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Bone Generation by using Three-Dimensional Geometrical Structural Controlled Cell Scaffold and Osteogenetic Growth Stimulated Factor Controlled Delivery System

使用三维几何结构控制细胞支架和成骨生长刺激因子控制输送系统进行骨生成

基本信息

批准号：
17500322
负责人：
OTSUKA Makoto
金额：
$ 2.11万
依托单位：
Musashino University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Apatite cement and collagen were combined by a mechanochemical method to create a new self-setting apatite/collagen composite cement, and menatetrenone (VK2) was loaded into a drug delivery system to test biocompatibility in rats. The in-vivo cement density microradiograms (CMM) results of the apatite/collagen composite cements suggested that the biodegradation rate was dependent on the cement quality and nano-geometrical structure. The CMM result of VK2 loaded apatite/collagen cements suggested that the biodegradation rates of the cements were significantly dependent on their formulation. The CMM of ground apatite/collagen cement increased until 7 days and then decreased, and bone-like cells penetrated deeply in the center.The therapeutic efficacy of a new calcium phosphate (CaP)-based formulation in improving the bone mineral deficiency in ovariectomized (OVX) rats was investigated. The ions release experiments for CaP preparations (G2:0.46%Mg, 5.78%Zn, and 2.5%F; G3:3.1%Mg,0.03%Zn a … More nd 3.01%F ; G4:1.25%Mg,1.77%Zn,1.35%F) and of a Zn-TCP (G1:6.17%Zn) powders, the initial Mg and Zn ion release rates of MZF-CaPs were performed in acetate buffer at pH 4.5, 37℃. Suspensions consisting of CaP preparations (G2,G3,G4) and of a Zn-TCP (G1) powders were injected in the right thighs of OVX rats in all groups except for GN and GC, once a week for 4 weeks. GN and GC rats were injected with saline solutions. Plasma was analyzed for Zn land alkaline phosphates levels. The bone mineral density (BMD) was measured using DEXA and the bone (femur) strength determined using three-point-bending analysis. G1 and G2 groups showed high plasma Zn levels. The area under the curve of plasma Zn was significantly greater in the G1, G2, and GN groups than in the G3, G4, and GC groups (p<0.05). The BMD and bone mechanical strength of the right femur were significantly higher in the G1, G2, G3 and G4 groups than GC group on day 28. The right femur had significantly greater BMD and bone mechanical strength than the left femur in G1, G2, G3 and G4 groups. Results indicate that the new injectable CaP formulations are effective in improving bone properties of OVX rats and may be useful in osteoporosis therapy. Less

采用机械化学方法将磷灰石骨水泥与胶原蛋白结合，制备出新型自凝磷灰石/胶原复合骨水泥，并将四烯甲萘醌（VK2）负载到药物递送系统中，测试大鼠的生物相容性。磷灰石/胶原复合水泥的体内水泥密度显微放射图（CMM）结果表明，生物降解率取决于水泥质量和纳米几何结构。装载 VK2 的磷灰石/胶原骨水泥的 CMM 结果表明，水泥的生物降解率很大程度上取决于其配方。研磨磷灰石/胶原骨水泥的 CMM 一直增加到 7 天，然后下降，骨样细胞深入中心。研究了一种新的磷酸钙 (CaP) 配方在改善卵巢切除 (OVX) 大鼠骨矿物质缺乏方面的治疗效果。 CaP制剂（G2：0.46％Mg，5.78％Zn和2.5％F; G3：3.1％Mg，0.03％Zn a … More nd 3.01％F; G4：1.25％Mg，1.77％Zn，1.35％F）和Zn-TCP（G1：6.17％Zn）粉末的离子释放实验，初始Mg和锌离子释放率 MZF-CaPs 在 pH 4.5、37℃ 的醋酸盐缓冲液中进行。将由CaP制剂(G2、G3、G4)和Zn-TCP(G1)粉末组成的悬浮液注射到除GN和GC之外的所有组的OVX大鼠的右大腿中，每周一次，持续4周。 GN和GC大鼠注射盐水溶液。分析血浆中的锌和碱性磷酸盐水平。使用 DEXA 测量骨矿物质密度 (BMD)，并使用三点弯曲分析确定骨（股骨）强度。 G1 和 G2 组显示出高血浆锌水平。 G1、G2和GN组的血浆Zn曲线下面积显着大于G3、G4和GC组(p<0.05)。第28天，G1、G2、G3和G4组的右侧股骨BMD和骨机械强度显着高于GC组。G1、G2、G3和G4组右侧股骨的BMD和骨机械强度显着大于左侧股骨。结果表明，新的注射 CaP 制剂可有效改善 OVX 大鼠的骨特性，并可能用于骨质疏松症的治疗。较少的