DEVELOPMENT OF THE EARLY INDICATOR FOR LATE RADIATION DAMAGE

晚期辐射损伤早期指标的开发

• 批准号: 10670858
• 负责人: OTSUKA Makoto
• 金额: $ 2.05万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670858/
• 关键词: radiation damage; late effect; mouse kidney; cell proliferation; c-myc; c-fos; BUN

We have previously described the increased proliferation and appearance of abnormally large nuclei in renal proximal tubule cells after radiation. A model has been proposed that the large nuclear cells might be dying and the increased proliferation might lead to mitotic death of these cells and cause functional damage. We measured the expression of c-fos and c-myc taking the ratio of densities of bands for irradiated portion to unirradiated portion of the kidney after RT-PCR procedure, and found the c-fos and c-myc expression increased very early (24 hours) after irradiation, probably representing increased DNA synthesis. The incidence of high c-myc expression increased according to radiation dose from 9 to 15 Gy. Then we measured the c-fos and c-myc expression 24 hours after 12 Gy irradiation, and followed BUN values representing the renal function of irradiated and right nephrectomized mice for 9 months after irradiation. Most of c-fos and c-myc positive mice showed increased BUN level, while all mice with negative c-fos and c-myc showed no BUN change. Measuring c-fos and c-myc expression seems to be a potential very early indicator of late radiation damage of kidney.

我们以前曾描述过放射后肾近曲小管细胞增殖增加和出现异常大的细胞核。已经提出了一种模型，即大的核细胞可能正在死亡，并且增加的增殖可能导致这些细胞的有丝分裂死亡并引起功能损伤。用RT-PCR方法检测照射后肾组织中c-fos和c-myc的表达，发现照射后早期（24小时）c-fos和c-myc的表达增加，可能与DNA合成增加有关。在9 ~ 15戈伊的照射剂量范围内，c-myc高表达的发生率随照射剂量的增加而增加。12戈伊照射后24 h检测c-fos和c-myc表达，并于照射后9个月随访BUN值。c-fos和c-myc阳性小鼠的BUN大多数升高，而c-fos和c-myc阴性小鼠的BUN均无变化。检测c-fos和c-myc的表达可能是肾放射损伤的早期指标。

项目成果

大塚誠: "腎晩発障害の早期指標としての増殖関連遺伝子"癌の臨床. 45. 267-268 (1999)

Makoto Otsuka：“增殖相关基因作为迟发性肾脏疾病的早期指标”，Cancer Clinic，45. 267-268 (1999)。

Makoto Otsuka: "Expression of K-ras, c-fos, and c-myc genes as a potential early indicator for late radiation damage of the kidney"Jpn J Cancer Clinics. 45-4. 267-268 (1999)

Makoto Otsuka：“K-ras、c-fos 和 c-myc 基因的表达作为肾脏晚期辐射损伤的潜在早期指标”Jpn J Cancer Clinics。

大塚 誠: "腎晩発障害の早期指標としての増殖関連遺伝子"癌の臨床. 45・4. 267-268 (1999)

Makoto Otsuka：“增殖相关基因作为迟发性肾脏疾病的早期指标”癌症诊所 45・4（1999）。

大塚 誠 他: "腎晩発障害の早期指標としての増殖関連遺伝子" 癌の臨床. 印刷中. (1999)

Makoto Otsuka 等人：“增殖相关基因作为晚期肾衰竭的早期指标”癌症临床杂志（1999 年）。