Construction of 3D cardiac tissue: alignment and maturation of cells, and improvement of contraction force by extensional stimulation
Construction of 3D cardiac tissue: alignment and maturation of cells, and improvement of contraction force by extensional stimulation
批准号:
22591552
负责人:
TANIGUCHI Shigeki
金额:
$2.5万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
2010
资助国家:
日本
项目状态:
已结题
起止时间:
2010 至 2012
中文摘要
对构建人工心脏组织的培养条件进行了研究。与市售胶原蛋白相比,国产胶原蛋白增加了心肌细胞在组织中的比例,增加了与钙处理相关的基因的表达,并增加了收缩蛋白的比例。在胶原水凝胶中的三维培养本身也可以诱导ES细胞向心肌细胞分化。
英文摘要
Culture condition for constructing artificial cardiac tissue was examined. Housemade collagen increased the ratio of cardiac cells in the tissue, the expression of genes relating calcium handling, and the ratio of protein for contraction compared with commercial available collagen. It was also revealed that three-dimensional culture in collagen hydrogel itself might induce differentiation of ES cells into cardiac cells.
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依托单位:
海外基金