Bone Generation by using Three-Dimensional Geometrical Structural Controlled Cell Scaffold and Osteogenetic Growth Stimulated Factor Controlled Delivery System

采用机械化学方法将磷灰石水泥与胶原蛋白结合，制备新型自固化磷灰石/胶原复合水泥，并将美那曲酮（VK2）装入给药系统，在大鼠体内进行生物相容性测试。磷灰石/胶原复合水泥的体内水泥密度微射线图（CMM）结果表明，生物降解速率取决于水泥质量和纳米几何结构。负载VK2的磷灰石/胶原胶结物的CMM结果表明，胶结物的生物降解率显著依赖于它们的配方。基质磷灰石/胶原水泥的CMM增加至7 d后下降，骨样细胞在中心深度渗透。研究了一种新型磷酸钙（CaP）制剂改善去卵巢（OVX）大鼠骨矿物质缺乏的疗效。在pH为4.5、37℃的醋酸缓冲液中，对CaP制剂（G2:0.46%Mg、5.78%Zn、2.5%F; G3:3.1%Mg、0.03%Zn、3.01%F; G4:1.25%Mg、1.77%Zn、1.35%F）和Zn- tcp粉末（G1:6.17%Zn）的初始Mg、Zn离子释放率进行了实验。除GN和GC外，其余各组OVX大鼠右大腿注射CaP制剂（G2、G3、G4）和Zn-TCP （G1）粉组成的混悬液，每周1次，连续4周。GN大鼠和GC大鼠分别注射生理盐水。分析血浆锌和碱性磷酸盐水平。采用DEXA测量骨密度（BMD），采用三点弯曲分析测定骨（股骨）强度。G1、G2组血浆锌水平较高。G1、G2、GN组血浆锌曲线下面积显著大于G3、G4、GC组（p<0.05）。第28天，G1、G2、G3、G4组右股骨骨密度和骨机械强度均显著高于GC组。G1、G2、G3和G4组右股骨骨密度和骨机械强度均显著高于左股骨。结果表明，新型注射用CaP制剂能有效改善OVX大鼠的骨性能，可能用于骨质疏松症的治疗。少

Apatite cement and collagen were combined by a mechanochemical method to create a new self-setting apatite/collagen composite cement, and menatetrenone (VK2) was loaded into a drug delivery system to test biocompatibility in rats. The in-vivo cement density microradiograms (CMM) results of the apatite/collagen composite cements suggested that the biodegradation rate was dependent on the cement quality and nano-geometrical structure. The CMM result of VK2 loaded apatite/collagen cements suggested that the biodegradation rates of the cements were significantly dependent on their formulation. The CMM of ground apatite/collagen cement increased until 7 days and then decreased, and bone-like cells penetrated deeply in the center.The therapeutic efficacy of a new calcium phosphate (CaP)-based formulation in improving the bone mineral deficiency in ovariectomized (OVX) rats was investigated. The ions release experiments for CaP preparations (G2:0.46%Mg, 5.78%Zn, and 2.5%F; G3:3.1%Mg,0.03%Zn a … More nd 3.01%F ; G4:1.25%Mg,1.77%Zn,1.35%F) and of a Zn-TCP (G1:6.17%Zn) powders, the initial Mg and Zn ion release rates of MZF-CaPs were performed in acetate buffer at pH 4.5, 37℃. Suspensions consisting of CaP preparations (G2,G3,G4) and of a Zn-TCP (G1) powders were injected in the right thighs of OVX rats in all groups except for GN and GC, once a week for 4 weeks. GN and GC rats were injected with saline solutions. Plasma was analyzed for Zn land alkaline phosphates levels. The bone mineral density (BMD) was measured using DEXA and the bone (femur) strength determined using three-point-bending analysis. G1 and G2 groups showed high plasma Zn levels. The area under the curve of plasma Zn was significantly greater in the G1, G2, and GN groups than in the G3, G4, and GC groups (p<0.05). The BMD and bone mechanical strength of the right femur were significantly higher in the G1, G2, G3 and G4 groups than GC group on day 28. The right femur had significantly greater BMD and bone mechanical strength than the left femur in G1, G2, G3 and G4 groups. Results indicate that the new injectable CaP formulations are effective in improving bone properties of OVX rats and may be useful in osteoporosis therapy. Less