Research of the sentinel lymph node biopsy in the gastrointestinal cancer, breast cancer and thyroid cancer : Analysis using real-time RT-PCR

胃肠癌、乳腺癌和甲状腺癌前哨淋巴结活检研究：实时 RT-PCR 分析

• 批准号: 16591370
• 负责人: NAKANO Shogo
• 金额: $ 2.37万
• 依托单位: Aichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591370/
• 关键词: follicular adenoma; follicular carcinoma; CEA mRNA; RT-PCR; 乳癌; 甲状腺癌; 消化器癌; センチネルリンパ節

BACKGROUND : We prospectively tested whether circulating tumor cells could be detected in peripheral blood of patients with thyroid tumors by a reverse transcription-polymerase chain reaction (RT-PCR) to detect carcinoembryonic antigen (CEA) messenger RNA (mRNA). METHODS : We assayed for CEA mRNA by RT-PCR in peripheral blood sampled before and 2 to 3 weeks after curative surgery for thyroid tumors in 121 patients. Blood samples from 7 patients with chronic thyroiditis and 7 healthy subjects served as controls. RESULTS : No control samples were positive for CEA mRNA by RT-PCR. Of 121 preoperative samples from patients with thyroid tumor, 6 were positive (5.0%). Preoperative frequencies of CEA mRNA positivity in benign tumor, papillary carcinoma, follicular variant papillary carcinoma, minimally invasive follicular carcinoma, and widely invasive follicular carcinoma were 0%, 0%, 0%, 44.4% (4/9), and 50.0% (2/4), respectively. Among positive patients only one, who had widely invasive follicular carcinoma, remained positive after surgery. CONCLUSIONS : RT-PCR detection of tumor cells in preoperative blood often can distinguish malignant from benign follicular thyroid tumors.

背景技术背景：我们前瞻性地测试了是否可以通过逆转录-聚合酶链反应（RT-PCR）检测癌胚抗原（CEA）信使RNA（mRNA）在甲状腺肿瘤患者的外周血中检测到循环肿瘤细胞。方法：采用RT-PCR方法检测121例甲状腺肿瘤患者术前及术后2 ~ 3周外周血CEA mRNA的表达。7例慢性甲状腺炎患者和7例健康人的血液样本作为对照。结果：RT-PCR检测未检测到CEA mRNA阳性。在121例甲状腺肿瘤患者术前标本中，6例阳性（5.0%）。良性肿瘤、乳头状癌、滤泡型乳头状癌、微侵袭性滤泡状癌、广泛侵袭性滤泡状癌术前CEA mRNA阳性率分别为0%、0%、0%、44.4%（4/9）、50.0%（2/4）。在阳性患者中，只有一个，广泛浸润性滤泡癌，手术后仍呈阳性。结论：术前血液中肿瘤细胞的RT-PCR检测通常可以区分甲状腺滤泡性肿瘤的良恶性。

项目成果

一般病棟における緩和ケアマニュアル(ビスフォスフォネート使用の実際)

普通病房姑息治疗手册（双膦酸盐的实际使用）

• 发表时间: 2005
• 作者: 中野正吾;小川道雄
• 通讯作者: 小川道雄

進行癌(Stage III,IVa)の治療方針 乳癌

晚期癌症（III、IVa期）乳腺癌的治疗政策

• 发表时间: 2005
• 期刊: 外科治療 92巻
• 作者: 原尾美智子;城塚透子;中野正吾;毛利有佳子;秋月美和;佐藤孝至;山下純一
• 通讯作者: 山下純一

一般病棟における緩和ケアマニュアル

普通病房姑息治疗手册

• 发表时间: 2005
• 作者: 中野正吾;小川道雄
• 通讯作者: 小川道雄

Neutrophil elastase and breast cancer

中性粒细胞弹性蛋白酶与乳腺癌

• 发表时间: 2005
• 期刊: Breast Cancer (in press)
• 作者: Yamashita J;et al.
• 通讯作者: et al.

Comparison of video-assisted minithoracotomy and standard open thoracotomy for the treatment of non-small cell lung cancer

电视辅助小切口与标准开胸手术治疗非小细胞肺癌的比较

• 期刊: Minimal Invas Surg Appl Technol (in press)
• 作者: Tashima T;et al.
• 通讯作者: et al.