Development oh tissue-engineered bone with bio absovbency which holds occlusal support.

在本研究中，利用培养细胞和生物吸收支架技术开发了一种以骨的动态功能为主的支架材料。该支架利用成骨细胞的生长来治疗天然牙齿和种植环境，使其在具备催动功能时可以对细胞进行补偿，而不是仅由细胞获得合理的量。然而，无论是羟基磷灰石还是钙磷系统材料，无论是现在市场上销售的支架材料，都不是从骨再生的角度来看是令人满意的，而是在成骨细胞的形成方面。这些问题都是不能充分促进自体成骨细胞和复合支架的细胞生长能力的。此外，期望在一定时间内替代天然骨作为支架的生物吸收支架也不是生物吸收支架。装备吸收，内侧p…更多的矿石必须是高的，成骨细胞和血管必须容易进入其中，口腔内具有咬合力负荷功能的结构成为评价再生骨功能的条件。自体牙槽骨、牙釉质、牙骨质、牙周膜来源的MSCs在种植环境中复制天然牙周骨和种植环境中的骨有一个特点，即牙槽骨的来源、牙釉质的来源、牙周膜的来源、牙合支持的培养骨的形成以及利用具有生物吸收的支架进行优化设计。在本研究中，通过设计一种生物吸收支架，假设能够优化与培养的成骨细胞的活化程度相对应的支架材料的结构，在更高效的骨再生的同时，开发出能够预期伤口愈合和促进骨形成的支架。参考PRP与细胞关系的研究结果和目前的临床研究结果，将细胞安放在类型支架中，并确定计划播种，包括编织西红柿。这是用一根聚乳酸纤维作为组织再生支架编织完成的布料结构。这个脚手架内部有一个卡戈形状的洞穴。这是因为把细胞放在这里提高了植入率。已经投入实际使用的现在形成了细胞，该支架可以通过在内部播种来切割成必要的大小，以便在医疗实践中具有开口，并且在操作时，可以根据缺口部形成，尽管在支架中播种困难。此外，它的有用之处在于它可以通过支架和缺损部分中的界面在体液周围循环的结构。不仅考察了细胞培养条件后细胞在支架中的结合效率，衡量了细胞在支架中种植的优化程度，而且明确了支架必须参与的力学特性。此外，从提高聚乳酸纤维对周围环境的可操作性的必要性出发，补充了聚乳酸纤维在弯曲强度方面的问题。此外，将MSCs与松鼠笼支架结合，并进行培养。由聚乳酸制成的支架生物降解速度较慢，虽然可以进行细胞增殖和向骨系统诱导分化，但支架表面仍能保持长达6个月甚至更长时间。由此，支架的吸收降解率和培养成骨细胞的骨再生速度的调节成为一个重要的问题。当在动物身上进行实验以在体内确认聚乳酸纤维时，将在12周内确认骨骼的再生，并确认支架的吸收。与单纯细胞移植相比，获得了良好的效果。较少

In this research, it is in the development of the scaffold to which it gives priority to a dynamic function of the bone by using the cultured cell and the biological absorption scaffold technology. The scaffold uses the growth of the osteobrast cell to treat to natural teeth and implant surroundings so that it may make amends for the cell when urge function is possessed and the reasonable amount is not obtained only by the cell. However, neither a hydroxyapatite nor a calcium phosphate system material that is the scaffold marketed now are appreciable in not the one in the point of regeneration of the bone that finishes being satisfactory but osteobrast cell formation. These problems are not to be able to promote the ability of the cell growth enough by the osteobrast cell of the self-origin and combining scaffolds. In addition, it is not a biological absorption scaffold to expect to replace native bone for a certain period in the function as the scaffold. Equip absorption, the inside p … More ore must be high, and the osteobrast cell and the blood vessel must go into it easily, and the structure with the function that the load of the occlusal force is possible in the mouth become conditions of functionally evaluating the reproductive bone. There is a feature in the point to reproduce surrounding bones of natural teeth an bones in the implant surroundings by MSCs of the self-origin that is the origin of the alveolar bone, enamel, the cement Quality, the periodontal membrane, and developing the culture bone of occlusal support that can be endured by using the scaffold with a biological absorption where the optimization design was done. In this research, by designing a biological absorption scaffold, it was assumed that the structure of the scaffold material corresponding to the revitalization degree of the culture osteobrast cell was able to be optimized, and the scaffold to be able to expect the healing of wound and the bone formation promotion action in addition to the more efficient bone reproduction was developed. The cell was settled on in the type scaffold and the plan sowing was settled on including knitting Cago referring to the result of a relation between PRP and the cell, and current clinical researches. This is the cloth structure finished knitting with one PLA fiber as a scaffold for the tissue regeneration. This scaffold has the cave internally in the Cago shape. It is because of raising the implantation rate by putting the cell in here. The one that has been put to practical use now forms the cell, and this scaffold can be cut into a necessary size by sowing internally in the reason to have the opening at the medical practice, and when operating, can be formed according to the coloboma part though is difficult sowing in the scaffold. Moreover, it is useful in the point that is the structure to which it can circulate around the body fluid by the interface in the scaffold and the coloboma part. Not only the bond efficiency of the cell to sow in the scaffold since the culture cell condition was examined to measure the optimization of the cell to sow here but also the mechanics characteristic of the scaffold having to take part became clear. In addition, there was a problem in bend strength was supplemented from the necessity for improving operativeness to surroundings of the PLA fiber with the binder of PCL. Moreover, the MSCs was bonded to squirrel-cage scaforld, and it cultured it. The biodegradation speed of the scaffold manufactured from PLA is slow as a result, externals remained being maintained for a long term of six months or more as for the scaffold though the cell reproduction and the differentiation induction to the osseous system were possible. The adjustment of the absorption degradation rate of the scaffold and the bone regeneration speed of the culture osteoblast became an important problem from these. When having experimented on the animal to confirm in Vivo of the PLA fiber, the reproduction of the bone would be confirmed in 12 weeks and the absorption of the scaffold was confirmed for that. An excellent result was obtained compared with the transplant only of the cell. Less