Research for wound healing using an artificial skin model composed with cultured human skin cells.

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Artificial skin cultures made using cultured human skin cells have attracted attention as experimental models, as well as for clinical applications. However artificial skin has few chemical articles that undergo damage and observed progress. We created a number of artificial skin models. These artificial models were irradiated with a CO2 laser at various strengths. We evaluated the degree of damage and the time course of tissue regeneration in these models using pathological and immunohistochemical techniques.With mild irradiation conditions, epidermal keratinocytes in the deep layer survived. The damaged surface was then repaired by the growth of undamaged epidermal keratinocytes in a short time. In contrast, when the dermal layer was damaged, a longer time period was required for keratinocyte regeneration. From these results, we conclude that surviving epidermal keratinocytes in irradiated skin sites are important for skin tissue regeneration. When laser irradiation is mild and keratinocytes remain at the irradiated area, epidermal keratinocytes develop many layers and the repaired epidermis is thick and firm. In contrast, when the dermal cells are damaged, the repaired epidermis is weaker with fewer layers of epidermal keratinocytes.We also studied the effects of human placental extract by using an artificial skin model with laser irradiation. The samples were irradiated with a CO2 laser and cultured in a medium with 1% HPE, 0.1% HPE, 0% HPE (control). The conclusion was that HPE produced a specific density affect as an accelerator of wound healing, but successive amounts of HPE produced a specific density affect as an inhibitor.Our observations resemble physiological wound healing processes within the living body. We believe that this artificial skin model is unique and useful for experimental studies of skin regeneration after irradiation with various lasers.