DEVELOPMENT OF THE EARLY INDICATOR FOR LATE RADIATION DAMAGE OF KIDNEY

我们以前曾描述过放射后肾近端小管细胞增殖增加和异常大核的出现。提出了一个大核细胞可能正在死亡的模型，增殖增加可能导致这些细胞有丝分裂死亡并引起功能损伤。在9、12和15 Gy辐照后24小时，我们采用RT-PCR方法测量左肾与未辐照右肾的条带密度比，发现9 Gy照射后10个条带中有1个呈阳性，12 Gy照射后10个条带中有6个呈阳性，15 Gy照射后10个条带中有9个呈阳性。测量c-myc表达似乎是肾脏晚期放射损伤的潜在早期指标。

We have previously described the increased proliferation and appearance of abnormally large nuclei in renal proximal tubule cells after radiation. A model has been proposed that the large nuclear cells might be dying and the increased proliferation might lead to mitotic death of these cells and cause functional damage. We measured the amount of c-myc transcript taking the ratio of densities of bands for irradiated left kidney to unirradiated right kidney after RT-PCR procedure on 24 hours after 9,12 and 15 Gy irradiation, and found positive in 1 out of 10 after 9 Gy, 6 out of 10 after 12 Gy and 9 out of 10 after 15 Gy. Measuring c-myc expression seems to be a potential very early indicator of late radiation damage of kidney.