Development oh tissue-engineered bone with bio absovbency which holds occlusal support.

开发具有生物吸收性的组织工程骨，可提供咬合支撑。

• 批准号: 16591976
• 负责人: BABA Shunsuke
• 金额: $ 2.11万
• 依托单位: Foundation for Biomedical Research and Innovation
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591976/
• 关键词: Regenerative medicine; scafold

In this research, it is in the development of the scaffold to which it gives priority to a dynamic function of the bone by using the cultured cell and the biological absorption scaffold technology. The scaffold uses the growth of the osteobrast cell to treat to natural teeth and implant surroundings so that it may make amends for the cell when urge function is possessed and the reasonable amount is not obtained only by the cell. However, neither a hydroxyapatite nor a calcium phosphate system material that is the scaffold marketed now are appreciable in not the one in the point of regeneration of the bone that finishes being satisfactory but osteobrast cell formation. These problems are not to be able to promote the ability of the cell growth enough by the osteobrast cell of the self-origin and combining scaffolds. In addition, it is not a biological absorption scaffold to expect to replace native bone for a certain period in the function as the scaffold. Equip absorption, the inside p … More ore must be high, and the osteobrast cell and the blood vessel must go into it easily, and the structure with the function that the load of the occlusal force is possible in the mouth become conditions of functionally evaluating the reproductive bone. There is a feature in the point to reproduce surrounding bones of natural teeth an bones in the implant surroundings by MSCs of the self-origin that is the origin of the alveolar bone, enamel, the cement Quality, the periodontal membrane, and developing the culture bone of occlusal support that can be endured by using the scaffold with a biological absorption where the optimization design was done. In this research, by designing a biological absorption scaffold, it was assumed that the structure of the scaffold material corresponding to the revitalization degree of the culture osteobrast cell was able to be optimized, and the scaffold to be able to expect the healing of wound and the bone formation promotion action in addition to the more efficient bone reproduction was developed. The cell was settled on in the type scaffold and the plan sowing was settled on including knitting Cago referring to the result of a relation between PRP and the cell, and current clinical researches. This is the cloth structure finished knitting with one PLA fiber as a scaffold for the tissue regeneration. This scaffold has the cave internally in the Cago shape. It is because of raising the implantation rate by putting the cell in here. The one that has been put to practical use now forms the cell, and this scaffold can be cut into a necessary size by sowing internally in the reason to have the opening at the medical practice, and when operating, can be formed according to the coloboma part though is difficult sowing in the scaffold. Moreover, it is useful in the point that is the structure to which it can circulate around the body fluid by the interface in the scaffold and the coloboma part. Not only the bond efficiency of the cell to sow in the scaffold since the culture cell condition was examined to measure the optimization of the cell to sow here but also the mechanics characteristic of the scaffold having to take part became clear. In addition, there was a problem in bend strength was supplemented from the necessity for improving operativeness to surroundings of the PLA fiber with the binder of PCL. Moreover, the MSCs was bonded to squirrel-cage scaforld, and it cultured it. The biodegradation speed of the scaffold manufactured from PLA is slow as a result, externals remained being maintained for a long term of six months or more as for the scaffold though the cell reproduction and the differentiation induction to the osseous system were possible. The adjustment of the absorption degradation rate of the scaffold and the bone regeneration speed of the culture osteoblast became an important problem from these. When having experimented on the animal to confirm in Vivo of the PLA fiber, the reproduction of the bone would be confirmed in 12 weeks and the absorption of the scaffold was confirmed for that. An excellent result was obtained compared with the transplant only of the cell. Less

