Xenotransplantation of ex vivo gene trandected cardiomyocytes

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We examined xenotransplantion of fetal murine cardiomyocytes to rats, which was made myocardial infarction with coronary artery ligated. In the infracted area, transplanted fetal cardiomyocytes was necrosis due to ischemia, but are alive without hyper-acute rejection in the border zone of normal and infracted area. In addition a little immuno-suppressive agent (cyclosporine 5mg/kg) made donor cells alive over thirty days. We think xenotransplantation of fetal cardiomyocytes are useful for therapy of heart failure.The gene tranduction technologies have greatly expand the potential feasibility of immunotheapy against various disorders including malignancy. A critical impediment of non-viral vector system is the relatively poor Transduction/expression efficiency, which interrupts their real applications to clinical usage. However, the Epstein-Barr virus(EBV)-based episomal plasmid vectors in combination with various delivery vehicles bring very-strong expression in variety of human cells. We tried gene transduction of interferon-β gene to tumor cells with this vector system, and examined anti-tumor effect of gene manipulation. Remarkable suppression of tumor cells growth was obtained by transfection with the EBV-based vector, while transfer of same gene by a conventional Plasmid vector resulted in moderate suppressive effect. Non-viral means equipped with EBV-based plasmid vector offer novel strategies of genetic manipulation applicable to immuno-gene therapy.