Study of extremely low frequency magnetic fields-responding mechanism in neuron

神经元极低频磁场响应机制的研究

• 批准号: 17500250
• 负责人: HARADA Shinichi
• 金额: $ 2.24万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500250/
• 关键词: Calmodulin; microarray; RT-PCR; C. elegans; Calcium; gene expression; C.elegans; 低周波交流磁界; マイクロアレイ

The biological effect of extremely low frequency magnetic fields (ELFMFS) remains controversial. Some epidemiological studies have indicated positive correlations between magnetic fields and various types of cancer or other disease, including neurological disorders. To clarify the biological effects of ELFMFs, we performed the screening of ELFMFS-responding genes by using microarray technique in nematode C. elegans, as a model organism, and carried out the RT-PCR analysis not only the genes with significant difference identified by microarray, but also the associative and relative genes with possibility of ELFMFs-responding genes. Moreover, we investigated chemotactic response to either diacetyl or CuSO_4 after ELFMFs exposing.unc-43, one of the identified genes, is known to function as calmodulin-dependent protein kinase II (CaMKII), that mediates Ca^<2+> signal transmission in the nervous system of C. elegans. We found that unc-43 mRNA was down-regulated (2.4-fold) under ELFMFs exposure by RT-PCR. Another related genes, tax-6 (Calcineurin), itr-1 (IP3R) and cnb-1 (Calcineurin) in Ca^<2+>-Calmodulin (CaM) pathway were significantly down-regulated, respectively. ckk-1 (CaMKK), crh-1 (CREB) and cmk-1 (CaMKIV) showed no significant decrease (P<0.1), though these genes were reported to be functioning in the CaM pathway,.In behavioral analysis, chemotaxis index to diacetyl decreased, also chemotaxis index to CuSO_4 was shown weakly defective in avoidance when exposed with ELFMFs.These results suggest that the ELFMFs exposure caused the expression changes of neuronal specific genes which are associated with a part of calcium signal cascades, and has affected spontaneous locomotoion in C. elegans.

极低频磁场（ELFMFS）的生物效应仍然存在争议。一些流行病学研究表明磁场与各种类型的癌症或其他疾病（包括神经系统疾病）之间存在正相关性。为了阐明ELFMFs的生物学效应，我们利用微阵列技术以线虫C. elegans为模型生物进行ELFMFS反应基因的筛选，并对微阵列鉴定出的具有显着差异的基因以及可能是ELFMFs反应基因的关联和相关基因进行RT-PCR分析。此外，我们研究了 ELFMF 暴露后对二乙酰或 CuSO_4 的趋化反应。unc-43（已识别的基因之一）已知具有钙调蛋白依赖性蛋白激酶 II (CaMKII) 的功能，介导线虫神经系统中的 Ca^2+ 信号传递。我们通过 RT-PCR 发现，在 ELFMF 暴露下，unc-43 mRNA 下调（2.4 倍）。 Ca^2+-钙调蛋白(CaM)途径中的另一个相关基因tax-6(钙调神经磷酸酶)、itr-1(IP3R)和cnb-1(钙调神经磷酸酶)分别显着下调。 ckk-1 (CaMKK)、crh-1 (CREB) 和 cmk-1 (CaMKIV) 没有显着降低 (P<0.1)，尽管这些基因被报道在 CaM 通路中发挥作用。在行为分析中，当暴露于 ELFMF 时，对二乙酰的趋化指数降低，对 CuSO_4 的趋化指数在回避方面表现出弱缺陷。这些结果表明 ELFMF 暴露引起与部分钙信号级联相关的神经元特异性基因的表达变化，并影响线虫的自发运动。

项目成果

Neuronal organization of the rat inferior colliculus participating in four major auditory pathways

• DOI: 10.1016/j.heares.2006.04.004
• 发表时间: 2006-08-01
• 期刊: HEARING RESEARCH
• 影响因子: 2.8
• 作者: Okoyama, Shigeo;Ohbayashi, Masao;Harada, Shinichi
• 通讯作者: Harada, Shinichi

Neuronal organization of the rat inferio colliculus participating in four major auditor, pathways

大鼠下丘参与四大听觉通路的神经元组织

• 发表时间: 2006
• 期刊: Hearing Research 218
• 作者: Sakuta;H.;Takahashi;H.;Shintani;T.;Etani;K.;Aoshima;A.;Noda,M.;Shigeo Okoyama;Shigeo Okoyama
• 通讯作者: Shigeo Okoyama