Influence of Missing Molar Teeth (SDA) on Stress Hormone in Saliva

本研究旨在探讨磨牙缺失与应激激素的关系。本文研究了25例单侧或双侧臼齿缺失（缩短牙弓）患者，其中女性18例，男性7例，平均年龄62.1岁。他们被分为5组，分别由5名牙医治疗义齿。所有受试者在牙科椅上保持直立姿势，并要求咀嚼棉花卷（唾液盒）2分钟，以收集唾液样本。唾液收集的阶段分别是访谈后立即、治疗前、治疗后立即、第一次就诊时出院前、随后每次就诊时、治疗前后。唾液样品在检测前立即冷冻-50℃保存，用ELISA试剂盒检测皮质醇和嗜铬粒蛋白A。采用重复测量的方差分析方法对RPD治疗前后的比较进行分析。治疗前首次就诊时游离唾液皮质醇和嗜铬粒蛋白浓度显著高于治疗后。2 .当患者使用可摘局部义齿并对义齿满意时，唾液皮质醇和嗜铬粒蛋白A的浓度较其他访视时明显降低。患者取下RPD 3天后，唾液皮质醇和嗜铬粒蛋白A浓度显著升高。目前的数据表明，SDA是压力来源的一个因素，治疗RPD应减少压力。然而，由于这些结果可能涉及许多其他因素，因此需要一个新的实验模型来进行分析。

The purpose of this study is to elucidated relationship between missing molar teeth and the stresses hormone.Twenty-five patients (18 females and 7 males, average 62.1 year-old) whose unilateral or bilateral molar teeth were missing (Shortened Dental Arch 'SDA') were participated in this study. They were divided into 5 groups and were treated their denture by 5 dentists. All subjects were rested in an upright position on the dental chair, and asked to chew a cotton roll (salivette) for 2 min, which was to collect their saliva sample. The stages of saliva collection were immediately after interviewing, just before treatment, immediately after treatment, and before leaving hospital on the first visit, and at each subsequent visit, before and after treatment.Saliva samples were immediately frozen-50℃ for storage prior to assay, and cortisol and chromogranin A was measured by ELISA kit. Comparisons between before and after treatment of RPD were analyzed using an ANOVA with repeated measures.1. The concentrations of free salivary cortisol and chromogranin before treatment for the RPD at the first time of visit were significantly higher than those after treatment.2. When the patients were provided the removable partial denture (RPD) and satisfied with their denture, the concentration of salivary cortisol and chromogranin A decreased significantly compare with those at the other visits.3. After the patients removed their RPD for 3 days, the concentration of salivary cortisol and chromogranin A increase significantly.The present data suggest that SDA was a factor for a source of stress and that treatment of RPD should reduce the stress. However, as these results might involve many other factors, a new experimental model should be required for the analysis.