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Researches for wound healing using a hybrid type artificial skin model retrofitted with human immune cells

使用人体免疫细胞改造的混合型人造皮肤模型进行伤口愈合研究

基本信息

批准号：
20592107
负责人：
KAO Bunsho
金额：
$ 2.83万
依托单位：
Showa University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2008
资助国家：
日本
起止时间：
2008 至 2010
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592107/
关键词：
移植・再生医療再生医学ヒト皮膚モデル培養皮膚

项目摘要

This study investigated the behavior of macrophages in the wound healing process in human skin using a new artificial skin model with macrophages. The experimental group (the artificial skin model with human monocyte) and control group 1 (without monocyte) were irradiated with a CO_2 laser, and these were compared with control group 2 (no laser irradiation with monocyte). Tissue samples were collected for 7 days after laser irradiation and the degree of tissue damage and the process of regeneration were evaluated using hematoxylin-eosin and immunohistochemical staining.The monocytes changed to macrophages in the samples. The number of macrophages increased and the size was larger after laser irradiation in the experimental group from the third day and reached a peak on the fifth day. The macrophages were activated both in the irradiated area and in the whole sample. MCP-1, a chemokine produced by inflammatory reactions, was identified in the epidermal layer from the keratinocytes and the dermal epidermal border region. These results suggest that MCP-1 from macrophages induced an acceleration of the re-epithelization in the epidermis, while also inducing wound healing.In conclusion, this new artificial skin model with macrophages demonstrated increased tissue interactions, especially in inflammatory reactions.

本研究采用一种新的含巨噬细胞的人工皮肤模型，研究了巨噬细胞在人皮肤创伤愈合过程中的行为。实验组（含人单核细胞的人工皮肤模型）和对照1组（不含单核细胞）用CO_2激光照射，并与对照2组（不含单核细胞）进行比较。激光照射后7 d，收集组织标本，用苏木精-伊红染色和免疫组化染色观察组织损伤程度和再生过程，标本中单核细胞向巨噬细胞转化。实验组巨噬细胞从照射后第3天开始数量增多，体积增大，第5天达到高峰。巨噬细胞在照射区域和整个样品中都被激活。MCP-1是一种由炎症反应产生的趋化因子，在角质形成细胞和真皮表皮边缘区域的表皮层中被鉴定。这些结果表明，MCP-1从巨噬细胞诱导的表皮再生的加速，同时也诱导伤口愈合。总之，这种新的人工皮肤模型与巨噬细胞表现出增加的组织相互作用，特别是在炎症反应。