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The studies of the functional significance of regenerating gene (Reg) in failing hearts.

再生基因（Reg）在衰竭心脏中的功能意义的研究。

基本信息

批准号：
16591405
负责人：
TANIGUCHI Shigeki
金额：
$ 1.86万
依托单位：
NARA MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

1.To demonstrate the activation profiles of Reg gene expression in the rat heart, we generated two models of acute heart failure - myocardial infarction by coronary artery ligation and pressure-overload by aortic constriction. The expression levels of Reg mRNA and Reg-receptor mRNA in the heart tissue were quantified by a real-time RT-PCR. There was a positive correlation between the level of Reg mRNA expression and the severity of acute heart stress. Reg-1 mRNA activation was observed mainly in the atria, and Reg-receptor mRNA was expressed intensely in damaged ventricles. In conclusion, our results demonstrate for the first time the presence of the Reg/Reg-receptor system in damaged hearts.2.We found REG-1 protein expression in the human hearts obtained from autopsied patients who died of myocardial infarction. REG protein was immunohistochemically stained in a fine granular pattern in the cytoplasm of cardiomyocytes. Tissue components other than cardiomyocytes appeared not to be involved in the secretion of REG protein.3.Cultured cardiomyocytes (H9c2) were stretched 10% or 15% on flexible silicon rubber chamber for 1, 3, and 6 hours, using stretching apparatus (ST-140, Strex Inc). No Reg gene expression was detected in the stretched cardiomyocytes. Because the expression profiles of ANP and BNP mRNA observed in the present study were different from previously reported literatures, further studies were needed to elucidate the Reg gene expression in the stretched cardiomyocytes.

1.为了证明大鼠心脏中Reg基因表达的激活情况，我们生成了两种急性心力衰竭模型——冠状动脉结扎引起的心肌梗死和主动脉缩窄造成的压力超负荷。通过实时 RT-PCR 定量心脏组织中 Reg mRNA 和 Reg 受体 mRNA 的表达水平。 Reg mRNA表达水平与急性心脏应激严重程度呈正相关。 Reg-1 mRNA 激活主要在心房中观察到，Reg 受体 mRNA 在受损的心室中强烈表达。总之，我们的研究结果首次证明受损心脏中存在Reg/Reg受体系统。2.我们在死于心肌梗塞的尸检患者心脏中发现REG-1蛋白表达。 REG 蛋白在心肌细胞的细胞质中被免疫组织化学染色为细颗粒状。除心肌细胞外的组织成分似乎不参与REG蛋白的分泌。3.使用拉伸装置(ST-140，Strex Inc)将培养的心肌细胞(H9c2)在柔性硅橡胶室上拉伸10%或15%1、3和6小时。在拉伸的心肌细胞中没有检测到Reg基因表达。由于本研究中观察到的ANP和BNP mRNA的表达谱与之前报道的文献不同，因此需要进一步的研究来阐明拉伸心肌细胞中Reg基因的表达。