Prevention of Nitric Oxide Production and Cell Deaths by Estrogen in Estrogen Receptor Expressed Rheumatoid Arthritis Fibroblasts

雌激素在雌激素受体表达的类风湿关节炎成纤维细胞中预防一氧化氮产生和细胞死亡

• 批准号: 16591891
• 负责人: SUENAGA Shigeaki
• 金额: $ 1.86万
• 依托单位: Kagoshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591891/
• 关键词: Estrogen receptor; Estrogen; Fluorescent bioimaging; Reactive oxygen species; Nitric oxide; Apoptosis; Transfection; RNAi; エスロゲン; NO

We performed a series of experiments hypothesizing that pathogenesis of RA is initially caused by generations of ROS from mitochondria and binding with NO results in lipid peroxidation and subsequent apoptosis. In addition we hypothesized that estrogen regulates mitochondrial ROS and NO productions and lipid peroxidation as a potential factors for the inhibition of apoptosis in synovial fibroblasts in RA, and these effects were dependent on the expressions of ERs.This study indicated that (1)in RA, mitochondrial ROS and NO would be key players to cause RA pathogenesis by subsequent increase in lipid peroxidation and apoptosis ; (2)estrogen and the binding to ER plays an important role in protecting cells against cytokine-induced apoptotic cell death by controlling the generations of mitochondrial ROS and NO and lipid peroxidation in synovial fibroblasts ; (3)the differential expression of ER_results in different generations of mitochondrial ROS, NO, lipid peroxidation and subsequent apoptosis. These results support the overall hypothesis that intracellular mitochondrial ROS and NO generations and production of lipid peroxidation products subsequently accelerates apoptotic cell death and they are regulated by binding of estrogen to ER_in synovial fibroblasts.

我们进行了一系列的实验，假设RA的发病机制最初是由线粒体产生的ROS引起的，并与NO结合导致脂质过氧化和随后的凋亡。本研究提示：（1）在RA中，线粒体ROS和NO可能是导致脂质过氧化和细胞凋亡的重要因素;（2）雌激素及其与雌激素受体（ER）的结合通过控制滑膜成纤维细胞线粒体ROS和NO的生成及脂质过氧化，在对抗苦参碱诱导的细胞凋亡中起重要作用：（3）ER_2的差异表达导致线粒体ROS、NO、脂质过氧化及随后的凋亡的不同生成。这些结果支持了细胞内线粒体ROS和NO的产生以及脂质过氧化产物的产生随后加速细胞凋亡的总体假设，并且它们是通过雌激素与滑膜成纤维细胞中的ER_2结合来调节的。

项目成果

Oxidative Stress, Disease and Cancer. Intracellular oxidative stress caused by ionizing radiation

氧化应激、疾病和癌症。

• 发表时间: 2006
• 作者: Majima HJ;Indo HP;Tomita K;Suenaga S;et al.
• 通讯作者: et al.

Intracellular oxdative stress caused by ionizing radiation.

电离辐射引起的细胞内氧化应激。

• 发表时间: 2006
• 期刊: Oxidative Stress, Disease and Cancer(Imperial College Press, London, UK)
• 作者: Majima HJ;Indo HP;Tomita K;Suenaga S;Motoori S;Kato H;Yen HC;Ozawa T.
• 通讯作者: Ozawa T.