Research on the driving, lubrication and self assembled mechanism of bacterial flagellar motor

细菌鞭毛马达驱动、润滑及自组装机制研究

• 批准号: 17560115
• 负责人: NAKANO Takashi
• 金额: $ 2.3万
• 依托单位: Tokyo Institute of Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17560115/
• 关键词: Flagellar Motor; Diffusive Double Layer; Nano Machine; Non classical lubrication; Self Assembled System; PL ring; Universal Joing; Tribology; バイオナノマシン; トライボロジー

In this research, we investigated the driving, lubrication and self assembled mechanism of bacterial flagellar motor in order to develop new principle of nanomachine architecture. In nanoscale world, different kind of physical effects have essential roles on driving, lubrication and self assembled mechanism of machines. We did not know the guiding principle to construct nonomachine at the starting point of the study. Bacterial having flagellar motor sometimes called living nanomachine as it has motor, journal bearing universal joint and screw. Our main idea is to get the precise knowledge of the mechanism of bacterial flagellar motor and utilizing these mechanisms to the driving, lubrication and self assembled system of nanomachines.In a previous study, we estimated lubrication mechanism of bacterial flagellar. By using theory of the classical hydrodynamic lubrication, we calculated load capacitance. Resulting load capacitance is order of 0.1pN( x 10^<-12>N) and this values is not adequate to compensate driving force (0.5pN) Furthermore, intermolecular force between rod and journal becomes order of 10^2pN that is far bigger than load generated by driving force.Thus, we concluded that new lubrication mechanism must be introduced to explain the behavior of PL ring. We proposed use of diffusive double layer as possible candidate of lubrication mechanism. We estimated the load capacitance generated by this effect and showed the adequate amount of repulsive force can be obtained by this mechanism.We formulated new lubrication mechanism using the effects of diffusive double layer that is known as the origin of stability in hydrophobic colloidal solution. It is shown that this mechanism not only gives enough load capacitance to compensate the various forces between journal of rod in axial and radial direction but also gives driving force of self assembled system.

在本研究中，我们对细菌鞭毛马达的驱动、润滑和自组装机制进行了研究，以期开发出新的纳米机械结构原理。在纳米世界中，各种物理效应对机器的驱动、润滑和自组装机制起着至关重要的作用。在研究的起点上，我们并不知道构建非农机的指导原则。有鞭毛马达的细菌，有时称为活的纳米机器，因为它有马达、轴颈轴承万向节和螺丝。我们的主要思想是对细菌鞭毛马达的机制进行精确的了解，并利用这些机制来驱动、润滑和自组装纳米机械系统。在之前的研究中，我们对细菌鞭毛的润滑机制进行了估计。利用经典流体动力润滑理论，计算了负载电容。由此产生的载荷电容为0.1pN(x10^&lt；-12&gt；N)，不足以补偿驱动力(0.5pN)，此外，杆和轴颈之间的分子间力达到10^2pN量级，远远大于驱动力产生的载荷，因此，必须引入新的润滑机理来解释PL环的行为。提出了采用扩散双层作为可能的润滑机制。我们估计了这种效应产生的负载电容，并表明这种机制可以获得足够的排斥力。我们利用疏水胶体溶液中的扩散双层效应建立了新的润滑机理，这是稳定性的根源。结果表明，该机构不仅提供了足够的负载电容来补偿杆轴向轴向和径向的各种作用力，而且还给出了自组装系统的驱动力。

项目成果

Effects of Diffusive Double Layer on the Nano and Bio-nao Machines

扩散双层对纳米和生物纳米机器的影响

• 发表时间: 2005
• 期刊: International Tribology Conference KOBE 2005 Book of Synopses
• 作者: Takashi NAKANO;Satoshi MOMOZONO;Shin-ich AIZAWA;Hideyasu YOSHIMURA
• 通讯作者: Hideyasu YOSHIMURA

バクテリアべん毛モータの潤滑駆動機構を通して量子は見えるか

通过细菌鞭毛马达的润滑驱动机制可以看到量子吗？

• 发表时间: 2006
• 期刊: トライボロジー会議予稿集 東京 2006-5
• 作者: 中野隆;桃園聡;吉村英恭;相沢慎一
• 通讯作者: 相沢慎一

Can we observe the quantum mechanical effects through the and Hideyasu Yoshimura motion of bacterial flagellar motor

我们能否通过细菌鞭毛运动和吉村英康的运动来观察量子力学效应

• 期刊: Proceedings of JAST Tribology Conference Tokyo 2006-5
• 作者: Takashi NAKANO;Satoshi MOMOZON;Shin-ich Aizawa;Hideyasu Yoshimura
• 通讯作者: Hideyasu Yoshimura

バクテリアベん毛モータの運動を通して量子は見えるか

通过细菌鞭毛马达的运动可以看到量子吗？

• 发表时间: 2006
• 期刊: トライボロジー会議予稿集 2006-5
• 作者: 中野 隆;桃園 聡;吉村 英恭;相沢 慎一
• 通讯作者: 相沢 慎一

酸化チタン表面での水分子の分解機構の理論的研究II

氧化钛表面水分子分解机理的理论研究II

• 发表时间: 2005
• 期刊: 本コンピュータ化学会2005年秋季大会予稿集
• 作者: 中野 隆;森 和秀 他
• 通讯作者: 森 和秀 他