Development oh tissue-engineered bone with bio absovbency which holds occlusal support.

开发具有生物吸收性的组织工程骨，可提供咬合支撑。

• 批准号: 16591976
• 负责人: BABA Shunsuke
• 金额: $ 2.11万
• 依托单位: Foundation for Biomedical Research and Innovation
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591976/
• 关键词: Regenerative medicine; scafold

In this research, it is in the development of the scaffold to which it gives priority to a dynamic function of the bone by using the cultured cell and the biological absorption scaffold technology. The scaffold uses the growth of the osteobrast cell to treat to natural teeth and implant surroundings so that it may make amends for the cell when urge function is possessed and the reasonable amount is not obtained only by the cell. However, neither a hydroxyapatite nor a calcium phosphate system material that is the scaffold marketed now are appreciable in not the one in the point of regeneration of the bone that finishes being satisfactory but osteobrast cell formation. These problems are not to be able to promote the ability of the cell growth enough by the osteobrast cell of the self-origin and combining scaffolds. In addition, it is not a biological absorption scaffold to expect to replace native bone for a certain period in the function as the scaffold. Equip absorption, the inside p … More ore must be high, and the osteobrast cell and the blood vessel must go into it easily, and the structure with the function that the load of the occlusal force is possible in the mouth become conditions of functionally evaluating the reproductive bone. There is a feature in the point to reproduce surrounding bones of natural teeth an bones in the implant surroundings by MSCs of the self-origin that is the origin of the alveolar bone, enamel, the cement Quality, the periodontal membrane, and developing the culture bone of occlusal support that can be endured by using the scaffold with a biological absorption where the optimization design was done. In this research, by designing a biological absorption scaffold, it was assumed that the structure of the scaffold material corresponding to the revitalization degree of the culture osteobrast cell was able to be optimized, and the scaffold to be able to expect the healing of wound and the bone formation promotion action in addition to the more efficient bone reproduction was developed. The cell was settled on in the type scaffold and the plan sowing was settled on including knitting Cago referring to the result of a relation between PRP and the cell, and current clinical researches. This is the cloth structure finished knitting with one PLA fiber as a scaffold for the tissue regeneration. This scaffold has the cave internally in the Cago shape. It is because of raising the implantation rate by putting the cell in here. The one that has been put to practical use now forms the cell, and this scaffold can be cut into a necessary size by sowing internally in the reason to have the opening at the medical practice, and when operating, can be formed according to the coloboma part though is difficult sowing in the scaffold. Moreover, it is useful in the point that is the structure to which it can circulate around the body fluid by the interface in the scaffold and the coloboma part. Not only the bond efficiency of the cell to sow in the scaffold since the culture cell condition was examined to measure the optimization of the cell to sow here but also the mechanics characteristic of the scaffold having to take part became clear. In addition, there was a problem in bend strength was supplemented from the necessity for improving operativeness to surroundings of the PLA fiber with the binder of PCL. Moreover, the MSCs was bonded to squirrel-cage scaforld, and it cultured it. The biodegradation speed of the scaffold manufactured from PLA is slow as a result, externals remained being maintained for a long term of six months or more as for the scaffold though the cell reproduction and the differentiation induction to the osseous system were possible. The adjustment of the absorption degradation rate of the scaffold and the bone regeneration speed of the culture osteoblast became an important problem from these. When having experimented on the animal to confirm in Vivo of the PLA fiber, the reproduction of the bone would be confirmed in 12 weeks and the absorption of the scaffold was confirmed for that. An excellent result was obtained compared with the transplant only of the cell. Less

