Development oh tissue-engineered bone with bio absovbency which holds occlusal support.

开发具有生物吸收性的组织工程骨，可提供咬合支撑。

• 批准号: 16591976
• 负责人: BABA Shunsuke
• 金额: $ 2.11万
• 依托单位: Foundation for Biomedical Research and Innovation
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591976/
• 关键词: Regenerative medicine; scafold

In this research, it is in the development of the scaffold to which it gives priority to a dynamic function of the bone by using the cultured cell and the biological absorption scaffold technology. The scaffold uses the growth of the osteobrast cell to treat to natural teeth and implant surroundings so that it may make amends for the cell when urge function is possessed and the reasonable amount is not obtained only by the cell. However, neither a hydroxyapatite nor a calcium phosphate system material that is the scaffold marketed now are appreciable in not the one in the point of regeneration of the bone that finishes being satisfactory but osteobrast cell formation. These problems are not to be able to promote the ability of the cell growth enough by the osteobrast cell of the self-origin and combining scaffolds. In addition, it is not a biological absorption scaffold to expect to replace native bone for a certain period in the function as the scaffold. Equip absorption, the inside p … More ore must be high, and the osteobrast cell and the blood vessel must go into it easily, and the structure with the function that the load of the occlusal force is possible in the mouth become conditions of functionally evaluating the reproductive bone. There is a feature in the point to reproduce surrounding bones of natural teeth an bones in the implant surroundings by MSCs of the self-origin that is the origin of the alveolar bone, enamel, the cement Quality, the periodontal membrane, and developing the culture bone of occlusal support that can be endured by using the scaffold with a biological absorption where the optimization design was done. In this research, by designing a biological absorption scaffold, it was assumed that the structure of the scaffold material corresponding to the revitalization degree of the culture osteobrast cell was able to be optimized, and the scaffold to be able to expect the healing of wound and the bone formation promotion action in addition to the more efficient bone reproduction was developed. The cell was settled on in the type scaffold and the plan sowing was settled on including knitting Cago referring to the result of a relation between PRP and the cell, and current clinical researches. This is the cloth structure finished knitting with one PLA fiber as a scaffold for the tissue regeneration. This scaffold has the cave internally in the Cago shape. It is because of raising the implantation rate by putting the cell in here. The one that has been put to practical use now forms the cell, and this scaffold can be cut into a necessary size by sowing internally in the reason to have the opening at the medical practice, and when operating, can be formed according to the coloboma part though is difficult sowing in the scaffold. Moreover, it is useful in the point that is the structure to which it can circulate around the body fluid by the interface in the scaffold and the coloboma part. Not only the bond efficiency of the cell to sow in the scaffold since the culture cell condition was examined to measure the optimization of the cell to sow here but also the mechanics characteristic of the scaffold having to take part became clear. In addition, there was a problem in bend strength was supplemented from the necessity for improving operativeness to surroundings of the PLA fiber with the binder of PCL. Moreover, the MSCs was bonded to squirrel-cage scaforld, and it cultured it. The biodegradation speed of the scaffold manufactured from PLA is slow as a result, externals remained being maintained for a long term of six months or more as for the scaffold though the cell reproduction and the differentiation induction to the osseous system were possible. The adjustment of the absorption degradation rate of the scaffold and the bone regeneration speed of the culture osteoblast became an important problem from these. When having experimented on the animal to confirm in Vivo of the PLA fiber, the reproduction of the bone would be confirmed in 12 weeks and the absorption of the scaffold was confirmed for that. An excellent result was obtained compared with the transplant only of the cell. Less

