The studies of the functional significance of regenerating gene (Reg) in failing hearts.

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1.To demonstrate the activation profiles of Reg gene expression in the rat heart, we generated two models of acute heart failure - myocardial infarction by coronary artery ligation and pressure-overload by aortic constriction. The expression levels of Reg mRNA and Reg-receptor mRNA in the heart tissue were quantified by a real-time RT-PCR. There was a positive correlation between the level of Reg mRNA expression and the severity of acute heart stress. Reg-1 mRNA activation was observed mainly in the atria, and Reg-receptor mRNA was expressed intensely in damaged ventricles. In conclusion, our results demonstrate for the first time the presence of the Reg/Reg-receptor system in damaged hearts.2.We found REG-1 protein expression in the human hearts obtained from autopsied patients who died of myocardial infarction. REG protein was immunohistochemically stained in a fine granular pattern in the cytoplasm of cardiomyocytes. Tissue components other than cardiomyocytes appeared not to be involved in the secretion of REG protein.3.Cultured cardiomyocytes (H9c2) were stretched 10% or 15% on flexible silicon rubber chamber for 1, 3, and 6 hours, using stretching apparatus (ST-140, Strex Inc). No Reg gene expression was detected in the stretched cardiomyocytes. Because the expression profiles of ANP and BNP mRNA observed in the present study were different from previously reported literatures, further studies were needed to elucidate the Reg gene expression in the stretched cardiomyocytes.