本研究主要是利用培养细胞和生物可吸收支架技术，研制出一种以动态功能性骨为主的支架。该支架利用成骨细胞的生长对天然牙和种植体周围环境进行处理，使其在具有促动功能而单靠细胞不能获得合理数量时，可以对细胞进行弥补。然而，目前市售的作为支架的羟基磷灰石和磷酸钙系统材料都不是在完成令人满意的骨再生方面，而是在成骨细胞形成方面是值得赞赏的。这些问题主要是自体成骨细胞与复合支架不能充分促进细胞生长的能力。此外，它不是一种生物吸收支架，期望在一定时期内替代天然骨发挥支架的作用。设备吸收，内吸 ...更多信息 骨密度必须高，成骨细胞和血管必须容易进入其中，并且具有咬合力负荷在口腔中可能的功能的结构成为功能评价生殖骨的条件。本研究的特点是利用自体来源的MSCs再生天然牙周围骨和种植体周围骨，即牙槽骨、牙釉质、牙骨质、牙周膜的来源，并利用优化设计的生物可吸收支架材料，开发出可耐受咬合支持的培养骨。在本研究中，通过设计生物吸收支架，假设能够优化与培养成骨细胞的再生程度相对应的支架材料的结构，并且开发了除了更有效的骨再生之外还能够期望伤口愈合和骨形成促进作用的支架。参照PRP与细胞关系的结果，结合目前的临床研究，将细胞固定在该型支架上，并将种植方案确定为包括编织Cago。这是用一根PLA纤维编织完成的布结构，作为组织再生的支架。这个脚手架内部有一个Cago形状的洞穴。这是因为通过将细胞放入这里来提高植入率。已经投入实际使用的支架现在形成细胞，并且该支架可以通过在医疗实践中在内部播种以具有开口而切割成所需的尺寸，并且当操作时，可以根据缺损部分形成，尽管在支架中播种是困难的。此外，它在以下方面是有用的，即它可以通过支架和缺损部分中的界面围绕体液循环的结构。不仅由于培养细胞条件而检查细胞在支架中播种的结合效率以测量细胞在这里播种的优化，而且必须参与的支架的力学特性也变得清楚。另外，由于需要用PCL粘合剂改善PLA纤维对周围环境的操作性，因此在补充弯曲强度方面存在问题。将骨髓间充质干细胞与鼠笼式支架复合培养，结果表明，PLA制成的支架生物降解速度慢，虽然可以进行细胞增殖和向成骨系统分化，但其表面仍能维持6个月以上。因此，如何调节支架材料的吸收降解速度和培养成骨细胞的骨再生速度成为一个重要的问题。当在动物身上进行实验以确认PLA纤维的体内时，将在12周内确认骨的再生，并确认支架的吸收。与仅移植细胞相比，获得了优异的结果。少

项目成果

Differential Regulation by IL-10 and EGF of Expression Three Different Hyaluronan Synthases in Oral Mucosal of Epithelial Cells and Fibroblasts and Dermal Fibroblasts : Quantitative Analysis Using Real-Time RT-PCR.

IL-10 和 EGF 对上皮细胞、成纤维细胞和真皮成纤维细胞口腔粘膜中三种不同乙酰透明质酸合成酶表达的差异调节：使用实时 RT-PCR 进行定量分析。

• 发表时间: 2004
• 期刊: J Invest Dermatol 122
• 作者: Yoichi Yamada;Naoki Itano;Ken-Ichiro Hata;Minoru Ueda;Koji Kimata
• 通讯作者: Koji Kimata

Simultaneous implant placement and bone regeneration around dental implants using tissue-engineered bone with fibrin glue, mesenchymal stem cells and platelet-rich plasma

• DOI: 10.1111/j.1600-0501.2006.01246.x
• 发表时间: 2006-10-01
• 期刊: CLINICAL ORAL IMPLANTS RESEARCH
• 影响因子: 4.3
• 作者: Ito, Kenji;Yamada, Yoichi;Ueda, Minoru
• 通讯作者: Ueda, Minoru

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal Fibroblasts : Quantitative Analysis Using Real-Time RT-PCR.

IL-1β 和 EGF 对口腔粘膜上皮细胞、成纤维细胞和真皮成纤维细胞中三种不同透明质酸合成酶表达的差异调节：使用实时 RT-PCR 进行定量分析。

• 发表时间: 2004
• 期刊: J Invest Dermatol 122
• 作者: Yoichi Yamada;Naoki Itano;Ken-Ichiro Hata;Minoru Ueda;Koji Kimata
• 通讯作者: Koji Kimata

生分解性樹脂複合材料により構成される組織再生用足場(スカフォールド)

可生物降解树脂复合材料组织再生支架

• 发表时间: 2004

Osteogenic potential of injectable tissue-engineered bone : a comparison among autogenous bone, bone substitute (Bio-Oss(^【○!R】)), platelet-rich plasma(PRP), and tissue-engineered bone with respect to their mechanical properties and histological findings

可注射组织工程骨的成骨潜力：自体骨、骨替代物（Bio-Oss（^【○！R】））、富血小板血浆（PRP）和组织工程骨的机械性能比较和组织学发现

• 发表时间: 2005
• 期刊: Journal of Biomedical Materials Research 73A
• 作者: Ito;K.;Yamada;Y.;Nagasaka;T.;Baba;S.;Ueda,M.
• 通讯作者: Ueda,M.