Dynamic micro functional analysis of biological tissue underneath the skin surface by high-speed optical coherence tomography

通过高速光学相干断层扫描对皮肤表面下的生物组织进行动态微功能分析

• 批准号: 17500304
• 负责人: OHMI Masato
• 金额: $ 2.18万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500304/
• 关键词: Optical coherence tomography; High-resolution OCT; Femto-second laser; Optical constant of skin; Measurement of thickness of corneum

Our research target is demonstration of a novel high-resolution optical coherence tomography for measurement of optical constant of biological tissue as well as prediction of aging of human skin.(1)A mode-locked Ti : sapphire laser (femto-second laser) is used as the light source of the high-resolution OCT. The coherence length of femto-second laser of 3.1μm was obtained by compensation of unbalance of chromatic dispersion of the low-coherence interferometer. Furthermore, supercontinuum light source was obtained by use of a photonic crystal fiber.(2)In the longer wavelength region, a novel broadband light was used as the light source of ultra high-resolution OCT. The coherence length was 3.5μm at the center wavelength of 1.3μm. In vitro high-resolution OCT image of biological tissue was obtained.(3)In order to obtain in-focus imaging, we used the focusing effect of the focusing lens of the sample. This method is called in-focus OCT, in which confocal optics is combined with OCT imaging system. The imaging depth of more than 2.5mm was successfully obtained.(4)In the experiment, micro-skin structures including the epidermis, dermis and stratum basale are clearly observed using high-resolution OCT. In vivo high-resolution OCT of human skin was also obtained. The thickness of epidermis as well as stratum basale decreased with aging.(5)We developed the OCT probe for diagnosis of human skin. In the experiment, the OCT imaging with the OCT probe can provide clear imaging of the epidermis, dermis and stratum basale, showing that our probe is useful for diagnosis of aging of human skin.

我们的研究目标是展示一种新型的高分辨率光学相干层析技术，用于测量生物组织的光学常数以及预测人体皮肤的老化。(1)A锁模Ti：采用蓝宝石激光器（飞秒激光器）作为高分辨率OCT的光源，通过补偿低相干干涉仪的色散不平衡，获得了3.1μm的飞秒激光相干长度。此外，利用光子晶体光纤获得了超连续谱光源。(2)In在长波区，采用一种新型宽带光源作为超高分辨率OCT光源，在中心波长1.3μm处相干长度为3.5μm。获得了离体生物组织的高分辨率OCT图像。(3)In为了获得焦点对准成像，我们使用了样品的聚焦透镜的聚焦效果。将共焦光学系统与OCT成像系统相结合，实现了聚焦OCT成像。成功获得了大于2.5mm的成像深度。(4)In实验中，利用高分辨率OCT清晰地观察了皮肤的表皮、真皮和基底层等微观结构，并获得了人体皮肤的活体高分辨率OCT图像。表皮厚度和基底层厚度随年龄增长而减少。(5)We开发了用于人体皮肤诊断的OCT探头。在实验中，使用OCT探头的OCT成像可以提供表皮、真皮和基底层的清晰成像，表明我们的探头可以用于诊断人体皮肤的老化。

项目成果

光コヒーレンストモグラフィー

光学相干断层扫描

• 发表时间: 2007
• 期刊: 電子情報通信学会誌 Vol. 90, No. 3
• 作者: 春名正光

In-situ Observation of Tissue Laser Ablation Using Optical Coherence Tomography

• DOI: 10.1007/s11082-005-4189-2
• 发表时间: 2005-12
• 期刊: Optical and Quantum Electronics
• 影响因子: 3
• 作者: M. Ohmi;M. Tanizawa;A. Fukunaga;M. Haruna

低コヒーレンス光干渉を用いた生体機能検出

利用低相干光干涉进行生物功能检测

• 发表时间: 2006
• 期刊: 計測と制御 Vol. 45, No. 11
• 作者: 春名正光;Masato Ohmi;Masamitsu Haruna;M.Haruna;M.Haruna;藤 利栄;近江雅人;春名正光
• 通讯作者: 春名正光

In-situ Observation of biological Tissue in Laser Ablation Using Optical Coherence Tomography

使用光学相干断层扫描对激光烧蚀中的生物组织进行原位观察

• 发表时间: 2005
• 期刊: BME vol.43, no.4
• 作者: 春名正光;Masato Ohmi;Masamitsu Haruna;M.Haruna;M.Haruna;藤 利栄;近江雅人;春名正光;Masamitsu Haruna;Masamitsu Haruna;Toshie Fuji;Masato Ohmi;Masamitsu Haruna;Masamitsu Haruna;Masamitsu Haruna;Masato Ohmi;Masato Ohmi;Masamitsu Haruna;藤 利栄;春名正光;Masamitsu Haruna;Masamitsu Haruna;谷澤 学;Masato Ohmi;Masato Ohmi;Masato Ohmi;Kenji Nohara;Masato Ohmi;近江 雅人;Masato Ohmi;Masato Ohmi;Masato Ohmi;Kenji Nohara;Masato Ohmi;Masato Ohmi;Manabu Tanizawa
• 通讯作者: Manabu Tanizawa

OCTを用いた生体レーザアブレーションのin situ観察

使用 OCT 对生物激光烧蚀进行原位观察

• 发表时间: 2006
• 期刊: 生体医工学 Vol.43 No.4(掲載予定)
• 作者: 春名正光;Masato Ohmi;Masamitsu Haruna;M.Haruna;M.Haruna;藤 利栄;近江雅人;春名正光;Masamitsu Haruna;Masamitsu Haruna;Toshie Fuji;Masato Ohmi;Masamitsu Haruna;Masamitsu Haruna;Masamitsu Haruna;Masato Ohmi;Masato Ohmi;Masamitsu Haruna;藤 利栄;春名正光;Masamitsu Haruna;Masamitsu Haruna;谷澤 学
• 通讯作者: 谷澤 学