Research on the driving, lubrication and self assembled mechanism of bacterial flagellar motor

本研究旨在探讨细菌鞭毛马达的驱动、润滑和自组装机制，为纳米机器的构建提供新的理论依据。在纳米尺度的世界里，各种物理效应对机械的驱动、润滑和自组装机制起着至关重要的作用。我们在研究开始时并不知道构建非机器的指导原则。有鞭毛马达的细菌有时也被称为活纳米机器，因为它有马达、轴承万向节和螺钉。我们的主要目标是对细菌鞭毛马达的运动机理有一个准确的认识，并将其应用到纳米机器的驱动、润滑和自组装系统中。利用经典流体动力润滑理论，计算了负载电容。由此产生的负载电容为0.1pN（× 10 ^N）量级，不足以补偿驱动力（0.5pN），杆与轴颈之间的分子间力为10^2pN量级，远大于驱动力产生的负载，因此，必须引入新的润滑机制来解释PL环的行为。<-12>我们建议使用扩散双层作为润滑机制的可能候选者。我们估计的负载电容产生的这种效果，并表明足够的排斥力可以通过这种机制获得。我们制定了新的润滑机制，利用扩散的双电层，这是已知的起源的稳定性在疏水胶体溶液中的影响。结果表明，该机构不仅能提供足够的负载电容以补偿杆轴颈间的轴向力和径向力，而且能提供自组装系统的驱动力。

In this research, we investigated the driving, lubrication and self assembled mechanism of bacterial flagellar motor in order to develop new principle of nanomachine architecture. In nanoscale world, different kind of physical effects have essential roles on driving, lubrication and self assembled mechanism of machines. We did not know the guiding principle to construct nonomachine at the starting point of the study. Bacterial having flagellar motor sometimes called living nanomachine as it has motor, journal bearing universal joint and screw. Our main idea is to get the precise knowledge of the mechanism of bacterial flagellar motor and utilizing these mechanisms to the driving, lubrication and self assembled system of nanomachines.In a previous study, we estimated lubrication mechanism of bacterial flagellar. By using theory of the classical hydrodynamic lubrication, we calculated load capacitance. Resulting load capacitance is order of 0.1pN( x 10^<-12>N) and this values is not adequate to compensate driving force (0.5pN) Furthermore, intermolecular force between rod and journal becomes order of 10^2pN that is far bigger than load generated by driving force.Thus, we concluded that new lubrication mechanism must be introduced to explain the behavior of PL ring. We proposed use of diffusive double layer as possible candidate of lubrication mechanism. We estimated the load capacitance generated by this effect and showed the adequate amount of repulsive force can be obtained by this mechanism.We formulated new lubrication mechanism using the effects of diffusive double layer that is known as the origin of stability in hydrophobic colloidal solution. It is shown that this mechanism not only gives enough load capacitance to compensate the various forces between journal of rod in axial and radial direction but also gives driving force of self assembled system.