在这项研究中，正是在脚手架的发展中，通过使用培养的细胞和生物滥用脚手架技术，它优先于骨骼的动态功能。脚手架使用成骨细胞的生长来处理天然牙齿和植入物环境，以便在假定急切功能时可以对细胞进行修改，并且仅仅由细胞获得合理的量。但是，现在脚手架的氢氧化石或磷酸钙系统的材料都不是令人满意的，而在骨骼的再生点上都不令人满意，而是成骨细胞的形成。这些问题不能能够通过自根的成骨细胞和结合支架的成骨细胞来促进细胞生长的能力。另外，期望在该功能作为脚手架的一定时期内替换本地骨头并不是生物学惊喜。设备荒谬，内部p…更多的矿石必须高，成骨细胞和血管必须容易进入它，并且具有咬合力的载荷在口腔中的载荷成为可能在功能上评估复制骨骼的条件的功能的结构。在植入物周围的骨骼中，有一个自我原孔的骨头在植入周围的骨骼中的特征，这是牙槽骨，搪瓷，水泥质量，牙周膜的起源，并通过使用生物学意义上的生物学设计来实现咬合式支撑的培养骨，并在咬合骨骼中培养。在这项研究中，通过设计生物学惊喜脚手架，可以假定脚手架材料的结构与培养成骨细胞的振兴程度相对应的脚手架材料的结构能够优化，并且脚手架能够期望伤口的愈合和骨骼形成的愈合以及促进更有效的骨骼再生能力。该细胞在类型的支架中定位，计划播种的定位包括编织盒，指的是PRP与细胞之间关系的结果以及当前的临床研究。这是带有一种PLA纤维的布结构，作为组织修订的脚手架。这个脚手架在内部具有凯戈形状的山洞。这是因为通过将细胞放入此处提高植入率。现在已经进行了实际使用的一种细胞形成了细胞，并且可以通过内部播种，以便在医疗实践中开放，并且在运行时可以根据古罗巴马部分形成脚手架，但在脚手架中很难播种。此外，它在脚手架和古罗巴马部分的界面围绕体液循环的结构中很有用。由于检查了培养细胞条件以测量细胞以在此处播种的细胞的优化，而且还必须清楚脚手架的力学特征，因此不仅要检查细胞的培养细胞条件，因此细胞对脚手架的键效率不仅被检查。此外，从必要的特性中补充了弯曲强度的问题，这些特性将使用PCL的粘合剂改善PLA纤维周围的工作特性。此外，MSC被绑定到松鼠式的Scaforld，并将其培养。从PLA制造的脚手架的生物降解速度结果很慢，尽管细胞的繁殖和对骨系统的分化诱导是可能的，但对于脚手架而言，长期保持六个月或更长时间。脚手架的抽象降解率和培养成骨细胞的骨再生速度的调整成为这些重要问题。在对动物进行实验以确认PLA纤维的体内时，将在12周内确认骨骼的繁殖，并为此确认脚手架的抽象。与仅细胞的移植相比，获得了一个极好的结果。较少的

项目成果

Osteogenic potential of injectable tissue-engineered bone : a comparison among autogenous bone, bone substitute (Bio-Oss(^【○!R】)), platelet-rich plasma(PRP), and tissue-engineered bone with respect to their mechanical properties and histological findings

可注射组织工程骨的成骨潜力：自体骨、骨替代物（Bio-Oss（^【○！R】））、富血小板血浆（PRP）和组织工程骨的机械性能比较和组织学发现

• 发表时间: 2005
• 期刊: Journal of Biomedical Materials Research 73A
• 作者: Ito;K.;Yamada;Y.;Nagasaka;T.;Baba;S.;Ueda,M.
• 通讯作者: Ueda,M.

Differential Regulation by IL-10 and EGF of Expression Three Different Hyaluronan Synthases in Oral Mucosal of Epithelial Cells and Fibroblasts and Dermal Fibroblasts : Quantitative Analysis Using Real-Time RT-PCR.

IL-10 和 EGF 对上皮细胞、成纤维细胞和真皮成纤维细胞口腔粘膜中三种不同乙酰透明质酸合成酶表达的差异调节：使用实时 RT-PCR 进行定量分析。

• 发表时间: 2004
• 期刊: J Invest Dermatol 122
• 作者: Yoichi Yamada;Naoki Itano;Ken-Ichiro Hata;Minoru Ueda;Koji Kimata
• 通讯作者: Koji Kimata

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal Fibroblasts : Quantitative Analysis Using Real-Time RT-PCR.

IL-1β 和 EGF 对口腔粘膜上皮细胞、成纤维细胞和真皮成纤维细胞中三种不同透明质酸合成酶表达的差异调节：使用实时 RT-PCR 进行定量分析。

• 发表时间: 2004
• 期刊: J Invest Dermatol 122
• 作者: Yoichi Yamada;Naoki Itano;Ken-Ichiro Hata;Minoru Ueda;Koji Kimata
• 通讯作者: Koji Kimata

生分解性樹脂複合材料により構成される組織再生用足場(スカフォールド)

可生物降解树脂复合材料组织再生支架

• 发表时间: 2004

Osteogenic potential of injectable tissue-engineered bone : a comparison among autogenous bone, bone substitute (Bio-Oss(□)), platelet-rich plasma (PRP), and tissue-engineered bone with respect to their mechanical properties and histological findings

可注射组织工程骨的成骨潜力：自体骨、骨替代物（Bio-Oss（□））、富血小板血浆（PRP）和组织工程骨的机械性能和组织学结果的比较

• 发表时间: 2005
• 期刊: Journal of Biomedical Materials Research 73A
• 作者: I to;K.;Yamada;Y.;Nagasaka;T.;Baba;S.;Ueda;M.
• 通讯作者: M.