Construction of 3D cardiac tissue: alignment and maturation of cells, and improvement of contraction force by extensional stimulation

3D 心脏组织的构建：细胞的排列和成熟，以及通过拉伸刺激改善收缩力

• 批准号: 22591552
• 负责人: TANIGUCHI Shigeki
• 金额: $ 2.5万
• 依托单位: Nara Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22591552/
• 关键词: 再生医療; 組織工学; 細胞・組織; 心筋細胞; ES細胞; 人工心筋組織

Culture condition for constructing artificial cardiac tissue was examined. Housemade collagen increased the ratio of cardiac cells in the tissue, the expression of genes relating calcium handling, and the ratio of protein for contraction compared with commercial available collagen. It was also revealed that three-dimensional culture in collagen hydrogel itself might induce differentiation of ES cells into cardiac cells.

对构建人工心脏组织的培养条件进行了研究。与市售胶原蛋白相比，国产胶原蛋白增加了心肌细胞在组织中的比例，增加了与钙处理相关的基因的表达，并增加了收缩蛋白的比例。在胶原水凝胶中的三维培养本身也可以诱导ES细胞向心肌细胞分化。