在本研究中，利用培养细胞和生物吸收支架技术，优先考虑骨骼的动态功能，是在支架的开发中。该支架利用成骨细胞的生长对天然牙和种植体周围环境进行治疗，在具有督促功能且仅靠细胞无法获得合理数量的情况下对细胞进行补偿。然而，无论是羟基磷灰石还是磷酸钙系统材料，目前市场上销售的支架材料，在骨再生方面都不是令人满意的，而是成骨细胞的形成。这些问题是由自身来源的成骨细胞与支架结合不能充分促进细胞生长的能力。此外，它不是一种生物吸收支架，期望在一定时期内取代天然骨作为支架的功能。装备吸收率高，内部p值高，成骨细胞和血管容易进入，口腔内可能承受咬合力载荷的结构成为评价生殖骨功能的条件。在这一点上有一个特点，即通过自身来源的间充质干细胞在种植体周围复制天然牙齿的周围骨骼，这是牙槽骨、牙釉质、水泥质量、牙周膜的来源，并通过使用具有生物吸收的支架来发展咬合支持的培养骨，该支架进行了优化设计。本研究通过设计一种生物吸收支架，设想能够优化培养成骨细胞的活化程度所对应的支架材料结构，开发出既能预期伤口愈合又能促进骨形成的支架，同时还能实现更高效的骨再生。参考PRP与细胞的关系及目前临床研究结果，将细胞固定在类型支架上，并确定包括编织Cago在内的计划播种。这是用一根聚乳酸纤维作为支架进行组织再生的织物结构成品编织。这个支架内部有卡戈形状的洞穴。这是因为把细胞放在这里可以提高植入率。目前已投入实际使用的是形成细胞，这种支架在医疗实践中可以通过在有开口的原因中播种来切割成必要的尺寸，在操作时，虽然在支架中播种困难，但可以根据结肠部分形成。此外，它的有用之处在于，它可以通过支架和结肠部分的界面在体液周围循环。不仅检测了细胞在支架中播种的结合效率，因为培养细胞的条件被检查，以衡量细胞在这里播种的优化，而且支架必须参与的力学特性变得清晰。此外，从提高PLA纤维对周围环境的操作性的需要出发，补充了用PCL粘结剂制备PLA纤维的抗弯强度存在的问题。并将MSCs与鼠笼支架结合培养。聚乳酸制备的支架生物降解速度慢，体外维持时间长达6个月或更长，但细胞繁殖和诱导分化为骨系统是可能的。调节支架的吸收降解速率和培养成骨细胞的骨再生速度成为一个重要的问题。在动物实验中确认PLA纤维在体内的存在，12周后确认骨的再生，并确认支架的吸收性。与单纯的细胞移植相比，取得了较好的效果。少

项目成果

Differential Regulation by IL-10 and EGF of Expression Three Different Hyaluronan Synthases in Oral Mucosal of Epithelial Cells and Fibroblasts and Dermal Fibroblasts : Quantitative Analysis Using Real-Time RT-PCR.

IL-10 和 EGF 对上皮细胞、成纤维细胞和真皮成纤维细胞口腔粘膜中三种不同乙酰透明质酸合成酶表达的差异调节：使用实时 RT-PCR 进行定量分析。

• 发表时间: 2004
• 期刊: J Invest Dermatol 122
• 作者: Yoichi Yamada;Naoki Itano;Ken-Ichiro Hata;Minoru Ueda;Koji Kimata
• 通讯作者: Koji Kimata

Simultaneous implant placement and bone regeneration around dental implants using tissue-engineered bone with fibrin glue, mesenchymal stem cells and platelet-rich plasma

• DOI: 10.1111/j.1600-0501.2006.01246.x
• 发表时间: 2006-10-01
• 期刊: CLINICAL ORAL IMPLANTS RESEARCH
• 影响因子: 4.3
• 作者: Ito, Kenji;Yamada, Yoichi;Ueda, Minoru
• 通讯作者: Ueda, Minoru

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal Fibroblasts : Quantitative Analysis Using Real-Time RT-PCR.

IL-1β 和 EGF 对口腔粘膜上皮细胞、成纤维细胞和真皮成纤维细胞中三种不同透明质酸合成酶表达的差异调节：使用实时 RT-PCR 进行定量分析。

• 发表时间: 2004
• 期刊: J Invest Dermatol 122
• 作者: Yoichi Yamada;Naoki Itano;Ken-Ichiro Hata;Minoru Ueda;Koji Kimata
• 通讯作者: Koji Kimata

生分解性樹脂複合材料により構成される組織再生用足場(スカフォールド)

可生物降解树脂复合材料组织再生支架

• 发表时间: 2004

Osteogenic potential of injectable tissue-engineered bone : a comparison among autogenous bone, bone substitute (Bio-Oss(^【○!R】)), platelet-rich plasma(PRP), and tissue-engineered bone with respect to their mechanical properties and histological findings

可注射组织工程骨的成骨潜力：自体骨、骨替代物（Bio-Oss（^【○！R】））、富血小板血浆（PRP）和组织工程骨的机械性能比较和组织学发现

• 发表时间: 2005
• 期刊: Journal of Biomedical Materials Research 73A
• 作者: Ito;K.;Yamada;Y.;Nagasaka;T.;Baba;S.;Ueda,M.
• 通讯作者: Ueda,M.