histological examination.

拉曼光谱光动力诊断（PDD）有望成为癌症诊断的一种新方式。自2003年以来，我们开发了胃肠道恶性肿瘤拉曼诊断系统（RDS）。 RDS 的目标是在内窥镜检查或手术期间进行实时光学诊断。内窥镜 RDS 能够实时评估胃肠道粘膜病理。术中RDS有可能实时诊断淋巴结转移或腹膜播散，使胃肠癌患者能够接受微创、最适合的手术。我们的RDS拥有一种新型高灵敏度检测器，在1064 nm激发下可以在有限范围内减少自发荧光对拉曼信号强度的影响。之前，我们报道了RDS的初步研究，其中拉曼光谱通过主成分分析（PCA）进行评估。获得了足够的精度。接下来我们改进了 RDS 以提高临床实用性。我们检查了通过内窥镜活检取出的胃癌标本。无需PCA，结果具有更好的重现性和可靠性。在癌症诊断中，敏感性为72%，特异性为70%，准确性为71%。我们已经确认了测量的稳定性和准确性水平。我们正在改进RDS，以提高准确性。我们将对手术取出的胃癌标本进行检查。我们可以连续测量癌症病变对正常组织的光谱。RDS是一种新颖的胃肠道癌症诊断方法。目前的数据强烈表明，RDS仪器和方法的改进提高了癌症诊断的准确性，并且RDS在内窥镜或手术中的应用有望实现无需组织学检查的实时癌症检测。